| 12 Sep 22 | Qualifier  | MersthamMerstham | 3 - 0 | Hythe TownHythe Town |
| 12 Sep 22 | Qualifier | Brighouse TownBrighouse Tow | 0 - 2 | Worksop TownWorksop Town |
| 12 Sep 22 | Qualifier | Grantham TownGrantham Town | 1 - 2 | Sutton Coldfield TownSutton Coldfi |
| 13 Sep 22 | Qualifier | Willand RoversWilland Rover | 0 - 3 | BidefordBideford |
| 13 Sep 22 | Qualifier | Ashford UnitedAshford Unite | 3 - 3 | AFC SudburyAFC Sudbury |
| 13 Sep 22 | Qualifier | Maldon & TiptreeMaldon & Tipt | 0 - 3 | WareWare |
| 13 Sep 22 | Qualifier | FC RomaniaFC Romania | 1 - 1 | Westfield SurreyWestfield Sur |
| 13 Sep 22 | Qualifier | Three BridgesThree Bridges | 3 - 2 | Hullbridge SportsHullbridge Sp |
| 13 Sep 22 | Qualifier | Walton & HershamWalton & Hers | 3 - 1 | LeatherheadLeatherhead |
| 13 Sep 22 | Qualifier | Great Wakering RoversGreat Wakerin | 1 - 2 | Harlow TownHarlow Town |
| 13 Sep 22 | Qualifier | VCD AthleticVCD Athletic | 0 - 3 | GraysGrays |
| 13 Sep 22 | Qualifier | Tooting & Mitcham UnitedTooting & Mit | 0 - 0 | CorinthianCorinthian |
| 13 Sep 22 | Qualifier | Witham TownWitham Town | 1 - 0 | Brentwood TownBrentwood Tow |
| 13 Sep 22 | Qualifier | Felixstowe & Walton UniteFelixstowe & | 2 - 2 | Burgess Hill TownBurgess Hill |
| 13 Sep 22 | Qualifier | TilburyTilbury | 1 - 2 | WhitehawkWhitehawk |
| 13 Sep 22 | Qualifier | LancingLancing | 1 - 4 | Cray Valley PMCray Valley P |
| 13 Sep 22 | Qualifier | WroxhamWroxham | 4 - 1 | East Grinstead TownEast Grinstea |
| 13 Sep 22 | Qualifier | Welwyn Garden CityWelwyn Garden | 1 - 2 | New SalamisNew Salamis |
| 13 Sep 22 | Qualifier | Bedfont SportsBedfont Sport | 2 - 2 | Hashtag UnitedHashtag Unite |
| 13 Sep 22 | Qualifier | Barton RoversBarton Rovers | 0 - 1 | Chatham TownChatham Town |
| 13 Sep 22 | Qualifier | AFC TottonAFC Totton | 1 - 1 | SlimbridgeSlimbridge |
| 13 Sep 22 | Qualifier | Highworth TownHighworth Tow | 1 - 2 | Paulton RoversPaulton Rover |
| 13 Sep 22 | Qualifier | Wimborne TownWimborne Town | 2 - 3 | Bristol Manor FarmBristol Manor |
| 13 Sep 22 | Qualifier | Thame UnitedThame United | 0 - 2 | Westbury UnitedWestbury Unit |
| 13 Sep 22 | Qualifier | KidlingtonKidlington | 0 - 0 | TavistockTavistock |
| 13 Sep 22 | Qualifier | MarlowMarlow | 0 - 4 | BinfieldBinfield |
| 13 Sep 22 | Qualifier | BashleyBashley | 4 - 1 | Frome TownFrome Town |
| 13 Sep 22 | Qualifier | BerkhamstedBerkhamsted | 5 - 0 | ExmouthExmouth |
| 13 Sep 22 | Qualifier | BasingstokeBasingstoke | 3 - 5 | CirencesterCirencester |
| 13 Sep 22 | Qualifier | Bishops CleeveBishops Cleev | 2 - 3 | AFC DunstableAFC Dunstable |
| 13 Sep 22 | Qualifier | WalthamstowWalthamstow | 1 - 3 | Ashford Town MiddlesexAshford Town |
| 13 Sep 22 | Qualifier | SholingSholing | 0 - 1 | Lymington TownLymington Tow |
| 13 Sep 22 | Qualifier | Didcot TownDidcot Town | 1 - 1 | Hamworthy UnitedHamworthy Uni |
| 13 Sep 22 | Qualifier | Cinderford TownCinderford To | 0 - 1 | Thatcham TownThatcham Town |
| 13 Sep 22 | Qualifier | Littlehampton TownLittlehampton | 1 - 0 | RamsgateRamsgate |
| 13 Sep 22 | Qualifier | Haywards Heath TownHaywards Heat | 0 - 0 | SouthallSouthall |
| 13 Sep 22 | Qualifier | Chichester CityChichester Ci | 0 - 1 | Faversham TownFaversham Tow |
| 13 Sep 22 | Qualifier | Stamford AFCStamford AFC | 1 - 2 | ChasetownChasetown |
| 13 Sep 22 | Qualifier | DaventryDaventry | 2 - 3 | Lincoln UnitedLincoln Unite |
| 13 Sep 22 | Qualifier | Newcastle TownNewcastle Tow | 0 - 2 | Spalding UnitedSpalding Unit |
| 13 Sep 22 | Qualifier | Bedworth UnitedBedworth Unit | 0 - 1 | Harborough TownHarborough To |
| 13 Sep 22 | Qualifier | Bridlington TownBridlington T | 1 - 2 | ColneColne |
| 13 Sep 22 | Qualifier | North ShieldsNorth Shields | 3 - 1 | Pontefract CollieriesPontefract Co |
| 13 Sep 22 | Qualifier | WorkingtonWorkington | 1 - 2 | Runcorn LinnetsRuncorn Linne |
| 13 Sep 22 | Qualifier | MossleyMossley | 1 - 2 | Dunston UTSDunston UTS |
| 13 Sep 22 | Qualifier | Prescot CablesPrescot Cable | 1 - 1 | Cleethorpes TownCleethorpes T |
| 13 Sep 22 | Qualifier | ClitheroeClitheroe | 2 - 1 | 1874 Northwich1874 Northwic |
| 13 Sep 22 | Qualifier | BootleBootle | - | Grimsby BoroughGrimsby Borou |
| 13 Sep 22 | Qualifier | WittonWitton | 3 - 4 | Stockton TownStockton Town |
| 13 Sep 22 | Qualifier | Stocksbridge Park SteelsStocksbridge | 2 - 2 | Ossett UnitedOssett United |
| 13 Sep 22 | Qualifier | MacclesfieldMacclesfield | 7 - 0 | Tadcaster AlbionTadcaster Alb |
| 13 Sep 22 | Qualifier | Shildon AFCShildon AFC | 1 - 2 | ConsettConsett |
| 13 Sep 22 | Qualifier | Sheppey UnitedSheppey Unite | 3 - 3 | Hertford TownHertford Town |
| 13 Sep 22 | Qualifier | Bury TownBury Town | 1 - 2 | Stowmarket TownStowmarket To |
| 13 Sep 22 | Qualifier | East Thurrock UnitedEast Thurrock | 3 - 1 | Sutton Common RoversSutton Common |
| 13 Sep 22 | Qualifier | Beckenham TownBeckenham Tow | 1 - 1 | Heybridge SwiftsHeybridge Swi |
| 13 Sep 22 | Qualifier | Chertsey TownChertsey Town | 1 - 3 | UxbridgeUxbridge |
| 13 Sep 22 | Qualifier | ChipsteadChipstead | 4 - 2 | Waltham AbbeyWaltham Abbey |
| 13 Sep 22 | Qualifier | NorthwoodNorthwood | 0 - 3 | Hanworth VillaHanworth Vill |
| 13 Sep 22 | Qualifier | Basildon UnitedBasildon Unit | 0 - 2 | Lowestoft TownLowestoft Tow |
| 13 Sep 22 | Qualifier | AFC Kempston RoversAFC Kempston | 0 - 1 | Dereham TownDereham Town |
| 13 Sep 22 | Qualifier | Ramsbottom UnitedRamsbottom Un | 2 - 1 | Glossop North EndGlossop North |
| 13 Sep 22 | Qualifier | Kidsgrove AthleticKidsgrove Ath | 3 - 4 | Hinckley Leicester RoadHinckley Leic |
| 13 Sep 22 | Qualifier | Halesowen TownHalesowen Tow | 5 - 2 | BiggleswadeBiggleswade |
| 13 Sep 22 | Qualifier | Boldmere St MichaelsBoldmere St M | 4 - 3 | Sporting KhalsaSporting Khal |
| 13 Sep 22 | Qualifier | Loughborough DynamoLoughborough | 3 - 1 | Corby TownCorby Town |
| 13 Sep 22 | Qualifier | Coleshill TownColeshill Tow | 3 - 2 | St Neots TownSt Neots Town |
| 13 Sep 22 | Qualifier | Shepshed DynamoShepshed Dyna | 1 - 0 | Biggleswade TownBiggleswade T |
| 13 Sep 22 | Qualifier | Long Eaton UnitedLong Eaton Un | 2 - 0 | Gresley RoversGresley Rover |
| 13 Sep 22 | Qualifier | Carlton TownCarlton Town | 2 - 0 | Hanley TownHanley Town |
| 13 Sep 22 | Qualifier | TraffordTrafford | 1 - 2 | Hebburn TownHebburn Town |
| 14 Sep 22 | Qualifier | HadleyHadley | 1 - 2 | Sevenoaks TownSevenoaks Tow |
| 14 Sep 22 | Qualifier | South ParkSouth Park | 1 - 2 | SittingbourneSittingbourne |
| 14 Sep 22 | Qualifier | Larkhall AthleticLarkhall Athl | 0 - 2 | Melksham TownMelksham Town |
| 14 Sep 22 | Qualifier | AylesburyAylesbury | 0 - 3 | Evesham UnitedEvesham Unite |
| 14 Sep 22 | Qualifier | Cambridge CityCambridge Cit | 4 - 1 | YaxleyYaxley |
| 14 Sep 22 | Qualifier | WidnesWidnes | 1 - 1 | Skelmersdale UnitedSkelmersdale |
| 14 Sep 22 | Qualifier | City of LiverpoolCity of Liver | 0 - 0 | Sheffield FCSheffield FC |
| 20 Sep 22 | Qualifier | GorlestonGorleston | 1 - 1 | Coggeshall TownCoggeshall To |
| |
| 23 Sep 22 | r00 | Hebburn TownHebburn Town | 3 - 1 | Stockton TownStockton Town |
| 24 Sep 22 | r00 | Prescot CablesPrescot Cable | 1 - 3 | Worksop TownWorksop Town |
| 24 Sep 22 | r00 | BootleBootle | 0 - 3 | Dunston UTSDunston UTS |
| 24 Sep 22 | r00 | ColneColne | 1 - 0 | ConsettConsett |
| 24 Sep 22 | r00 | Ramsbottom UnitedRamsbottom Un | 1 - 4 | ClitheroeClitheroe |
| 24 Sep 22 | r00 | North ShieldsNorth Shields | 1 - 2 | Runcorn LinnetsRuncorn Linne |
| 24 Sep 22 | r00 | Coleshill TownColeshill Tow | 4 - 4 | Leek TownLeek Town |
| 24 Sep 22 | r00 | Long Eaton UnitedLong Eaton Un | 0 - 0 | Loughborough DynamoLoughborough |
| 24 Sep 22 | r00 | Dereham TownDereham Town | 0 - 1 | Spalding UnitedSpalding Unit |
| 24 Sep 22 | r00 | Harborough TownHarborough To | 2 - 1 | Shepshed DynamoShepshed Dyna |
| 24 Sep 22 | r00 | Lincoln UnitedLincoln Unite | 1 - 1 | Carlton TownCarlton Town |
| 24 Sep 22 | r00 | Sutton Coldfield TownSutton Coldfi | 2 - 0 | BiggleswadeBiggleswade |
| 24 Sep 22 | r00 | Boldmere St MichaelsBoldmere St M | 1 - 1 | ChasetownChasetown |
| 24 Sep 22 | r00 | Cray Valley PMCray Valley P | 5 - 3 | East Thurrock UnitedEast Thurrock |
| 24 Sep 22 | r00 | Chatham TownChatham Town | 0 - 1 | Stowmarket TownStowmarket To |
| 24 Sep 22 | r00 | Hanworth VillaHanworth Vill | 3 - 2 | Littlehampton TownLittlehampton |
| 24 Sep 22 | r00 | UxbridgeUxbridge | 2 - 0 | Bedfont SportsBedfont Sport |
| 24 Sep 22 | r00 | WroxhamWroxham | 5 - 1 | Lowestoft TownLowestoft Tow |
| 24 Sep 22 | r00 | CorinthianCorinthian | 2 - 4 | WhitehawkWhitehawk |
| 24 Sep 22 | r00 | Ashford UnitedAshford Unite | 2 - 0 | Three BridgesThree Bridges |
| 24 Sep 22 | r00 | Heybridge SwiftsHeybridge Swi | 2 - 1 | ChipsteadChipstead |
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