| 08 Sep 23 | r00  | Bowers and PitseaBowers and P | 3 - 1 | GorlestonGorleston |
| 08 Sep 23 | r00 | UxbridgeUxbridge | 1 - 2 | Sevenoaks TownSevenoaks Tow |
| 09 Sep 23 | r00 | RamsgateRamsgate | 3 - 0 | NorthwoodNorthwood |
| 09 Sep 23 | r00 | MersthamMerstham | 0 - 3 | Sutton Common RoversSutton Common |
| 09 Sep 23 | r00 | ChipsteadChipstead | 2 - 4 | LancingLancing |
| 09 Sep 23 | r00 | East Grinstead TownEast Grinstea | 0 - 1 | Sheppey UnitedSheppey Unite |
| 09 Sep 23 | r00 | Burgess Hill TownBurgess Hill | 1 - 3 | Hythe TownHythe Town |
| 09 Sep 23 | r00 | Phoenix SportsPhoenix Sport | 1 - 1 | Metropolitan Police FCMetropolitan |
| 09 Sep 23 | r00 | Westfield SurreyWestfield Sur | 4 - 1 | Littlehampton TownLittlehampton |
| 09 Sep 23 | r00 | SouthallSouthall | 2 - 1 | Chichester CityChichester Ci |
| 09 Sep 23 | r00 | Beckenham TownBeckenham Tow | 1 - 5 | Three BridgesThree Bridges |
| 09 Sep 23 | r00 | Biggleswade TownBiggleswade T | 2 - 3 | Bury TownBury Town |
| 09 Sep 23 | r00 | Heybridge SwiftsHeybridge Swi | 3 - 2 | Barton RoversBarton Rovers |
| 09 Sep 23 | r00 | WroxhamWroxham | 2 - 4 | Brightlingsea RegentBrightlingsea |
| 09 Sep 23 | r00 | Welwyn Garden CityWelwyn Garden | 3 - 5 | Basildon UnitedBasildon Unit |
| 09 Sep 23 | r00 | Kings LangleyKings Langley | 1 - 2 | Cambridge CityCambridge Cit |
| 09 Sep 23 | r00 | AylesburyAylesbury | 2 - 3 | Bedford TownBedford Town |
| 09 Sep 23 | r00 | Yate TownYate Town | 0 - 2 | TavistockTavistock |
| 09 Sep 23 | r00 | ExmouthExmouth | 3 - 3 | Wimborne TownWimborne Town |
| 09 Sep 23 | r00 | Thatcham TownThatcham Town | 2 - 2 | Bristol Manor FarmBristol Manor |
| 09 Sep 23 | r00 | Larkhall AthleticLarkhall Athl | 2 - 0 | Willand RoversWilland Rover |
| 09 Sep 23 | r00 | Bishops CleeveBishops Cleev | 6 - 1 | Paulton RoversPaulton Rover |
| 09 Sep 23 | r00 | Evesham UnitedEvesham Unite | 1 - 1 | BashleyBashley |
| 09 Sep 23 | r00 | KidlingtonKidlington | 2 - 0 | North LeighNorth Leigh |
| 09 Sep 23 | r00 | HadleyHadley | 4 - 1 | WareWare |
| 09 Sep 23 | r00 | Frome TownFrome Town | 3 - 1 | CirencesterCirencester |
| 09 Sep 23 | r00 | Hamworthy UnitedHamworthy Uni | 3 - 0 | Melksham TownMelksham Town |
| 09 Sep 23 | r00 | Corinthian-CasualsCorinthian-Ca | 0 - 1 | Hartley WintneyHartley Wintn |
| 09 Sep 23 | r00 | SittingbourneSittingbourne | 2 - 1 | Ashford Town MiddlesexAshford Town |
| 09 Sep 23 | r00 | Hanworth VillaHanworth Vill | 1 - 1 | Chertsey TownChertsey Town |
| 09 Sep 23 | r00 | Herne BayHerne Bay | 1 - 6 | LeatherheadLeatherhead |
| 09 Sep 23 | r00 | Belper TownBelper Town | 2 - 0 | Newcastle TownNewcastle Tow |
| 09 Sep 23 | r00 | Gresley RoversGresley Rover | 0 - 6 | Kidsgrove AthleticKidsgrove Ath |
| 09 Sep 23 | r00 | Bedworth UnitedBedworth Unit | 1 - 5 | Leek TownLeek Town |
| 09 Sep 23 | r00 | Sporting KhalsaSporting Khal | 1 - 0 | Grimsby BoroughGrimsby Borou |
| 09 Sep 23 | r00 | Stockton TownStockton Town | 3 - 1 | Pontefract CollieriesPontefract Co |
| 09 Sep 23 | r00 | Runcorn LinnetsRuncorn Linne | 0 - 0 | WittonWitton |
| 09 Sep 23 | r00 | BootleBootle | 6 - 2 | ConsettConsett |
| 09 Sep 23 | r00 | Sutton Coldfield TownSutton Coldfi | 0 - 2 | Boldmere St MichaelsBoldmere St M |
| 09 Sep 23 | r00 | Prescot CablesPrescot Cable | 4 - 0 | Bridlington TownBridlington T |
| 09 Sep 23 | r00 | TraffordTrafford | 1 - 2 | StalybridgeStalybridge |
| 09 Sep 23 | r00 | WidnesWidnes | 0 - 1 | ClitheroeClitheroe |
| 09 Sep 23 | r00 | LiversedgeLiversedge | 0 - 3 | Nantwich TownNantwich Town |
| 09 Sep 23 | r00 | BiggleswadeBiggleswade | 3 - 1 | Stowmarket TownStowmarket To |
| 09 Sep 23 | r00 | Lowestoft TownLowestoft Tow | 5 - 3 | New SalamisNew Salamis |
| 09 Sep 23 | r00 | Witham TownWitham Town | 3 - 0 | Maldon and TiptreeMaldon and Ti |
| 09 Sep 23 | r00 | AFC DunstableAFC Dunstable | - | East Thurrock UnitedEast Thurrock |
| 09 Sep 23 | r00 | Hertford TownHertford Town | 3 - 2 | GraysGrays |
| 09 Sep 23 | r00 | Felixstowe and Walton UniFelixstowe an | 2 - 0 | Brentwood TownBrentwood Tow |
| 09 Sep 23 | r00 | Brighouse TownBrighouse Tow | 1 - 1 | Hebburn TownHebburn Town |
| 09 Sep 23 | r00 | Coleshill TownColeshill Tow | 1 - 5 | Carlton TownCarlton Town |
| 09 Sep 23 | r00 | Loughborough DynamoLoughborough | 1 - 1 | AFC Rushden and DiamondsAFC Rushden a |
| 09 Sep 23 | r00 | ChasetownChasetown | 5 - 1 | Shepshed DynamoShepshed Dyna |
| 09 Sep 23 | r00 | Hanley TownHanley Town | 2 - 0 | Cleethorpes TownCleethorpes T |
| 09 Sep 23 | r00 | Sheffield FCSheffield FC | 1 - 1 | Spalding UnitedSpalding Unit |
| 09 Sep 23 | r00 | South ParkSouth Park | 2 - 1 | Erith and BelvedereErith and Bel |
| 09 Sep 23 | r00 | Raynes Park ValeRaynes Park V | 0 - 1 | MarlowMarlow |
| 09 Sep 23 | r00 | Thame UnitedThame United | 0 - 5 | Badshot LeaBadshot Lea |
| 09 Sep 23 | r00 | HorndeanHorndean | 2 - 3 | Ascot UnitedAscot United |
| 09 Sep 23 | r00 | Waltham AbbeyWaltham Abbey | 6 - 2 | Ipswich WanderersIpswich Wande |
| 09 Sep 23 | r00 | Malvern TownMalvern Town | 2 - 4 | MouseholeMousehole |
| 09 Sep 23 | r00 | CribbsCribbs | 3 - 2 | BidefordBideford |
| 09 Sep 23 | r00 | Westbury UnitedWestbury Unit | 2 - 5 | Bemerton Heath HarlequinsBemerton Heat |
| 09 Sep 23 | r00 | Broadbridge HeathBroadbridge H | 0 - 2 | Cray Valley PMCray Valley P |
| 09 Sep 23 | r00 | Dunston UTSDunston UTS | 3 - 0 | Ashington AFCAshington AFC |
| 09 Sep 23 | r00 | Anstey NomadsAnstey Nomads | 2 - 0 | Corby TownCorby Town |
| 09 Sep 23 | r00 | QuornQuorn | 4 - 0 | Coventry SphinxCoventry Sphi |
| 09 Sep 23 | r00 | Newton AycliffeNewton Ayclif | 1 - 3 | Stocksbridge Park SteelsStocksbridge |
| 09 Sep 23 | r00 | MossleyMossley | 0 - 2 | Vauxhall MotorsVauxhall Moto |
| 09 Sep 23 | r00 | AvroAvro | 2 - 1 | Winterton RangersWinterton Ran |
| 09 Sep 23 | r00 | North Ferriby FCNorth Ferriby | 2 - 1 | Ossett UnitedOssett United |
| 09 Sep 23 | r00 | Leighton TownLeighton Town | 3 - 1 | AFC Kempston RoversAFC Kempston |
| 09 Sep 23 | r00 | RedbridgeRedbridge | 1 - 0 | StotfoldStotfold |
| 09 Sep 23 | r00 | Harborough TownHarborough To | 2 - 1 | Lye TownLye Town |
| 09 Sep 23 | r00 | Hednesford TownHednesford To | 0 - 3 | Walsall WoodWalsall Wood |
| 09 Sep 23 | r00 | Rugby TownRugby Town | 1 - 5 | Grantham TownGrantham Town |
| 10 Sep 23 | r00 | Enfield FCEnfield FC | - | WalthamstowWalthamstow |
| 10 Sep 23 | r00 | Ashford UnitedAshford Unite | 7 - 1 | BinfieldBinfield |
| 10 Sep 23 | r00 | City of LiverpoolCity of Liver | 3 - 1 | 1874 Northwich1874 Northwic |
| 23 Sep 23 | r00 | AvroAvro | 0 - 0 | StalybridgeStalybridge |
| 23 Sep 23 | r00 | Nantwich TownNantwich Town | 2 - 1 | Dunston UTSDunston UTS |
| 23 Sep 23 | r00 | Stockton TownStockton Town | 0 - 2 | BootleBootle |
| 23 Sep 23 | r00 | Vauxhall MotorsVauxhall Moto | 0 - 1 | Stocksbridge Park SteelsStocksbridge |
| 23 Sep 23 | r00 | Brighouse TownBrighouse Tow | 2 - 2 | ClitheroeClitheroe |
| 23 Sep 23 | r00 | North Ferriby FCNorth Ferriby | 2 - 2 | Prescot CablesPrescot Cable |
| 23 Sep 23 | r00 | Hanley TownHanley Town | 2 - 1 | Sheffield FCSheffield FC |
| 23 Sep 23 | r00 | Kidsgrove AthleticKidsgrove Ath | 2 - 4 | Sporting KhalsaSporting Khal |
| 23 Sep 23 | r00 | QuornQuorn | 4 - 2 | Leek TownLeek Town |
| 23 Sep 23 | r00 | Belper TownBelper Town | 1 - 2 | Walsall WoodWalsall Wood |
| 23 Sep 23 | r00 | Boldmere St MichaelsBoldmere St M | 2 - 1 | Grantham TownGrantham Town |
| 23 Sep 23 | r00 | Carlton TownCarlton Town | 2 - 1 | ChasetownChasetown |
| 23 Sep 23 | r00 | Anstey NomadsAnstey Nomads | 2 - 2 | Loughborough DynamoLoughborough |
| 23 Sep 23 | r00 | Hinckley Leicester RoadHinckley Leic | 0 - 0 | Harborough TownHarborough To |
| 23 Sep 23 | r00 | Felixstowe and Walton UniFelixstowe an | 6 - 3 | Hertford TownHertford Town |
| 23 Sep 23 | r00 | BiggleswadeBiggleswade | 2 - 1 | HadleyHadley |
| 23 Sep 23 | r00 | AFC DunstableAFC Dunstable | 2 - 3 | Brightlingsea RegentBrightlingsea |
| 23 Sep 23 | r00 | Witham TownWitham Town | 2 - 3 | RedbridgeRedbridge |
| 23 Sep 23 | r00 | Waltham AbbeyWaltham Abbey | 1 - 5 | Heybridge SwiftsHeybridge Swi |
| 23 Sep 23 | r00 | Basildon UnitedBasildon Unit | 0 - 4 | Lowestoft TownLowestoft Tow |
| 23 Sep 23 | r00 | South ParkSouth Park | 2 - 1 | Three BridgesThree Bridges |
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