| 04 Apr 23 | r01     | Audax ItalianoAudax Italian | 0 - 1 | Newells Old BoysNewells Old B |
| 04 Apr 23 | r01 | GoiasGoias | 0 - 0 | Independiente Santa FeIndependiente |
| 05 Apr 23 | r01 | Estudiantes de Mérida F.CEstudiantes d | 0 - 1 | San LorenzoSan Lorenzo |
| 05 Apr 23 | r01 | Universidad César VallejoUniversidad C | 1 - 2 | LDU QuitoLDU Quito |
| 05 Apr 23 | r01 | BloomingBlooming | 0 - 1 | Santos FCSantos FC |
| 05 Apr 23 | r01 | Millonarios F.C.Millonarios F | 3 - 0 | Defensa y JusticiaDefensa y Jus |
| 05 Apr 23 | r01 | Oriente PetroleroOriente Petro | 0 - 1 | Estudiantes de la PlataEstudiantes d |
| 05 Apr 23 | r01 | FortalezaFortaleza | 4 - 0 | PalestinoPalestino |
| 05 Apr 23 | r01 | DanubioDanubio | 2 - 0 | EmelecEmelec |
| 06 Apr 23 | r01 | America MGAmerica MG | 4 - 1 | PeñarolPeñarol |
| 06 Apr 23 | r01 | Gimnasia LPGimnasia LP | 0 - 1 | UniversitarioUniversitario |
| 06 Apr 23 | r01 | HuracanHuracan | 4 - 1 | Club GuaraníClub Guaraní |
| 07 Apr 23 | r01 | TacuaryTacuary | 1 - 4 | BragantinoBragantino |
| 07 Apr 23 | r01 | TigreTigre | 0 - 2 | Sao PauloSao Paulo |
| 07 Apr 23 | r01 | MagallanesMagallanes | 2 - 2 | Botafogo RJBotafogo RJ |
| 07 Apr 23 | r01 | Puerto Cabello F.C.Puerto Cabell | 0 - 2 | Deportes TolimaDeportes Toli |
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