| 09 May 26 |
r00        | Philadelphia Lone Star FCPhiladelphia | 3 - 1 | West Chester UnitedWest Chester |
| 09 May 26 |
r00        | Boston CityBoston City | 0 - 2 | New England FCNew England F |
| 09 May 26 |
r00        | Apotheos FCApotheos FC | 1 - 2 | Columbus UnitedColumbus Unit |
| 10 May 26 |
r00        | Almaden FCAlmaden FC | 2 - 3 | San Francisco City FCSan Francisco |
| 10 May 26 |
r00        | St. Louis AmbushSt. Louis Amb | 1 - 3 | Peoria CityPeoria City |
| 10 May 26 |
r00        | Fort Worth VaquerosFort Worth Va | 0 - 4 | Denton DiablosDenton Diablo |
| 10 May 26 |
r00        | FC OlympiaFC Olympia | 0 - 3 | Portland BangersPortland Bang |
| 10 May 26 |
r00        | Loudoun United 2Loudoun Unite | 4 - 5 | Christos FCChristos FC |
| 10 May 26 |
r00        | Northern Virginia FCNorthern Virg | 3 - 0 | Virginia MaraudersVirginia Mara |
| 11 May 26 |
r00        | West Seattle JunctionWest Seattle | 0 - 0 | Tacoma StarsTacoma Stars |
| 12 May 26 |
r00        | Lehigh Valley UnitedLehigh Valley | 3 - 1 | West Chester UnitedWest Chester |
| 13 May 26 |
r00        | Dayton Dutch LionsDayton Dutch | 1 - 5 | Louisville City IILouisville Ci |
| 13 May 26 |
r00        | AppalachianAppalachian | 0 - 0 | Asheville City SCAsheville Cit |
| 13 May 26 |
r00        | Brevard SCBrevard SC | 2 - 3 | Lakeland UnitedLakeland Unit |
| 13 May 26 |
r00        | Kalamazoo FCKalamazoo FC | 1 - 2 | Union FC MacombUnion FC Maco |
| 13 May 26 |
r00        | Seacoast United PhantomsSeacoast Unit | 2 - 3 | New England FCNew England F |
| 14 May 26 |
r00        | Wake FCWake FC | 1 - 5 | Charlotte EaglesCharlotte Eag |
| 15 May 26 |
r00        | West Virginia UnitedWest Virginia | 2 - 0 | Kings Hammer ColumbusKings Hammer |
| 15 May 26 |
r00        | Western Mass PioneersWestern Mass | 4 - 2 | Albany RushAlbany Rush |
| 16 May 26 |
r00        | Des Moines MenaceDes Moines Me | 2 - 3 | Santafé WanderersSantafé Wande |
| 16 May 26 |
r00        | San Antonio FC 2San Antonio F | 1 - 3 | Laredo HeatLaredo Heat |
| 16 May 26 |
r00        | Edgewater CastleEdgewater Cas | 4 - 2 | Rockford RaptorsRockford Rapt |
| 16 May 26 |
r00        | Asheville City SCAsheville Cit | 0 - 1 | Hickory FCHickory FC |
| 16 May 26 |
r00        | Seacoast United PhantomsSeacoast Unit | 0 - 2 | Vermont GreenVermont Green |
| 16 May 26 |
r00        | Philadelphia Lone Star FCPhiladelphia | - | Eagle FCEagle FC |
| 16 May 26 |
r00        | Midwest UnitedMidwest Unite | 0 - 1 | Lansing City FootballLansing City |
| 16 May 26 |
r00        | Minneapolis CityMinneapolis C | 6 - 0 | Chicago City Dutch LionsChicago City |
| 16 May 26 |
r00        | Staten Island AthleticStaten Island | 2 - 3 | Ironbound SCIronbound SC |
| 16 May 26 |
r00        | Birmingham Legion 2Birmingham Le | 1 - 1 | East AtlantaEast Atlanta |
| 16 May 26 |
r00        | Christos FCChristos FC | 2 - 1 | Lionsbridge FCLionsbridge F |
| 16 May 26 |
r00        | Charlotte Independence IICharlotte Ind | 3 - 1 | South Carolina UnitedSouth Carolin |
| 16 May 26 |
r00        | Connecticut RushConnecticut R | 1 - 1 | Boston CityBoston City |
| 16 May 26 |
r00        | Hill CityHill City | 4 - 1 | Loudoun United 2Loudoun Unite |
| 16 May 26 |
r00        | Oakland CountyOakland Count | 4 - 0 | Union FC MacombUnion FC Maco |
| 16 May 26 |
r00        | Shark CoastShark Coast | 1 - 1 | Sporting JAX IISporting JAX |
| 16 May 26 |
r00        | Weston FCWeston FC | 0 - 2 | Inter Miami IIInter Miami I |
| 16 May 26 |
r00        | New England FCNew England F | 4 - 1 | AC ConnecticutAC Connecticu |
| 16 May 26 |
r00        | Columbus UnitedColumbus Unit | 1 - 1 | Apotheos FCApotheos FC |
| 17 May 26 |
r00        | Dothan UnitedDothan United | 2 - 1 | Montgomery UnitedMontgomery Un |
| 17 May 26 |
r00        | Red RiverRed River | 2 - 2 | Hattiesburg FCHattiesburg F |
| 17 May 26 |
r00        | Sunflower StateSunflower Sta | 1 - 4 | St. Louis AmbushSt. Louis Amb |
| 17 May 26 |
r00        | Louisiana KreweLouisiana Kre | 1 - 0 | Memphis FCMemphis FC |
| 17 May 26 |
r00        | Fort Worth VaquerosFort Worth Va | 0 - 1 | Lubbock MatadorsLubbock Matad |
| 17 May 26 |
r00        | McKinney ChupacabrasMcKinney Chup | 2 - 1 | Denton DiablosDenton Diablo |
| 17 May 26 |
r00        | Portland BangersPortland Bang | 1 - 0 | Snohomish UnitedSnohomish Uni |
| 17 May 26 |
r00        | Southern California EagleSouthern Cali | 3 - 1 | AMSG FCAMSG FC |
| 17 May 26 |
r00        | Pittsburgh Riverhounds 2Pittsburgh Ri | 5 - 0 | Cleveland ForceCleveland For |
| 17 May 26 |
r00        | San Francisco City FCSan Francisco | 3 - 0 | Davis LegacyDavis Legacy |
| 18 May 26 |
r00        | AHFC RoyalsAHFC Royals | 3 - 0 | Hill Country LobosHill Country |
| 18 May 26 |
r00        | Charlottesville BluesCharlottesvil | 0 - 1 | Annapolis BluesAnnapolis Blu |
| 19 May 26 |
r00        | Boston BoltsBoston Bolts | 1 - 4 | Vermont GreenVermont Green |
| 19 May 26 |
r00        | Lehigh Valley UnitedLehigh Valley | 2 - 1 | Delaware FCDelaware FC |
| 19 May 26 |
r00        | Charlotte EaglesCharlotte Eag | 0 - 4 | AppalachianAppalachian |
| 20 May 26 |
r00        | Fort Worth VaquerosFort Worth Va | 0 - 7 | TexomaTexoma |
| 20 May 26 |
r00        | Midlakes UnitedMidlakes Unit | 3 - 0 | FC OlympiaFC Olympia |
| 20 May 26 |
r00        | Southern California EagleSouthern Cali | 2 - 1 | City SCCity SC |
| 20 May 26 |
r00        | Flint City BucksFlint City Bu | 2 - 1 | Lansing City FootballLansing City |
| 20 May 26 |
r00        | New Jersey CopaNew Jersey Co | 0 - 2 | Long Island Rough RidersLong Island R |
| 20 May 26 |
r00        | Louisville City IILouisville Ci | 0 - 2 | West Virginia UnitedWest Virginia |
| 20 May 26 |
r00        | Steel CitySteel City | 0 - 0 | Lorain County LeviathanLorain County |
| 20 May 26 |
r00        | Albany RushAlbany Rush | 0 - 4 | New England FCNew England F |
| 20 May 26 |
r00        | Inter Miami IIInter Miami I | 2 - 0 | Brevard SCBrevard SC |
| 20 May 26 |
r00        | North Carolina FC U23North Carolin | 1 - 3 | Hickory FCHickory FC |
| 20 May 26 |
r00        | AC ConnecticutAC Connecticu | 1 - 3 | Connecticut RushConnecticut R |
| 20 May 26 |
r00        | Akron CityAkron City | 1 - 4 | Cleveland ForceCleveland For |
| 20 May 26 |
r00        | Lakeland UnitedLakeland Unit | 2 - 3 | FC Miami CityFC Miami City |
| 20 May 26 |
r00        | Lionsbridge FCLionsbridge F | 3 - 0 | Virginia MaraudersVirginia Mara |
| 20 May 26 |
r00        | Patuxent Football AthletiPatuxent Foot | 1 - 3 | Charlottesville BluesCharlottesvil |
| 20 May 26 |
r00        | Seacoast United PhantomsSeacoast Unit | 0 - 1 | Western Mass PioneersWestern Mass |
| 20 May 26 |
r00        | Tobacco RoadTobacco Road | 0 - 1 | Charlotte Independence IICharlotte Ind |
| 20 May 26 |
r00        | Cedar Stars RushCedar Stars R | 2 - 1 | Manhattan SCManhattan SC |
| 20 May 26 |
r00        | East AtlantaEast Atlanta | 0 - 0 | Columbus UnitedColumbus Unit |
| 20 May 26 |
r00        | Louisiana KreweLouisiana Kre | 2 - 3 | Mississippi BrillaMississippi B |
| 21 May 26 |
r00        | St. Croix LegendsSt. Croix Leg | 0 - 6 | Sueño FCSueño FC |
| 21 May 26 |
r00        | AHFC RoyalsAHFC Royals | 6 - 1 | Twin City ToucansTwin City Tou |
| 21 May 26 |
r00        | Springfield FCSpringfield F | 0 - 2 | St. Louis AmbushSt. Louis Amb |
| 21 May 26 |
r00        | San Francisco GlensSan Francisco | 1 - 2 | Marin FC LegendsMarin FC Lege |
| 21 May 26 |
r00        | Portland BangersPortland Bang | 3 - 1 | West Seattle JunctionWest Seattle |
| 21 May 26 |
r00        | Brooke HouseBrooke House | 0 - 1 | Sporting JAX IISporting JAX |
| 21 May 26 |
r00        | Oakland CountyOakland Count | 2 - 1 | Kalamazoo FCKalamazoo FC |
| 21 May 26 |
r00        | Pittsburgh Riverhounds 2Pittsburgh Ri | 2 - 0 | Erie Sports CenterErie Sports C |
| 22 May 26 |
r00        | Motown STAMotown STA | 6 - 0 | Westchester FlamesWestchester F |
| 22 May 26 |
r00        | Laredo HeatLaredo Heat | 4 - 0 | San Antonio FC 2San Antonio F |
| 22 May 26 |
r00        | Ocean City Nor'eastersOcean City No | 3 - 0 | Philadelphia Lone Star FCPhiladelphia |