| 07 May 23 |
r01        | Louisiana Krewe FCLouisiana Kre | 11 - 0 | Blue Goose SCBlue Goose SC |
| 08 May 23 |
r01        | Project 51OProject 51O | 6 - 2 | Davis Legacy SCDavis Legacy |
| 10 May 23 |
r01        | North Carolina Fusion U23North Carolin | 1 - 0 | Charlotte Independence IICharlotte Ind |
| 13 May 23 |
r01        | Midwest United FCMidwest Unite | 0 - 0 | Oakland County FCOakland Count |
| 13 May 23 |
r01        | Philadelphia Lone Star FCPhiladelphia | 3 - 0 | Lehigh Valley UnitedLehigh Valley |
| 13 May 23 |
r01        | Louisiana Krewe FCLouisiana Kre | 4 - 1 | LA Parish ACLA Parish AC |
|
| |
| 14 May 23 |
r02        | Minneapolis CityMinneapolis C | 1 - 2 | St. Croix SCSt. Croix SC |
| 14 May 23 |
r02        | AFC Ann ArborAFC Ann Arbor | 5 - 0 | Kalamazoo FCKalamazoo FC |
| 14 May 23 |
r02        | Fort Wayne FCFort Wayne FC | 1 - 0 | Kings Hammer FCKings Hammer |
|
| |
| 14 May 23 |
r01        | Northern Virginia FCNorthern Virg | 1 - 0 | Patuxent Football AthletiPatuxent Foot |
|
| |
| 14 May 23 |
r02        | Des Moines MenaceDes Moines Me | 3 - 0 | FC WichitaFC Wichita |
|
| |
| 14 May 23 |
r01        | Little Rock RangersLittle Rock R | 7 - 0 | Blue Goose SCBlue Goose SC |
| 14 May 23 |
r01        | Virginia Beach United FCVirginia Beac | 0 - 2 | Lionsbridge FCLionsbridge F |
| 14 May 23 |
r02        | San Francisco City FCSan Francisco | 2 - 1 | Project 51OProject 51O |
|
| |
| 16 May 23 |
r01        | Charlotte Independence IICharlotte Ind | 1 - 2 | West Virginia UnitedWest Virginia |
|
| |
| 17 May 23 |
r02        | Boston BoltsBoston Bolts | 0 - 3 | Western Mass PioneersWestern Mass |
| 17 May 23 |
r02        | Charlotte EaglesCharlotte Eag | 1 - 1 | North Carolina FC U23North Carolin |
| 17 May 23 |
r02        | Albany RushAlbany Rush | 2 - 1 | Boston City FCBoston City F |
| 17 May 23 |
r02        | FC Miami CityFC Miami City | 0 - 0 | Weston FCWeston FC |
| 18 May 23 |
r02        | Lansing City FootballLansing City | 1 - 0 | AFC Ann ArborAFC Ann Arbor |
| 18 May 23 |
r02        | Lehigh Valley UnitedLehigh Valley | 1 - 1 | Reading United ACReading Unite |
| 18 May 23 |
r02        | Real Central New JerseyReal Central | 1 - 2 | West Chester United IIWest Chester |
| 18 May 23 |
r02        | North Carolina Fusion U23North Carolin | 2 - 1 | Wake FCWake FC |
| 18 May 23 |
r02        | FC Motown IIFC Motown II | 4 - 1 | Morris Elite SCMorris Elite |
| 18 May 23 |
r02        | Southern California SeahoSouthern Cali | 1 - 1 | Capo FCCapo FC |
| 20 May 23 |
r02        | Kalamazoo FCKalamazoo FC | 0 - 2 | Flint City BucksFlint City Bu |
| 20 May 23 |
r02        | Kings Hammer FCKings Hammer | 2 - 1 | Cleveland Force SCCleveland For |
| 20 May 23 |
r02        | Wake FCWake FC | 0 - 3 | Tobacco Road FCTobacco Road |
| 20 May 23 |
r02        | Chicago City SCChicago City | 4 - 2 | Springfield ASCSpringfield A |
| 20 May 23 |
r02        | Boston BoltsBoston Bolts | 0 - 3 | Vermont Green FCVermont Green |
|
| |
| 20 May 23 |
r03        | Midwest United FCMidwest Unite | 5 - 1 | South Bend Lions FCSouth Bend Li |
|
| |
| 20 May 23 |
r02        | Philadelphia Lone Star FCPhiladelphia | 3 - 2 | Reading United ACReading Unite |
|
| |
| 20 May 23 |
r03        | Morris Elite SCMorris Elite | 1 - 1 | FA Euro New YorkFA Euro New Y |
| 21 May 23 |
r03        | Real Central New JerseyReal Central | 0 - 1 | Ocean City NoreastersOcean City No |
| 21 May 23 |
r03        | West Chester United IIWest Chester | 2 - 0 | Lehigh Valley UnitedLehigh Valley |
| 21 May 23 |
r03        | West Virginia UnitedWest Virginia | 2 - 2 | Charlotte EaglesCharlotte Eag |
| 21 May 23 |
r03        | Western Mass PioneersWestern Mass | 3 - 0 | FC Málaga City New YorkFC Málaga Cit |
| 21 May 23 |
r03        | Oakland County FCOakland Count | 1 - 1 | Lansing City FootballLansing City |
| 21 May 23 |
r03        | FC Motown IIFC Motown II | 1 - 3 | Hudson Valley HammersHudson Valley |
|
| |
| 21 May 23 |
r02        | Dalton Red Wolves SCDalton Red Wo | 0 - 1 | SC United BantamsSC United Ban |
| 21 May 23 |
r02        | Asheville City SCAsheville Cit | 2 - 0 | East Atlanta FCEast Atlanta |
| 21 May 23 |
r02        | Southern Soccer AcademySouthern Socc | 2 - 1 | Tennessee SCTennessee SC |
|
| |
| 21 May 23 |
r03        | FC WichitaFC Wichita | 1 - 3 | Des Moines MenaceDes Moines Me |
| 21 May 23 |
r03        | Long Island Rough RidersLong Island R | 2 - 0 | Westchester FlamesWestchester F |
| 21 May 23 |
r03        | Louisiana Krewe FCLouisiana Kre | 0 - 1 | Little Rock RangersLittle Rock R |
| 21 May 23 |
r03        | Blue Goose SCBlue Goose SC | 0 - 2 | LA Parish ACLA Parish AC |
| 21 May 23 |
r03        | Texas UnitedTexas United | 5 - 1 | Mississippi Brilla FCMississippi B |
| 21 May 23 |
r03        | Round Rock SCRound Rock SC | 1 - 3 | Houston FCHouston FC |
|
| |
| 21 May 23 |
r02        | Corpus Christi FCCorpus Christ | 4 - 1 | AC Houston SurAC Houston Su |
|
| |
| 21 May 23 |
r03        | St. Croix SCSt. Croix SC | 3 - 2 | Bavarian UnitedBavarian Unit |
| 21 May 23 |
r03        | Manhattan SCManhattan SC | 2 - 2 | Cedar Stars RushCedar Stars R |
| 21 May 23 |
r03        | Project 51OProject 51O | 3 - 2 | Marin FC LegendsMarin FC Lege |
|
| |
| 22 May 23 |
r04        | Lionsbridge FCLionsbridge F | 4 - 1 | Northern Virginia FCNorthern Virg |
| 22 May 23 |
r04        | Patuxent Football AthletiPatuxent Foot | 2 - 2 | Christos FCChristos FC |
| 23 May 23 |
r04        | North Carolina FC U23North Carolin | 0 - 4 | North Carolina Fusion U23North Carolin |
|
| |
| 24 May 23 |
r03        | Cleveland Force SCCleveland For | 2 - 2 | Fort Wayne FCFort Wayne FC |
|
| |
| 24 May 23 |
r04        | Charlotte Independence IICharlotte Ind | 3 - 2 | Wake FCWake FC |
|
| |
| 24 May 23 |
r03        | Springfield ASCSpringfield A | 1 - 0 | Chicago Dutch Lions FCChicago Dutch |
| 24 May 23 |
r03        | AHFC RoyalsAHFC Royals | 0 - 1 | AC Houston SurAC Houston Su |
| 24 May 23 |
r03        | Ventura County FusionVentura Count | 2 - 1 | Southern California SeahoSouthern Cali |
|
| |
| 25 May 23 |
r05        | Cedar Stars RushCedar Stars R | 3 - 2 | New Jersey Copa FCNew Jersey Co |
|
| |
| 25 May 23 |
r04        | Real Central New JerseyReal Central | 0 - 1 | Reading United ACReading Unite |
| 25 May 23 |
r04        | Lehigh Valley UnitedLehigh Valley | 1 - 3 | Ocean City NoreastersOcean City No |
| 25 May 23 |
r04        | Charlotte EaglesCharlotte Eag | 4 - 0 | Tobacco Road FCTobacco Road |
| 25 May 23 |
r04        | AC ConnecticutAC Connecticu | 0 - 1 | Black Rock FCBlack Rock FC |
|
| |
| 25 May 23 |
r03        | Midwest United FCMidwest Unite | 3 - 0 | Lansing City FootballLansing City |
|
| |
| 25 May 23 |
r05        | Patuxent Football AthletiPatuxent Foot | 0 - 2 | Lionsbridge FCLionsbridge F |
| 25 May 23 |
r05        | FA Euro New YorkFA Euro New Y | 0 - 2 | Long Island Rough RidersLong Island R |
| 26 May 23 |
r05        | Seacoast United PhantomsSeacoast Unit | 1 - 1 | Western Mass PioneersWestern Mass |
| 26 May 23 |
r05        | Charlotte Independence IICharlotte Ind | 0 - 1 | North Carolina FC U23North Carolin |
| 26 May 23 |
r05        | FC Málaga City New YorkFC Málaga Cit | 5 - 1 | Boston City FCBoston City F |
| 27 May 23 |
r05        | Ocean City NoreastersOcean City No | 1 - 0 | Philadelphia Lone Star FCPhiladelphia |
| 27 May 23 |
r05        | Vermont Green FCVermont Green | 3 - 0 | Albany RushAlbany Rush |
|
| |
| 27 May 23 |
r04        | Chicago Dutch Lions FCChicago Dutch | 0 - 5 | Des Moines MenaceDes Moines Me |
| 27 May 23 |
r04        | Ballard FCBallard FC | 3 - 0 | Lane United FCLane United F |
|
| |
| 27 May 23 |
r03        | Ventura County FusionVentura Count | 4 - 0 | FC TucsonFC Tucson |
|
| |
| 27 May 23 |
r04        | Ogden City SCOgden City SC | 0 - 0 | Park City Red Wolves SCPark City Red |
| 27 May 23 |
r04        | San Francisco GlensSan Francisco | 1 - 1 | Davis Legacy SCDavis Legacy |
|
| |
| 27 May 23 |
r06        | Black Rock FCBlack Rock FC | 1 - 1 | Boston BoltsBoston Bolts |
|
| |
| 28 May 23 |
r04        | South Bend Lions FCSouth Bend Li | 0 - 3 | Oakland County FCOakland Count |
| 28 May 23 |
r04        | Toledo Villa FCToledo Villa | 1 - 2 | Fort Wayne FCFort Wayne FC |
| 28 May 23 |
r04        | Cleveland Force SCCleveland For | 5 - 0 | Dayton Dutch LionsDayton Dutch |
|
| |
| 28 May 23 |
r06        | Virginia Beach United FCVirginia Beac | 2 - 0 | Patuxent Football AthletiPatuxent Foot |
| 28 May 23 |
r06        | Hudson Valley HammersHudson Valley | 0 - 3 | Long Island Rough RidersLong Island R |
| 28 May 23 |
r06        | Lehigh Valley UnitedLehigh Valley | 0 - 1 | Real Central New JerseyReal Central |
| 28 May 23 |
r06        | Reading United ACReading Unite | 2 - 2 | West Chester United IIWest Chester |
| 28 May 23 |
r06        | Charlotte EaglesCharlotte Eag | 3 - 4 | North Carolina Fusion U23North Carolin |
|
| |
| 28 May 23 |
r04        | Tampa Bay United SCTampa Bay Uni | 0 - 1 | The Villages SCThe Villages |
| 28 May 23 |
r04        | Flint City BucksFlint City Bu | 3 - 1 | Midwest United FCMidwest Unite |
|
| |
| 28 May 23 |
r06        | Northern Virginia FCNorthern Virg | 1 - 1 | Christos FCChristos FC |
| 28 May 23 |
r06        | Westchester FlamesWestchester F | 1 - 2 | Morris Elite SCMorris Elite |
|
| |
| 28 May 23 |
r04        | East Atlanta FCEast Atlanta | 0 - 1 | SC United BantamsSC United Ban |
| 28 May 23 |
r04        | Dalton Red Wolves SCDalton Red Wo | 0 - 1 | Southern Soccer AcademySouthern Socc |
| 28 May 23 |
r04        | FC ManitobaFC Manitoba | 1 - 1 | Thunder Bay ChillThunder Bay C |