| 06 May 21 |
r01        | West Chester United SCWest Chester | 1 - 0 | Philadelphia Lone Star FCPhiladelphia |
| 08 May 21 |
r01        | Tobacco Road FCTobacco Road | 0 - 5 | North Carolina Fusion U23North Carolin |
|
| |
| 09 May 21 |
r02        | Northern Virginia FCNorthern Virg | 3 - 2 | Philadelphia Lone Star FCPhiladelphia |
|
| |
| 09 May 21 |
r01        | Weston FCWeston FC | 4 - 2 | FC Miami CityFC Miami City |
|
| |
| 09 May 21 |
r02        | Oakland County FCOakland Count | 3 - 0 | Fort Wayne FCFort Wayne FC |
| 10 May 21 |
r02        | FC MotownFC Motown | 2 - 0 | Morris Elite SCMorris Elite |
| 12 May 21 |
r02        | Tormenta FC 2Tormenta FC 2 | 3 - 1 | Southern Soccer AcademySouthern Socc |
| 12 May 21 |
r02        | Virginia Beach United FCVirginia Beac | 0 - 0 | Lionsbridge FCLionsbridge F |
| 12 May 21 |
r02        | Boston BoltsBoston Bolts | 4 - 0 | Western Mass PioneersWestern Mass |
| 13 May 21 |
r02        | Real Central New JerseyReal Central | 0 - 3 | Ocean City NoreastersOcean City No |
| 13 May 21 |
r02        | West Chester United SCWest Chester | 5 - 0 | Lehigh Valley UnitedLehigh Valley |
| 13 May 21 |
r02        | Weston FCWeston FC | 0 - 0 | The Villages SCThe Villages |
| 13 May 21 |
r02        | Little Rock RangersLittle Rock R | 1 - 2 | Houston FCHouston FC |
|
| |
| 15 May 21 |
r03        | Tormenta FC 2Tormenta FC 2 | 4 - 0 | Asheville City SCAsheville Cit |
| 15 May 21 |
r03        | Charlotte Independence IICharlotte Ind | 1 - 1 | Charlotte EaglesCharlotte Eag |
| 15 May 21 |
r03        | Seacoast United PhantomsSeacoast Unit | 1 - 0 | Boston BoltsBoston Bolts |
| 15 May 21 |
r03        | North Carolina FC U23North Carolin | 1 - 7 | Lionsbridge FCLionsbridge F |
| 15 May 21 |
r03        | FC Florida U23FC Florida U2 | 0 - 2 | Florida Elite Soccer AcadFlorida Elite |
|
| |
| 16 May 21 |
r04        | Lehigh Valley UnitedLehigh Valley | 5 - 1 | Reading United ACReading Unite |
|
| |
| 16 May 21 |
r03        | West Chester United SCWest Chester | 0 - 0 | Real Central New JerseyReal Central |
| 16 May 21 |
r03        | Kalamazoo FCKalamazoo FC | 1 - 0 | South Bend Lions FCSouth Bend Li |
| 16 May 21 |
r03        | Dayton Dutch LionsDayton Dutch | 1 - 2 | Kings Hammer FCKings Hammer |
| 16 May 21 |
r03        | Wake FCWake FC | 0 - 2 | West Virginia UnitedWest Virginia |
|
| |
| 16 May 21 |
r04        | FC Málaga City New YorkFC Málaga Cit | 0 - 1 | Western Mass PioneersWestern Mass |
| 16 May 21 |
r04        | Flint City BucksFlint City Bu | 3 - 1 | Toledo Villa FCToledo Villa |
| 16 May 21 |
r04        | Mississippi Brilla FCMississippi B | 3 - 0 | Round Rock SCRound Rock SC |
| 16 May 21 |
r04        | Des Moines MenaceDes Moines Me | 5 - 1 | Green Bay Voyageurs FCGreen Bay Voy |
| 16 May 21 |
r04        | FC Miami CityFC Miami City | 1 - 1 | Tampa Bay United SCTampa Bay Uni |
| 17 May 21 |
r04        | Seacoast United PhantomsSeacoast Unit | 3 - 2 | AC ConnecticutAC Connecticu |
| 17 May 21 |
r04        | Grand Rapids FCGrand Rapids | 0 - 0 | Fort Wayne FCFort Wayne FC |
| 17 May 21 |
r04        | Corpus Christi FCCorpus Christ | 2 - 1 | Brazos Valley Cavalry FCBrazos Valley |
| 17 May 21 |
r04        | Cedar Stars RushCedar Stars R | 4 - 0 | Morris Elite SCMorris Elite |
|
| |
| 17 May 21 |
r05        | FC MotownFC Motown | 1 - 0 | New York Red Bulls U23New York Red |
| 17 May 21 |
r05        | Dalton Red Wolves SCDalton Red Wo | 1 - 2 | East Atlanta FCEast Atlanta |
| 19 May 21 |
r05        | Charlotte EaglesCharlotte Eag | 0 - 2 | Southern Soccer AcademySouthern Socc |
| 19 May 21 |
r05        | Virginia Beach United FCVirginia Beac | 2 - 2 | Wake FCWake FC |
| 19 May 21 |
r05        | North Carolina Fusion U23North Carolin | 3 - 1 | Tri-Cities OttersTri-Cities Ot |
| 19 May 21 |
r05        | East Atlanta FCEast Atlanta | 1 - 2 | SC United BantamsSC United Ban |
| 19 May 21 |
r05        | St. Louis Scott GallagherSt. Louis Sco | 0 - 1 | Des Moines MenaceDes Moines Me |
| 19 May 21 |
r05        | Toledo Villa FCToledo Villa | 5 - 2 | Dayton Dutch LionsDayton Dutch |
| 19 May 21 |
r05        | North Carolina FC U23North Carolin | 0 - 2 | West Virginia UnitedWest Virginia |
|
| |
| 20 May 21 |
r06        | Lehigh Valley UnitedLehigh Valley | 0 - 7 | Ocean City NoreastersOcean City No |
|
| |
| 20 May 21 |
r05        | Charlotte Independence IICharlotte Ind | 2 - 1 | Tormenta FC 2Tormenta FC 2 |
|
| |
| 20 May 21 |
r06        | Kings Hammer FCKings Hammer | 2 - 4 | Kalamazoo FCKalamazoo FC |
| 20 May 21 |
r06        | West Chester United SCWest Chester | 2 - 0 | Reading United ACReading Unite |
| 20 May 21 |
r06        | Weston FCWeston FC | 0 - 1 | Florida Elite Soccer AcadFlorida Elite |
| 20 May 21 |
r06        | Westchester FlamesWestchester F | 2 - 3 | FA Euro New YorkFA Euro New Y |
| 20 May 21 |
r06        | Dalton Red Wolves SCDalton Red Wo | 1 - 0 | Asheville City SCAsheville Cit |
| 20 May 21 |
r06        | Boston BoltsBoston Bolts | 1 - 0 | FC Málaga City New YorkFC Málaga Cit |
| 20 May 21 |
r06        | AHFC RoyalsAHFC Royals | 2 - 2 | Round Rock SCRound Rock SC |
| 21 May 21 |
r06        | St. Louis Scott GallagherSt. Louis Sco | 1 - 2 | Des Moines MenaceDes Moines Me |
| 22 May 21 |
r06        | Reading United ACReading Unite | 1 - 2 | Northern Virginia FCNorthern Virg |
|
| |
| 22 May 21 |
r07        | Western Mass PioneersWestern Mass | 1 - 1 | Seacoast United PhantomsSeacoast Unit |
| 22 May 21 |
r07        | Grand Rapids FCGrand Rapids | 1 - 1 | Kalamazoo FCKalamazoo FC |
| 22 May 21 |
r07        | Mississippi Brilla FCMississippi B | 5 - 2 | Houston FCHouston FC |
| 22 May 21 |
r07        | AC ConnecticutAC Connecticu | 1 - 3 | Boston BoltsBoston Bolts |
| 22 May 21 |
r07        | Philadelphia Lone Star FCPhiladelphia | 2 - 0 | Lehigh Valley UnitedLehigh Valley |
| 22 May 21 |
r07        | The Villages SCThe Villages | 3 - 0 | FC Miami CityFC Miami City |
|
| |
| 23 May 21 |
r08        | Charlotte Independence IICharlotte Ind | 2 - 3 | Dalton Red Wolves SCDalton Red Wo |
|
| |
| 23 May 21 |
r07        | Lionsbridge FCLionsbridge F | 4 - 1 | Wake FCWake FC |
| 23 May 21 |
r07        | North Carolina Fusion U23North Carolin | 4 - 0 | North Carolina FC U23North Carolin |
| 23 May 21 |
r07        | Tobacco Road FCTobacco Road | 1 - 0 | Virginia Beach United FCVirginia Beac |
| 23 May 21 |
r07        | Florida Elite Soccer AcadFlorida Elite | 1 - 0 | Tampa Bay United SCTampa Bay Uni |
|
| |
| 23 May 21 |
r08        | Oakland County FCOakland Count | 0 - 1 | Kings Hammer FCKings Hammer |
| 23 May 21 |
r08        | Dayton Dutch LionsDayton Dutch | 0 - 5 | South Bend Lions FCSouth Bend Li |
| 23 May 21 |
r08        | Westchester FlamesWestchester F | 1 - 2 | Cedar Stars RushCedar Stars R |
| 23 May 21 |
r08        | Asheville City SCAsheville Cit | 2 - 0 | Southern Soccer AcademySouthern Socc |
| 23 May 21 |
r08        | Peachtree City MOBAPeachtree Cit | 2 - 2 | SC United BantamsSC United Ban |
| 23 May 21 |
r08        | West Virginia UnitedWest Virginia | 1 - 0 | Tri-Cities OttersTri-Cities Ot |
|
| |
| 23 May 21 |
r09        | FC WichitaFC Wichita | 0 - 3 | Kaw Valley FCKaw Valley FC |
| 23 May 21 |
r09        | FA Euro New YorkFA Euro New Y | 2 - 2 | FC MotownFC Motown |
| 23 May 21 |
r09        | Long Island Rough RidersLong Island R | 2 - 0 | New Jersey Copa FCNew Jersey Co |
|
| |
| 23 May 21 |
r08        | Green Bay Voyageurs FCGreen Bay Voy | - | Peoria CityPeoria City |
| 23 May 21 |
r08        | AHFC RoyalsAHFC Royals | 3 - 2 | Little Rock RangersLittle Rock R |
| 23 May 21 |
r08        | Green Bay Voyageurs FCGreen Bay Voy | 1 - 3 | Chicago FC UnitedChicago FC Un |
|
| |
| 23 May 21 |
r09        | Fort Wayne FCFort Wayne FC | 1 - 2 | Toledo Villa FCToledo Villa |
| 23 May 21 |
r09        | FC Málaga City New YorkFC Málaga Cit | 1 - 1 | Seacoast United PhantomsSeacoast Unit |
| 24 May 21 |
r09        | Ocean City NoreastersOcean City No | 2 - 1 | West Chester United SCWest Chester |
| 24 May 21 |
r09        | Corpus Christi FCCorpus Christ | 3 - 1 | Texas UnitedTexas United |
| 25 May 21 |
r09        | St. Louis Scott GallagherSt. Louis Sco | 2 - 0 | FC WichitaFC Wichita |
|
| |
| 26 May 21 |
r10        | Real Central New JerseyReal Central | 3 - 2 | Philadelphia Lone Star FCPhiladelphia |
| 26 May 21 |
r10        | Charlotte Independence IICharlotte Ind | 0 - 0 | SC United BantamsSC United Ban |
| 26 May 21 |
r10        | East Atlanta FCEast Atlanta | 0 - 4 | Asheville City SCAsheville Cit |
| 26 May 21 |
r10        | Tormenta FC 2Tormenta FC 2 | 1 - 0 | Dalton Red Wolves SCDalton Red Wo |
|
| |
| 26 May 21 |
r09        | Tobacco Road FCTobacco Road | 3 - 3 | West Virginia UnitedWest Virginia |
| 26 May 21 |
r09        | Wake FCWake FC | 2 - 3 | Virginia Beach United FCVirginia Beac |
|
| |
| 26 May 21 |
r10        | Toledo Villa FCToledo Villa | 5 - 5 | Oakland County FCOakland Count |
| 26 May 21 |
r10        | Kings Hammer FCKings Hammer | 0 - 0 | Flint City BucksFlint City Bu |
| 26 May 21 |
r10        | Morris Elite SCMorris Elite | 1 - 0 | FA Euro New YorkFA Euro New Y |
| 26 May 21 |
r10        | Chicago FC UnitedChicago FC Un | - | Peoria CityPeoria City |
| 26 May 21 |
r10        | Northern Virginia FCNorthern Virg | 2 - 3 | West Chester United SCWest Chester |
|
| |
| 26 May 21 |
r11        | North Carolina FC U23North Carolin | 0 - 5 | Tri-Cities OttersTri-Cities Ot |
|
| |
| 26 May 21 |
r10        | FC Florida U23FC Florida U2 | 0 - 1 | The Villages SCThe Villages |
|
| |
| 27 May 21 |
r11        | North Carolina Fusion U23North Carolin | 2 - 1 | Lionsbridge FCLionsbridge F |
| 27 May 21 |
r11        | AC ConnecticutAC Connecticu | 0 - 2 | Western Mass PioneersWestern Mass |
| 27 May 21 |
r11        | Dayton Dutch LionsDayton Dutch | 0 - 3 | Kalamazoo FCKalamazoo FC |
| 27 May 21 |
r11        | Westchester FlamesWestchester F | 0 - 2 | Long Island Rough RidersLong Island R |
| 27 May 21 |
r11        | Morris Elite SCMorris Elite | 0 - 2 | New Jersey Copa FCNew Jersey Co |
| 27 May 21 |
r11        | Boston BoltsBoston Bolts | 1 - 0 | FC Málaga City New YorkFC Málaga Cit |