| 10 May 24 |
r01        | Louisiana Krewe FCLouisiana Kre | 3 - 1 | Blue Goose SCBlue Goose SC |
| 11 May 24 |
r01        | Philadelphia Lone Star FCPhiladelphia | 0 - 5 | West Chester United IIWest Chester |
| 11 May 24 |
r01        | San Francisco City FCSan Francisco | 2 - 0 | Project 51OProject 51O |
| 11 May 24 |
r01        | North Carolina Fusion U23North Carolin | 3 - 0 | Charlotte Independence IICharlotte Ind |
| 11 May 24 |
r01        | Virginia Beach United FCVirginia Beac | 0 - 1 | Lionsbridge FCLionsbridge F |
| 11 May 24 |
r01        | Lansing City FootballLansing City | 0 - 0 | Midwest United FCMidwest Unite |
| 12 May 24 |
r01        | Corpus Christi FCCorpus Christ | 3 - 0 | Houston FCHouston FC |
|
| |
| 12 May 24 |
r02        | Des Moines MenaceDes Moines Me | 1 - 1 | Peoria CityPeoria City |
|
| |
| 12 May 24 |
r01        | Almaden FCAlmaden FC | 2 - 1 | San Francisco GlensSan Francisco |
| 14 May 24 |
r01        | Charlotte Independence IICharlotte Ind | 3 - 4 | Charlotte EaglesCharlotte Eag |
| 14 May 24 |
r01        | Boston BoltsBoston Bolts | 0 - 1 | Western Mass PioneersWestern Mass |
| 15 May 24 |
r01        | North Carolina FC U23North Carolin | 2 - 0 | West Virginia UnitedWest Virginia |
| 15 May 24 |
r01        | North Carolina Fusion U23North Carolin | 1 - 0 | Tobacco Road FCTobacco Road |
| 15 May 24 |
r01        | Virginia Beach United FCVirginia Beac | 1 - 0 | Northern Virginia FCNorthern Virg |
|
| |
| 15 May 24 |
r02        | Midwest United FCMidwest Unite | 1 - 0 | South Bend Lions FCSouth Bend Li |
| 16 May 24 |
r02        | Chicago Dutch Lions FCChicago Dutch | 0 - 5 | Des Moines MenaceDes Moines Me |
|
| |
| 16 May 24 |
r01        | Davis Legacy SCDavis Legacy | 2 - 2 | San Francisco GlensSan Francisco |
| 17 May 24 |
r01        | Southern California SeahoSouthern Cali | 0 - 1 | Ventura County FusionVentura Count |
|
| |
| 17 May 24 |
r02        | Southern Soccer AcademySouthern Socc | 0 - 5 | Asheville City SCAsheville Cit |
| 17 May 24 |
r02        | Charlotte EaglesCharlotte Eag | 4 - 0 | Tobacco Road FCTobacco Road |
| 17 May 24 |
r02        | Kalamazoo FCKalamazoo FC | 1 - 0 | Lansing City FootballLansing City |
| 18 May 24 |
r02        | Philadelphia Lone Star FCPhiladelphia | 4 - 0 | Real Central New JerseyReal Central |
| 18 May 24 |
r02        | Kings Hammer FCKings Hammer | 1 - 1 | Cleveland Force SCCleveland For |
| 18 May 24 |
r02        | Northern Virginia FCNorthern Virg | 2 - 1 | Patuxent Football AthletiPatuxent Foot |
| 18 May 24 |
r02        | Midwest United FCMidwest Unite | 3 - 0 | AFC Ann ArborAFC Ann Arbor |
| 18 May 24 |
r02        | Seacoast United PhantomsSeacoast Unit | 6 - 1 | AC ConnecticutAC Connecticu |
| 18 May 24 |
r02        | North Carolina FC U23North Carolin | 4 - 0 | Charlotte Independence IICharlotte Ind |
| 18 May 24 |
r02        | West Virginia UnitedWest Virginia | 1 - 1 | North Carolina Fusion U23North Carolin |
| 18 May 24 |
r02        | Morris Elite SCMorris Elite | 0 - 1 | Staten Island AthleticStaten Island |
|
| |
| 18 May 24 |
r03        | Lehigh Valley UnitedLehigh Valley | 0 - 2 | Reading United ACReading Unite |
| 18 May 24 |
r03        | Western Mass PioneersWestern Mass | 4 - 0 | FC Málaga City New YorkFC Málaga Cit |
|
| |
| 18 May 24 |
r02        | Wake FCWake FC | 2 - 6 | SC United BantamsSC United Ban |
| 18 May 24 |
r02        | South Bend Lions FCSouth Bend Li | 1 - 2 | Flint City BucksFlint City Bu |
| 18 May 24 |
r02        | Fort Wayne FCFort Wayne FC | 3 - 1 | Dayton Dutch LionsDayton Dutch |
| 18 May 24 |
r02        | New Mexico United U-23New Mexico Un | 2 - 1 | Colorado Rush SCColorado Rush |
| 18 May 24 |
r02        | Virginia Beach UnitedVirginia Beac | 3 - 0 | Charlottesville BluesCharlottesvil |
|
| |
| 18 May 24 |
r03        | Ironbound SCIronbound SC | 0 - 4 | Hudson Valley HammersHudson Valley |
|
| |
| 19 May 24 |
r02        | Blue Goose SCBlue Goose SC | 0 - 0 | Mississippi Brilla FCMississippi B |
| 19 May 24 |
r02        | Louisiana Krewe FCLouisiana Kre | 0 - 0 | Little Rock RangersLittle Rock R |
| 19 May 24 |
r02        | Corpus Christi FCCorpus Christ | 1 - 0 | AC Houston SurAC Houston Su |
|
| |
| 19 May 24 |
r03        | Bavarian UnitedBavarian Unit | 1 - 0 | Minneapolis CityMinneapolis C |
| 19 May 24 |
r03        | Peoria CityPeoria City | 4 - 0 | Chicago Dutch Lions FCChicago Dutch |
| 19 May 24 |
r03        | Long Island Rough RidersLong Island R | 2 - 1 | New Jersey Copa FCNew Jersey Co |
| 19 May 24 |
r03        | Boston City FCBoston City F | 0 - 3 | Vermont Green FCVermont Green |
| 19 May 24 |
r03        | Cedar Stars RushCedar Stars R | 3 - 0 | Manhattan SCManhattan SC |
|
| |
| 20 May 24 |
r02        | Davis Legacy SCDavis Legacy | 3 - 1 | Marin FC LegendsMarin FC Lege |
| 20 May 24 |
r02        | Florida Elite Soccer AcadFlorida Elite | 1 - 1 | The Villages SCThe Villages |
|
| |
| 21 May 24 |
r03        | Charlotte EaglesCharlotte Eag | 0 - 3 | North Carolina FC U23North Carolin |
| 21 May 24 |
r03        | West Chester United IIWest Chester | 2 - 2 | Philadelphia Lone Star FCPhiladelphia |
| 22 May 24 |
r03        | Black Rock FCBlack Rock FC | 0 - 2 | Vermont Green FCVermont Green |
|
| |
| 22 May 24 |
r04        | Charlotte Independence IICharlotte Ind | 3 - 1 | Wake FCWake FC |
|
| |
| 22 May 24 |
r03        | Nona FCNona FC | 2 - 2 | Florida Elite Soccer AcadFlorida Elite |
|
| |
| 22 May 24 |
r04        | Real Central New JerseyReal Central | 1 - 0 | Lehigh Valley UnitedLehigh Valley |
| 22 May 24 |
r04        | Albany RushAlbany Rush | 0 - 4 | Western Mass PioneersWestern Mass |
| 22 May 24 |
r04        | Boston BoltsBoston Bolts | 1 - 1 | AC ConnecticutAC Connecticu |
| 22 May 24 |
r04        | Seacoast United PhantomsSeacoast Unit | 4 - 0 | Boston City FCBoston City F |
| 22 May 24 |
r04        | Tobacco Road FCTobacco Road | 1 - 1 | SC United BantamsSC United Ban |
| 22 May 24 |
r04        | Virginia Beach United FCVirginia Beac | 4 - 1 | Patuxent Football AthletiPatuxent Foot |
|
| |
| 22 May 24 |
r03        | AFC Ann ArborAFC Ann Arbor | 2 - 2 | Kalamazoo FCKalamazoo FC |
| 22 May 24 |
r03        | Kings Hammer FCKings Hammer | 0 - 1 | Fort Wayne FCFort Wayne FC |
|
| |
| 22 May 24 |
r04        | Hudson Valley HammersHudson Valley | 3 - 0 | Staten Island AthleticStaten Island |
|
| |
| 22 May 24 |
r03        | Midwest United FCMidwest Unite | 2 - 1 | Oakland County FCOakland Count |
| 23 May 24 |
r04        | Westchester FlamesWestchester F | 2 - 0 | New Jersey Copa FCNew Jersey Co |
| 23 May 24 |
r04        | Minneapolis CityMinneapolis C | 0 - 3 | St. Croix SCSt. Croix SC |
| 23 May 24 |
r04        | Chicago Dutch Lions FCChicago Dutch | 0 - 6 | Peoria CityPeoria City |
|
| |
| 24 May 24 |
r03        | Colorado Rush SCColorado Rush | 2 - 2 | Ogden City SCOgden City SC |
|
| |
| 24 May 24 |
r05        | Tobacco Road FCTobacco Road | 0 - 4 | North Carolina FC U23North Carolin |
|
| |
| 24 May 24 |
r03        | Asheville City SCAsheville Cit | 4 - 1 | Southern Soccer AcademySouthern Socc |
| 25 May 24 |
r03        | Ventura County FusionVentura Count | 0 - 0 | FC TucsonFC Tucson |
| 25 May 24 |
r03        | Almaden FCAlmaden FC | 0 - 0 | San Francisco City FCSan Francisco |
|
| |
| 25 May 24 |
r05        | Vermont Green FCVermont Green | 1 - 1 | FC Málaga City New YorkFC Málaga Cit |
| 25 May 24 |
r05        | Morris Elite SCMorris Elite | 0 - 2 | Cedar Stars RushCedar Stars R |
| 25 May 24 |
r05        | Albany RushAlbany Rush | 2 - 2 | Black Rock FCBlack Rock FC |
|
| |
| 25 May 24 |
r04        | AFC Ann ArborAFC Ann Arbor | 1 - 0 | Oakland County FCOakland Count |
|
| |
| 25 May 24 |
r05        | New Jersey Copa FCNew Jersey Co | 1 - 2 | Staten Island AthleticStaten Island |
| 25 May 24 |
r05        | West Virginia UnitedWest Virginia | 3 - 1 | Wake FCWake FC |
|
| |
| 25 May 24 |
r04        | Miami ACMiami AC | 1 - 1 | Tampa Bay United SCTampa Bay Uni |
|
| |
| 25 May 24 |
r05        | Ocean City NoreastersOcean City No | 4 - 1 | Philadelphia Lone Star FCPhiladelphia |
| 25 May 24 |
r05        | Charlotte EaglesCharlotte Eag | 2 - 2 | North Carolina Fusion U23North Carolin |
| 25 May 24 |
r05        | SC United BantamsSC United Ban | 1 - 0 | Charlotte Independence IICharlotte Ind |
|
| |
| 25 May 24 |
r04        | South Bend Lions FCSouth Bend Li | 0 - 4 | Kalamazoo FCKalamazoo FC |
| 25 May 24 |
r04        | Flint City BucksFlint City Bu | 0 - 0 | Lansing City FootballLansing City |
| 25 May 24 |
r04        | Dayton Dutch LionsDayton Dutch | 0 - 1 | Cleveland Force SCCleveland For |
| 25 May 24 |
r04        | Fort Wayne FCFort Wayne FC | 1 - 0 | Toledo Villa FCToledo Villa |
|
| |
| 25 May 24 |
r06        | West Chester United IIWest Chester | 2 - 1 | Reading United ACReading Unite |
| 25 May 24 |
r06        | FC Motown IIFC Motown II | 1 - 3 | Long Island Rough RidersLong Island R |
|
| |
| 26 May 24 |
r04        | Mississippi Brilla FCMississippi B | 0 - 2 | Little Rock RangersLittle Rock R |
|
| |
| 26 May 24 |
r05        | Des Moines MenaceDes Moines Me | 4 - 1 | Chicago City SCChicago City |
|
| |
| 26 May 24 |
r04        | Davis Legacy SCDavis Legacy | 2 - 5 | Project 51OProject 51O |
| 26 May 24 |
r04        | Oly Town FCOly Town FC | 1 - 1 | Lane United FCLane United F |
|
| |
| 26 May 24 |
r06        | Patuxent Football AthletiPatuxent Foot | 2 - 2 | Christos FCChristos FC |
| 26 May 24 |
r06        | Lionsbridge FCLionsbridge F | 0 - 2 | Charlottesville BluesCharlottesvil |
|
| |
| 27 May 24 |
r04        | AC Houston SurAC Houston Su | 0 - 2 | Twin City ToucansTwin City Tou |
|
| |
| 28 May 24 |
r06        | Vermont Green FCVermont Green | 1 - 0 | Black Rock FCBlack Rock FC |
|
| |
| 29 May 24 |
r05        | Tennessee SCTennessee SC | 2 - 4 | East Atlanta FCEast Atlanta |
|
| |
| 29 May 24 |
r06        | Christos FCChristos FC | 1 - 1 | Virginia Beach United FCVirginia Beac |