| 12 Sep 22 | Qualifier  | MersthamMerstham | 3 - 0 | Hythe TownHythe Town |
| 12 Sep 22 | Qualifier | Brighouse TownBrighouse Tow | 0 - 2 | Worksop TownWorksop Town |
| 12 Sep 22 | Qualifier | Grantham TownGrantham Town | 1 - 2 | Sutton Coldfield TownSutton Coldfi |
| 13 Sep 22 | Qualifier | Willand RoversWilland Rover | 0 - 3 | BidefordBideford |
| 13 Sep 22 | Qualifier | Ashford UnitedAshford Unite | 3 - 3 | AFC SudburyAFC Sudbury |
| 13 Sep 22 | Qualifier | Maldon & TiptreeMaldon & Tipt | 0 - 3 | WareWare |
| 13 Sep 22 | Qualifier | FC RomaniaFC Romania | 1 - 1 | Westfield SurreyWestfield Sur |
| 13 Sep 22 | Qualifier | Three BridgesThree Bridges | 3 - 2 | Hullbridge SportsHullbridge Sp |
| 13 Sep 22 | Qualifier | Walton & HershamWalton & Hers | 3 - 1 | LeatherheadLeatherhead |
| 13 Sep 22 | Qualifier | Great Wakering RoversGreat Wakerin | 1 - 2 | Harlow TownHarlow Town |
| 13 Sep 22 | Qualifier | VCD AthleticVCD Athletic | 0 - 3 | GraysGrays |
| 13 Sep 22 | Qualifier | Tooting & Mitcham UnitedTooting & Mit | 0 - 0 | CorinthianCorinthian |
| 13 Sep 22 | Qualifier | Witham TownWitham Town | 1 - 0 | Brentwood TownBrentwood Tow |
| 13 Sep 22 | Qualifier | Felixstowe & Walton UniteFelixstowe & | 2 - 2 | Burgess Hill TownBurgess Hill |
| 13 Sep 22 | Qualifier | TilburyTilbury | 1 - 2 | WhitehawkWhitehawk |
| 13 Sep 22 | Qualifier | LancingLancing | 1 - 4 | Cray Valley PMCray Valley P |
| 13 Sep 22 | Qualifier | WroxhamWroxham | 4 - 1 | East Grinstead TownEast Grinstea |
| 13 Sep 22 | Qualifier | Welwyn Garden CityWelwyn Garden | 1 - 2 | New SalamisNew Salamis |
| 13 Sep 22 | Qualifier | Bedfont SportsBedfont Sport | 2 - 2 | Hashtag UnitedHashtag Unite |
| 13 Sep 22 | Qualifier | Barton RoversBarton Rovers | 0 - 1 | Chatham TownChatham Town |
| 13 Sep 22 | Qualifier | AFC TottonAFC Totton | 1 - 1 | SlimbridgeSlimbridge |
| 13 Sep 22 | Qualifier | Highworth TownHighworth Tow | 1 - 2 | Paulton RoversPaulton Rover |
| 13 Sep 22 | Qualifier | Wimborne TownWimborne Town | 2 - 3 | Bristol Manor FarmBristol Manor |
| 13 Sep 22 | Qualifier | Thame UnitedThame United | 0 - 2 | Westbury UnitedWestbury Unit |
| 13 Sep 22 | Qualifier | KidlingtonKidlington | 0 - 0 | TavistockTavistock |
| 13 Sep 22 | Qualifier | MarlowMarlow | 0 - 4 | BinfieldBinfield |
| 13 Sep 22 | Qualifier | BashleyBashley | 4 - 1 | Frome TownFrome Town |
| 13 Sep 22 | Qualifier | BerkhamstedBerkhamsted | 5 - 0 | ExmouthExmouth |
| 13 Sep 22 | Qualifier | BasingstokeBasingstoke | 3 - 5 | CirencesterCirencester |
| 13 Sep 22 | Qualifier | Bishops CleeveBishops Cleev | 2 - 3 | AFC DunstableAFC Dunstable |
| 13 Sep 22 | Qualifier | WalthamstowWalthamstow | 1 - 3 | Ashford Town MiddlesexAshford Town |
| 13 Sep 22 | Qualifier | SholingSholing | 0 - 1 | Lymington TownLymington Tow |
| 13 Sep 22 | Qualifier | Didcot TownDidcot Town | 1 - 1 | Hamworthy UnitedHamworthy Uni |
| 13 Sep 22 | Qualifier | Cinderford TownCinderford To | 0 - 1 | Thatcham TownThatcham Town |
| 13 Sep 22 | Qualifier | Littlehampton TownLittlehampton | 1 - 0 | RamsgateRamsgate |
| 13 Sep 22 | Qualifier | Haywards Heath TownHaywards Heat | 0 - 0 | SouthallSouthall |
| 13 Sep 22 | Qualifier | Chichester CityChichester Ci | 0 - 1 | Faversham TownFaversham Tow |
| 13 Sep 22 | Qualifier | Stamford AFCStamford AFC | 1 - 2 | ChasetownChasetown |
| 13 Sep 22 | Qualifier | DaventryDaventry | 2 - 3 | Lincoln UnitedLincoln Unite |
| 13 Sep 22 | Qualifier | Newcastle TownNewcastle Tow | 0 - 2 | Spalding UnitedSpalding Unit |
| 13 Sep 22 | Qualifier | Bedworth UnitedBedworth Unit | 0 - 1 | Harborough TownHarborough To |
| 13 Sep 22 | Qualifier | Bridlington TownBridlington T | 1 - 2 | ColneColne |
| 13 Sep 22 | Qualifier | North ShieldsNorth Shields | 3 - 1 | Pontefract CollieriesPontefract Co |
| 13 Sep 22 | Qualifier | WorkingtonWorkington | 1 - 2 | Runcorn LinnetsRuncorn Linne |
| 13 Sep 22 | Qualifier | MossleyMossley | 1 - 2 | Dunston UTSDunston UTS |
| 13 Sep 22 | Qualifier | Prescot CablesPrescot Cable | 1 - 1 | Cleethorpes TownCleethorpes T |
| 13 Sep 22 | Qualifier | ClitheroeClitheroe | 2 - 1 | 1874 Northwich1874 Northwic |
| 13 Sep 22 | Qualifier | BootleBootle | - | Grimsby BoroughGrimsby Borou |
| 13 Sep 22 | Qualifier | WittonWitton | 3 - 4 | Stockton TownStockton Town |
| 13 Sep 22 | Qualifier | Stocksbridge Park SteelsStocksbridge | 2 - 2 | Ossett UnitedOssett United |
| 13 Sep 22 | Qualifier | MacclesfieldMacclesfield | 7 - 0 | Tadcaster AlbionTadcaster Alb |
| 13 Sep 22 | Qualifier | Shildon AFCShildon AFC | 1 - 2 | ConsettConsett |
| 13 Sep 22 | Qualifier | Sheppey UnitedSheppey Unite | 3 - 3 | Hertford TownHertford Town |
| 13 Sep 22 | Qualifier | Bury TownBury Town | 1 - 2 | Stowmarket TownStowmarket To |
| 13 Sep 22 | Qualifier | East Thurrock UnitedEast Thurrock | 3 - 1 | Sutton Common RoversSutton Common |
| 13 Sep 22 | Qualifier | Beckenham TownBeckenham Tow | 1 - 1 | Heybridge SwiftsHeybridge Swi |
| 13 Sep 22 | Qualifier | Chertsey TownChertsey Town | 1 - 3 | UxbridgeUxbridge |
| 13 Sep 22 | Qualifier | ChipsteadChipstead | 4 - 2 | Waltham AbbeyWaltham Abbey |
| 13 Sep 22 | Qualifier | NorthwoodNorthwood | 0 - 3 | Hanworth VillaHanworth Vill |
| 13 Sep 22 | Qualifier | Basildon UnitedBasildon Unit | 0 - 2 | Lowestoft TownLowestoft Tow |
| 13 Sep 22 | Qualifier | AFC Kempston RoversAFC Kempston | 0 - 1 | Dereham TownDereham Town |
| 13 Sep 22 | Qualifier | Ramsbottom UnitedRamsbottom Un | 2 - 1 | Glossop North EndGlossop North |
| 13 Sep 22 | Qualifier | Kidsgrove AthleticKidsgrove Ath | 3 - 4 | Hinckley Leicester RoadHinckley Leic |
| 13 Sep 22 | Qualifier | Halesowen TownHalesowen Tow | 5 - 2 | BiggleswadeBiggleswade |
| 13 Sep 22 | Qualifier | Boldmere St MichaelsBoldmere St M | 4 - 3 | Sporting KhalsaSporting Khal |
| 13 Sep 22 | Qualifier | Loughborough DynamoLoughborough | 3 - 1 | Corby TownCorby Town |
| 13 Sep 22 | Qualifier | Coleshill TownColeshill Tow | 3 - 2 | St Neots TownSt Neots Town |
| 13 Sep 22 | Qualifier | Shepshed DynamoShepshed Dyna | 1 - 0 | Biggleswade TownBiggleswade T |
| 13 Sep 22 | Qualifier | Long Eaton UnitedLong Eaton Un | 2 - 0 | Gresley RoversGresley Rover |
| 13 Sep 22 | Qualifier | Carlton TownCarlton Town | 2 - 0 | Hanley TownHanley Town |
| 13 Sep 22 | Qualifier | TraffordTrafford | 1 - 2 | Hebburn TownHebburn Town |
| 14 Sep 22 | Qualifier | HadleyHadley | 1 - 2 | Sevenoaks TownSevenoaks Tow |
| 14 Sep 22 | Qualifier | South ParkSouth Park | 1 - 2 | SittingbourneSittingbourne |
| 14 Sep 22 | Qualifier | Larkhall AthleticLarkhall Athl | 0 - 2 | Melksham TownMelksham Town |
| 14 Sep 22 | Qualifier | AylesburyAylesbury | 0 - 3 | Evesham UnitedEvesham Unite |
| 14 Sep 22 | Qualifier | Cambridge CityCambridge Cit | 4 - 1 | YaxleyYaxley |
| 14 Sep 22 | Qualifier | WidnesWidnes | 1 - 1 | Skelmersdale UnitedSkelmersdale |
| 14 Sep 22 | Qualifier | City of LiverpoolCity of Liver | 0 - 0 | Sheffield FCSheffield FC |
| 20 Sep 22 | Qualifier | GorlestonGorleston | 1 - 1 | Coggeshall TownCoggeshall To |
| |
| 23 Sep 22 | r00 | Hebburn TownHebburn Town | 3 - 1 | Stockton TownStockton Town |
| 24 Sep 22 | r00 | Prescot CablesPrescot Cable | 1 - 3 | Worksop TownWorksop Town |
| 24 Sep 22 | r00 | BootleBootle | 0 - 3 | Dunston UTSDunston UTS |
| 24 Sep 22 | r00 | ColneColne | 1 - 0 | ConsettConsett |
| 24 Sep 22 | r00 | Ramsbottom UnitedRamsbottom Un | 1 - 4 | ClitheroeClitheroe |
| 24 Sep 22 | r00 | North ShieldsNorth Shields | 1 - 2 | Runcorn LinnetsRuncorn Linne |
| 24 Sep 22 | r00 | Coleshill TownColeshill Tow | 4 - 4 | Leek TownLeek Town |
| 24 Sep 22 | r00 | Long Eaton UnitedLong Eaton Un | 0 - 0 | Loughborough DynamoLoughborough |
| 24 Sep 22 | r00 | Dereham TownDereham Town | 0 - 1 | Spalding UnitedSpalding Unit |
| 24 Sep 22 | r00 | Harborough TownHarborough To | 2 - 1 | Shepshed DynamoShepshed Dyna |
| 24 Sep 22 | r00 | Lincoln UnitedLincoln Unite | 1 - 1 | Carlton TownCarlton Town |
| 24 Sep 22 | r00 | Sutton Coldfield TownSutton Coldfi | 2 - 0 | BiggleswadeBiggleswade |
| 24 Sep 22 | r00 | Boldmere St MichaelsBoldmere St M | 1 - 1 | ChasetownChasetown |
| 24 Sep 22 | r00 | Cray Valley PMCray Valley P | 5 - 3 | East Thurrock UnitedEast Thurrock |
| 24 Sep 22 | r00 | Chatham TownChatham Town | 0 - 1 | Stowmarket TownStowmarket To |
| 24 Sep 22 | r00 | Hanworth VillaHanworth Vill | 3 - 2 | Littlehampton TownLittlehampton |
| 24 Sep 22 | r00 | UxbridgeUxbridge | 2 - 0 | Bedfont SportsBedfont Sport |
| 24 Sep 22 | r00 | WroxhamWroxham | 5 - 1 | Lowestoft TownLowestoft Tow |
| 24 Sep 22 | r00 | CorinthianCorinthian | 2 - 4 | WhitehawkWhitehawk |
| 24 Sep 22 | r00 | Ashford UnitedAshford Unite | 2 - 0 | Three BridgesThree Bridges |
| 24 Sep 22 | r00 | Heybridge SwiftsHeybridge Swi | 2 - 1 | ChipsteadChipstead |
| 24 Sep 22 | r00 | Harlow TownHarlow Town | 4 - 1 | Ashford Town MiddlesexAshford Town |
| 24 Sep 22 | r00 | Walton & HershamWalton & Hers | 4 - 0 | SouthallSouthall |
| 24 Sep 22 | r00 | Witham TownWitham Town | 2 - 1 | WareWare |
| 24 Sep 22 | r00 | TavistockTavistock | 1 - 0 | Didcot TownDidcot Town |
| 24 Sep 22 | r00 | CirencesterCirencester | 3 - 2 | Paulton RoversPaulton Rover |
| 24 Sep 22 | r00 | AFC TottonAFC Totton | 2 - 1 | BerkhamstedBerkhamsted |
| 24 Sep 22 | r00 | Bristol Manor FarmBristol Manor | 2 - 2 | BidefordBideford |
| 24 Sep 22 | r00 | Thatcham TownThatcham Town | 0 - 3 | AFC DunstableAFC Dunstable |
| 24 Sep 22 | r00 | Thame UnitedThame United | 1 - 2 | BinfieldBinfield |
| 24 Sep 22 | r00 | MacclesfieldMacclesfield | 5 - 1 | WidnesWidnes |
| 24 Sep 22 | r00 | Sheffield FCSheffield FC | 1 - 1 | Ossett UnitedOssett United |
| 24 Sep 22 | r00 | Hinckley Leicester RoadHinckley Leic | 0 - 0 | Cambridge CityCambridge Cit |
| 24 Sep 22 | r00 | SittingbourneSittingbourne | 1 - 1 | Burgess Hill TownBurgess Hill |
| 24 Sep 22 | r00 | Sevenoaks TownSevenoaks Tow | 2 - 2 | Hertford TownHertford Town |
| 24 Sep 22 | r00 | Evesham UnitedEvesham Unite | 1 - 0 | Lymington TownLymington Tow |
| 24 Sep 22 | r00 | BashleyBashley | 2 - 2 | Melksham TownMelksham Town |
| 24 Sep 22 | r00 | New SalamisNew Salamis | 3 - 0 | Coggeshall TownCoggeshall To |
| 25 Sep 22 | r00 | GraysGrays | 4 - 1 | MersthamMerstham |
| 25 Sep 22 | r00 | FC RomaniaFC Romania | 2 - 3 | Faversham TownFaversham Tow |
| 08 Oct 22 | r00 | Nantwich TownNantwich Town | 0 - 2 | Warrington RylandsWarrington Ry |
| 08 Oct 22 | r00 | MarineMarine | 1 - 1 | RadcliffeRadcliffe |
| 08 Oct 22 | r00 | Hebburn TownHebburn Town | 1 - 2 | Warrington TownWarrington To |
| 08 Oct 22 | r00 | AshtonAshton | 0 - 0 | Dunston UTSDunston UTS |
| 08 Oct 22 | r00 | MacclesfieldMacclesfield | 6 - 1 | Bamber BridgeBamber Bridge |
| 08 Oct 22 | r00 | GuiseleyGuiseley | 3 - 1 | Whitby TownWhitby Town |
| 08 Oct 22 | r00 | Morpeth TownMorpeth Town | 2 - 3 | Lancaster CityLancaster Cit |
| 08 Oct 22 | r00 | FC United of ManchesterFC United of | 0 - 1 | LiversedgeLiversedge |
| 08 Oct 22 | r00 | StalybridgeStalybridge | 1 - 2 | South ShieldsSouth Shields |
| 08 Oct 22 | r00 | ClitheroeClitheroe | 2 - 2 | Marske UnitedMarske United |
| 08 Oct 22 | r00 | Atherton CollieriesAtherton Coll | 0 - 3 | Leek TownLeek Town |
| 08 Oct 22 | r00 | Stratford TownStratford Tow | 1 - 2 | Royston TownRoyston Town |
| 08 Oct 22 | r00 | LeistonLeiston | 1 - 0 | Evesham UnitedEvesham Unite |
| 08 Oct 22 | r00 | GainsboroughGainsborough | 3 - 1 | Barwell FCBarwell FC |
| 08 Oct 22 | r00 | Loughborough DynamoLoughborough | 1 - 2 | Worksop TownWorksop Town |
| 08 Oct 22 | r00 | Coalville TownCoalville Tow | 4 - 1 | Ilkeston TownIlkeston Town |
| 08 Oct 22 | r00 | Needham MarketNeedham Marke | 0 - 1 | StourbridgeStourbridge |
| 08 Oct 22 | r00 | Hitchin TownHitchin Town | 0 - 3 | RedditchRedditch |
| 08 Oct 22 | r00 | Spalding UnitedSpalding Unit | 1 - 2 | Stafford RangersStafford Rang |
| 08 Oct 22 | r00 | Belper TownBelper Town | 1 - 1 | St Ives TownSt Ives Town |
| 08 Oct 22 | r00 | AFC Rushden & DiamondsAFC Rushden & | 0 - 4 | TamworthTamworth |
| 08 Oct 22 | r00 | Bromsgrove SportingBromsgrove Sp | 0 - 3 | ChasetownChasetown |
| 08 Oct 22 | r00 | MatlockMatlock | 3 - 0 | Sutton Coldfield TownSutton Coldfi |
| 08 Oct 22 | r00 | Stowmarket TownStowmarket To | 1 - 1 | Mickleover SportsMickleover Sp |
| 08 Oct 22 | r00 | Basford Utd.Basford Utd. | 1 - 1 | Harborough TownHarborough To |
| 08 Oct 22 | r00 | Bedford TownBedford Town | 0 - 3 | AlvechurchAlvechurch |
| 08 Oct 22 | r00 | Rushall OlympicRushall Olymp | 3 - 4 | Hednesford TownHednesford To |
| 08 Oct 22 | r00 | Carshalton AthleticCarshalton At | 2 - 2 | Cray Valley PMCray Valley P |
| 08 Oct 22 | r00 | Harlow TownHarlow Town | 2 - 2 | HornchurchHornchurch |
| 08 Oct 22 | r00 | Hayes & Yeading UnitedHayes & Yeadi | 1 - 0 | New SalamisNew Salamis |
| 08 Oct 22 | r00 | Burgess Hill TownBurgess Hill | 2 - 1 | Hanworth VillaHanworth Vill |
| 08 Oct 22 | r00 | HendonHendon | 0 - 0 | BinfieldBinfield |
| 08 Oct 22 | r00 | Haringey BoroughHaringey Boro | 1 - 0 | Brightlingsea RegentBrightlingsea |
| 08 Oct 22 | r00 | UxbridgeUxbridge | 1 - 0 | Walton & HershamWalton & Hers |
| 08 Oct 22 | r00 | Ashford UnitedAshford Unite | 1 - 2 | Kings LangleyKings Langley |
| 08 Oct 22 | r00 | Hanwell TownHanwell Town | 3 - 1 | Cray WanderersCray Wanderer |
| 08 Oct 22 | r00 | GraysGrays | 0 - 0 | Faversham TownFaversham Tow |
| 08 Oct 22 | r00 | HorshamHorsham | 4 - 3 | AveleyAveley |
| 08 Oct 22 | r00 | BillericayBillericay | 2 - 0 | MargateMargate |
| 08 Oct 22 | r00 | Beaconsfield TownBeaconsfield | 2 - 2 | Hartley WintneyHartley Wintn |
| 08 Oct 22 | r00 | Metropolitan Police FCMetropolitan | 0 - 0 | Hastings UnitedHastings Unit |
| 08 Oct 22 | r00 | Witham TownWitham Town | 0 - 1 | AFC DunstableAFC Dunstable |
| 08 Oct 22 | r00 | LewesLewes | 1 - 0 | Sevenoaks TownSevenoaks Tow |
| 08 Oct 22 | r00 | Corinthian-CasualsCorinthian-Ca | 2 - 2 | Harrow BoroughHarrow Boroug |
| 08 Oct 22 | r00 | Herne BayHerne Bay | 1 - 2 | Bishops StortfordBishops Stort |
| 08 Oct 22 | r00 | Enfield TownEnfield Town | 0 - 4 | Chesham UnitedChesham Unite |
| 08 Oct 22 | r00 | Potters Bar TownPotters Bar T | 0 - 2 | Heybridge SwiftsHeybridge Swi |
| 08 Oct 22 | r00 | Folkestone InvictaFolkestone In | 1 - 1 | WhitehawkWhitehawk |
| 08 Oct 22 | r00 | KingstonianKingstonian | 2 - 2 | Wingate & FinchleyWingate & Fin |
| 08 Oct 22 | r00 | Bognor Regis TownBognor Regis | 1 - 0 | Bowers & PitseaBowers & Pits |
| 08 Oct 22 | r00 | CanveyCanvey | 1 - 4 | Bracknell TownBracknell Tow |
| 08 Oct 22 | r00 | Plymouth ParkwayPlymouth Park | 2 - 0 | Dorchester TownDorchester To |
| 08 Oct 22 | r00 | Merthyr TownMerthyr Town | 0 - 1 | AFC TottonAFC Totton |
| 08 Oct 22 | r00 | Yate TownYate Town | 0 - 2 | Weston-super-Mare FCWeston-super- |
| 08 Oct 22 | r00 | TavistockTavistock | 3 - 1 | Melksham TownMelksham Town |
| 08 Oct 22 | r00 | Swindon SupermarineSwindon Super | 3 - 0 | CirencesterCirencester |
| 08 Oct 22 | r00 | Bristol Manor FarmBristol Manor | 2 - 0 | Gosport BoroughGosport Borou |
| 08 Oct 22 | r00 | North LeighNorth Leigh | 3 - 3 | Tiverton TownTiverton Town |
| 08 Oct 22 | r00 | Winchester CityWinchester Ci | 2 - 0 | Poole Town F.CPoole Town F. |
| 08 Oct 22 | r00 | Hyde UnitedHyde United | 2 - 3 | ColneColne |
| 08 Oct 22 | r00 | Runcorn LinnetsRuncorn Linne | 2 - 1 | Ossett UnitedOssett United |
| 08 Oct 22 | r00 | Lincoln UnitedLincoln Unite | 1 - 3 | WroxhamWroxham |
| 08 Oct 22 | r00 | Nuneaton TownNuneaton Town | 2 - 0 | Hinckley Leicester RoadHinckley Leic |
| 09 Oct 22 | r00 | Truro CityTruro City | 1 - 0 | SalisburySalisbury |
| 29 Oct 22 | r00 | MacclesfieldMacclesfield | 0 - 2 | GuiseleyGuiseley |
| 29 Oct 22 | r00 | MatlockMatlock | 1 - 0 | Stafford RangersStafford Rang |
| 29 Oct 22 | r00 | Leek TownLeek Town | 2 - 1 | Worksop TownWorksop Town |
| 29 Oct 22 | r00 | Lancaster CityLancaster Cit | 3 - 2 | GainsboroughGainsborough |
| 29 Oct 22 | r00 | Warrington TownWarrington To | 3 - 1 | South ShieldsSouth Shields |
| 29 Oct 22 | r00 | LiversedgeLiversedge | 0 - 2 | TamworthTamworth |
| 29 Oct 22 | r00 | Basford Utd.Basford Utd. | 1 - 2 | MarineMarine |
| 29 Oct 22 | r00 | Hednesford TownHednesford To | 4 - 2 | StourbridgeStourbridge |
| 29 Oct 22 | r00 | ColneColne | 5 - 0 | Warrington RylandsWarrington Ry |
| 29 Oct 22 | r00 | Dunston UTSDunston UTS | 1 - 3 | Coalville TownCoalville Tow |
| 29 Oct 22 | r00 | ChasetownChasetown | 0 - 2 | Marske UnitedMarske United |
| 29 Oct 22 | r00 | Nuneaton TownNuneaton Town | 1 - 2 | AlvechurchAlvechurch |
| 29 Oct 22 | r00 | Royston TownRoyston Town | 0 - 2 | Heybridge SwiftsHeybridge Swi |
| 29 Oct 22 | r00 | AFC DunstableAFC Dunstable | 0 - 1 | Hanwell TownHanwell Town |
| 29 Oct 22 | r00 | UxbridgeUxbridge | 4 - 2 | Wingate and FinchleyWingate and |
| 29 Oct 22 | r00 | Chesham UnitedChesham Unite | 2 - 2 | LewesLewes |
| 29 Oct 22 | r00 | Harlow TownHarlow Town | 0 - 4 | Bishops StortfordBishops Stort |
| 29 Oct 22 | r00 | GraysGrays | 0 - 0 | Stowmarket TownStowmarket To |
| 29 Oct 22 | r00 | Haringey BoroughHaringey Boro | 1 - 3 | BillericayBillericay |
| 29 Oct 22 | r00 | LeistonLeiston | 2 - 1 | WroxhamWroxham |
| 29 Oct 22 | r00 | Carshalton AthleticCarshalton At | 0 - 1 | Kings LangleyKings Langley |
| 29 Oct 22 | r00 | Harrow BoroughHarrow Boroug | 2 - 0 | Hayes and Yeading UnitedHayes and Ye |
| 29 Oct 22 | r00 | Folkestone InvictaFolkestone In | 2 - 0 | HorshamHorsham |
| 29 Oct 22 | r00 | Plymouth ParkwayPlymouth Park | 3 - 1 | Bristol Manor FarmBristol Manor |
| 29 Oct 22 | r00 | TavistockTavistock | 2 - 1 | BinfieldBinfield |
| 29 Oct 22 | r00 | RedditchRedditch | 3 - 1 | AFC TottonAFC Totton |
| 29 Oct 22 | r00 | Weston-super-Mare FCWeston-super- | 1 - 2 | Bognor Regis TownBognor Regis |
| 29 Oct 22 | r00 | Winchester CityWinchester Ci | 0 - 5 | Swindon SupermarineSwindon Super |
| 29 Oct 22 | r00 | Beaconsfield TownBeaconsfield | 2 - 0 | Truro CityTruro City |
| 29 Oct 22 | r00 | Runcorn LinnetsRuncorn Linne | 3 - 2 | Belper TownBelper Town |
| 29 Oct 22 | r00 | Hastings UnitedHastings Unit | 4 - 2 | Burgess Hill TownBurgess Hill |
| 30 Oct 22 | r00 | Bracknell TownBracknell Tow | 3 - 2 | North LeighNorth Leigh |
| 19 Nov 22 | r00 | Ebbsfleet UnitedEbbsfleet Uni | 0 - 1 | Eastbourne BoroughEastbourne Bo |
| 19 Nov 22 | r00 | Bradford Park AvenueBradford Park | 1 - 2 | Farsley CelticFarsley Celti |
| 19 Nov 22 | r00 | ChorleyChorley | 1 - 0 | Leek TownLeek Town |
| 19 Nov 22 | r00 | Chester CityChester City | 2 - 1 | Lancaster CityLancaster Cit |
| 19 Nov 22 | r00 | Spennymoor Town FCSpennymoor To | 3 - 2 | Runcorn LinnetsRuncorn Linne |
| 19 Nov 22 | r00 | SouthportSouthport | 1 - 3 | CurzonCurzon |
| 19 Nov 22 | r00 | Warrington TownWarrington To | 0 - 1 | GuiseleyGuiseley |
| 19 Nov 22 | r00 | MarineMarine | 1 - 0 | Scarborough AthleticScarborough A |
| 19 Nov 22 | r00 | Blyth SpartansBlyth Spartan | 4 - 2 | Buxton F.C.Buxton F.C. |
| 19 Nov 22 | r00 | Darlington F.C.Darlington F. | 4 - 1 | Alfreton TownAlfreton Town |
| 19 Nov 22 | r00 | AFC FyldeAFC Fylde | 2 - 1 | ColneColne |
| 19 Nov 22 | r00 | MatlockMatlock | 1 - 2 | Marske UnitedMarske United |
| 19 Nov 22 | r00 | Kings LangleyKings Langley | 1 - 2 | AFC Telford UnitedAFC Telford U |
| 19 Nov 22 | r00 | TamworthTamworth | 1 - 1 | Boston United FCBoston United |
| 19 Nov 22 | r00 | BanburyBanbury | 6 - 0 | Stowmarket TownStowmarket To |
| 19 Nov 22 | r00 | Heybridge SwiftsHeybridge Swi | 3 - 0 | HerefordHereford |
| 19 Nov 22 | r00 | LeamingtonLeamington | 1 - 0 | Bishops StortfordBishops Stort |
| 19 Nov 22 | r00 | Coalville TownCoalville Tow | 2 - 0 | Hednesford TownHednesford To |
| 19 Nov 22 | r00 | ChelmsfordChelmsford | 2 - 1 | RedditchRedditch |
| 19 Nov 22 | r00 | Braintree TownBraintree Tow | 1 - 0 | Brackley TownBrackley Town |
| 19 Nov 22 | r00 | LeistonLeiston | 4 - 3 | AlvechurchAlvechurch |
| 19 Nov 22 | r00 | Kings Lynn TownKings Lynn To | 1 - 1 | Hemel Hempstead Town FCHemel Hempste |
| 19 Nov 22 | r00 | Kettering TownKettering Tow | - | Gloucester CityGloucester Ci |
| 19 Nov 22 | r00 | St Albans CitySt Albans Cit | 1 - 2 | Kidderminster HarriersKidderminster |
| 19 Nov 22 | r00 | Peterborough SportsPeterborough | 3 - 2 | CheshuntCheshunt |
| 19 Nov 22 | r00 | Taunton TownTaunton Town | 2 - 1 | Dulwich HamletDulwich Hamle |
| 19 Nov 22 | r00 | Hampton and RichmondHampton and | 0 - 1 | FarnboroughFarnborough |
| 19 Nov 22 | r00 | TavistockTavistock | 1 - 2 | Hungerford TownHungerford To |
| 19 Nov 22 | r00 | Beaconsfield TownBeaconsfield | 1 - 1 | Folkestone InvictaFolkestone In |
| 19 Nov 22 | r00 | UxbridgeUxbridge | 1 - 1 | DoverDover |
| 19 Nov 22 | r00 | Hastings UnitedHastings Unit | 1 - 2 | Chippenham Town FCChippenham To |
| 19 Nov 22 | r00 | WorthingWorthing | 4 - 2 | WeymouthWeymouth |
| 19 Nov 22 | r00 | Oxford CityOxford City | 1 - 1 | Plymouth ParkwayPlymouth Park |
| 19 Nov 22 | r00 | Welling UnitedWelling Unite | 3 - 2 | Havant and WaterloovilleHavant and W |
| 19 Nov 22 | r00 | Concord RangersConcord Range | 2 - 4 | Harrow BoroughHarrow Boroug |
| 19 Nov 22 | r00 | LewesLewes | 2 - 2 | Bognor Regis TownBognor Regis |
| 19 Nov 22 | r00 | Hanwell TownHanwell Town | 0 - 3 | Slough TownSlough Town |
| 19 Nov 22 | r00 | Swindon SupermarineSwindon Super | 4 - 0 | DartfordDartford |
| 19 Nov 22 | r00 | BillericayBillericay | 1 - 3 | Bath CityBath City |
| 19 Nov 22 | r00 | Tonbridge AngelsTonbridge Ang | 2 - 4 | Bracknell TownBracknell Tow |
| 17 Dec 22 | r00 | TamworthTamworth | 2 - 1 | LeamingtonLeamington |
| 17 Dec 22 | r00 | YeovilYeovil | 0 - 0 | Dorking WanderersDorking Wande |
| 17 Dec 22 | r00 | Heybridge SwiftsHeybridge Swi | 1 - 1 | Hungerford TownHungerford To |
| 17 Dec 22 | r00 | Folkestone InvictaFolkestone In | 1 - 1 | LeistonLeiston |
| 17 Dec 22 | r00 | Eastbourne BoroughEastbourne Bo | 1 - 2 | Bracknell TownBracknell Tow |
| 20 Dec 22 | r00 | Spennymoor Town FCSpennymoor To | 1 - 3 | Darlington F.C.Darlington F. |
| 20 Dec 22 | r00 | YorkYork | 5 - 0 | Blyth SpartansBlyth Spartan |
| 20 Dec 22 | r00 | AltrinchamAltrincham | 1 - 0 | CurzonCurzon |
| 20 Dec 22 | r00 | Marske UnitedMarske United | 2 - 1 | MarineMarine |
| 20 Dec 22 | r00 | ChesterfieldChesterfield | 1 - 3 | Coalville TownCoalville Tow |
| 20 Dec 22 | r00 | AFC FyldeAFC Fylde | 1 - 1 | Kidderminster HarriersKidderminster |
| 20 Dec 22 | r00 | Gateshead FCGateshead FC | 5 - 1 | Kettering TownKettering Tow |
| 20 Dec 22 | r00 | Farsley CelticFarsley Celti | 2 - 2 | Chester CityChester City |
| 20 Dec 22 | r00 | Solihull MoorsSolihull Moor | 2 - 0 | AFC Telford UnitedAFC Telford U |
| 20 Dec 22 | r00 | Notts CountyNotts County | 2 - 1 | ChorleyChorley |
| 20 Dec 22 | r00 | OldhamOldham | 0 - 0 | Peterborough SportsPeterborough |
| 20 Dec 22 | r00 | GuiseleyGuiseley | 0 - 1 | HalifaxHalifax |
| 20 Dec 22 | r00 | SouthendSouthend | 2 - 0 | Boreham WoodBoreham Wood |
| 20 Dec 22 | r00 | WokingWoking | 0 - 2 | EastleighEastleigh |
| 20 Dec 22 | r00 | MaidstoneMaidstone | 2 - 0 | WorthingWorthing |
| 20 Dec 22 | r00 | FarnboroughFarnborough | 2 - 2 | Braintree TownBraintree Tow |
| 20 Dec 22 | r00 | BanburyBanbury | 4 - 3 | Bognor Regis TownBognor Regis |
| 20 Dec 22 | r00 | Slough TownSlough Town | 0 - 1 | Taunton TownTaunton Town |
| 20 Dec 22 | r00 | TorquayTorquay | 3 - 2 | Chippenham Town FCChippenham To |
| 20 Dec 22 | r00 | AldershotAldershot | 3 - 0 | Welling UnitedWelling Unite |
| 20 Dec 22 | r00 | Hemel Hempstead Town FCHemel Hempste | 2 - 3 | Maidenhead UnitedMaidenhead Un |
| 20 Dec 22 | r00 | BarnetBarnet | 1 - 0 | Plymouth ParkwayPlymouth Park |
| 20 Dec 22 | r00 | Bath CityBath City | 2 - 2 | BromleyBromley |
| 20 Dec 22 | r00 | Dagenham and RedbridgeDagenham and | 5 - 1 | UxbridgeUxbridge |
| 21 Dec 22 | r00 | WrexhamWrexham | 3 - 1 | ScunthorpeScunthorpe |
| 21 Dec 22 | r00 | Swindon SupermarineSwindon Super | 1 - 2 | Harrow BoroughHarrow Boroug |
| 04 Jan 23 | r00 | ChelmsfordChelmsford | 2 - 1 | WealdstoneWealdstone |
| |
| 13 Jan 23 | r04 | AltrinchamAltrincham | 2 - 2 | WrexhamWrexham |
| 14 Jan 23 | r04 | Hungerford TownHungerford To | 2 - 1 | TamworthTamworth |
| 14 Jan 23 | r04 | Gateshead FCGateshead FC | 3 - 1 | OldhamOldham |
| 14 Jan 23 | r04 | Dorking WanderersDorking Wande | 3 - 2 | Kidderminster HarriersKidderminster |
| 14 Jan 23 | r04 | Bracknell TownBracknell Tow | 1 - 0 | Bath CityBath City |
| 14 Jan 23 | r04 | Solihull MoorsSolihull Moor | 0 - 2 | BarnetBarnet |
| 14 Jan 23 | r04 | SouthendSouthend | 2 - 1 | Darlington F.C.Darlington F. |
| 14 Jan 23 | r04 | AldershotAldershot | 4 - 1 | LeistonLeiston |
| 14 Jan 23 | r04 | EastleighEastleigh | 1 - 0 | Braintree TownBraintree Tow |
| 14 Jan 23 | r04 | Farsley CelticFarsley Celti | 7 - 1 | Marske UnitedMarske United |
| 14 Jan 23 | r04 | YorkYork | 1 - 1 | ChelmsfordChelmsford |
| 14 Jan 23 | r04 | Dagenham and RedbridgeDagenham and | 0 - 2 | Maidenhead UnitedMaidenhead Un |
| 15 Jan 23 | r04 | Notts CountyNotts County | 2 - 2 | MaidstoneMaidstone |
| 17 Jan 23 | r04 | TorquayTorquay | 4 - 2 | Taunton TownTaunton Town |
| 31 Jan 23 | r04 | Harrow BoroughHarrow Boroug | 2 - 2 | HalifaxHalifax |
| 31 Jan 23 | r04 | BanburyBanbury | 3 - 2 | Coalville TownCoalville Tow |
| |
| 11 Feb 23 | r05 | Hungerford TownHungerford To | 2 - 3 | Farsley CelticFarsley Celti |
| 11 Feb 23 | r05 | SouthendSouthend | 0 - 2 | YorkYork |
| 11 Feb 23 | r05 | EastleighEastleigh | 0 - 4 | MaidstoneMaidstone |
| 11 Feb 23 | r05 | AldershotAldershot | 1 - 0 | Dorking WanderersDorking Wande |
| 11 Feb 23 | r05 | Bracknell TownBracknell Tow | 1 - 3 | AltrinchamAltrincham |
| 11 Feb 23 | r05 | TorquayTorquay | 1 - 2 | BarnetBarnet |
| 11 Feb 23 | r05 | Maidenhead UnitedMaidenhead Un | 0 - 0 | HalifaxHalifax |
| 11 Feb 23 | r05 | BanburyBanbury | 0 - 1 | Gateshead FCGateshead FC |
| |
| 11 Mar 23 | r00 | Gateshead FCGateshead FC | 0 - 0 | Farsley CelticFarsley Celti |
| 11 Mar 23 | r00 | MaidstoneMaidstone | 2 - 2 | BarnetBarnet |
| 11 Mar 23 | r00 | YorkYork | 1 - 2 | AltrinchamAltrincham |
| 11 Mar 23 | r00 | AldershotAldershot | 0 - 2 | HalifaxHalifax |
| 01 Apr 23 | r00 | Gateshead FCGateshead FC | 3 - 3 | BarnetBarnet |
| 01 Apr 23 | r00 | AltrinchamAltrincham | 1 - 1 | HalifaxHalifax |
| 21 May 23 | r00 | HalifaxHalifax | 1 - 0 | Gateshead FCGateshead FC |
| 21 May 23 | r00 | Gateshead FCGateshead FC | 1 - 0 | HalifaxHalifax |
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