| 12 Sep 25 | r02        | Folkestone InvictaFolkestone In | 2 - 0 | Maidstone UnitedMaidstone Uni |
| 13 Sep 25 | r02        | Pickering TownPickering Tow | 0 - 2 | Runcorn LinnetsRuncorn Linne |
| 13 Sep 25 | r02        | Chelmsford CityChelmsford Ci | 6 - 0 | Hertford TownHertford Town |
| 13 Sep 25 | r02        | Morpeth TownMorpeth Town | 2 - 1 | Witton AlbionWitton Albion |
| 13 Sep 25 | r02        | Ashton UnitedAshton United | 2 - 0 | Scarborough AthleticScarborough A |
| 13 Sep 25 | r02        | Bracknell TownBracknell Tow | 2 - 0 | Tadley CallevaTadley Callev |
| 13 Sep 25 | r02        | Stalybridge CelticStalybridge C | 1 - 2 | ChesterChester |
| 13 Sep 25 | r02        | Hitchin TownHitchin Town | 1 - 2 | St Albans CitySt Albans Cit |
| 13 Sep 25 | r02        | Chesham UnitedChesham Unite | 1 - 4 | King's Lynn TownKing's Lynn T |
| 13 Sep 25 | r02        | Hemel Hempstead TownHemel Hempste | 4 - 1 | Bishops StortfordBishops Stort |
| 13 Sep 25 | r02        | Hyde UnitedHyde United | 2 - 0 | Whitby TownWhitby Town |
| 13 Sep 25 | r02        | WhitehawkWhitehawk | 0 - 2 | Walton and HershamWalton and He |
| 13 Sep 25 | r02        | Needham MarketNeedham Marke | 4 - 2 | Eynesbury RoversEynesbury Rov |
| 13 Sep 25 | r02        | Gainsborough TrinityGainsborough | 2 - 1 | Rushall OlympicRushall Olymp |
| 13 Sep 25 | r02        | Westfield SurreyWestfield Sur | 2 - 2 | HorshamHorsham |
| 13 Sep 25 | r02        | Matlock TownMatlock Town | 3 - 0 | Carlton TownCarlton Town |
| 13 Sep 25 | r02        | West Auckland TownWest Auckland | 0 - 1 | Spennymoor TownSpennymoor To |
| 13 Sep 25 | r02        | Maldon and TiptreeMaldon and Ti | 2 - 0 | Stanway RoversStanway Rover |
| 13 Sep 25 | r02        | Shepshed DynamoShepshed Dyna | 0 - 2 | StamfordStamford |
| 13 Sep 25 | r02        | Curzon AshtonCurzon Ashton | 4 - 1 | Hebburn TownHebburn Town |
| 13 Sep 25 | r02        | SalisburySalisbury | 4 - 1 | Laverstock and FordLaverstock an |
| 13 Sep 25 | r02        | AlvechurchAlvechurch | 3 - 0 | LeamingtonLeamington |
| 13 Sep 25 | r02        | EnfieldEnfield | 0 - 3 | Enfield TownEnfield Town |
| 13 Sep 25 | r02        | Bury TownBury Town | 1 - 1 | Woodford TownWoodford Town |
| 13 Sep 25 | r02        | Hanwell TownHanwell Town | 0 - 1 | Bedfont SportsBedfont Sport |
| 13 Sep 25 | r02        | Nantwich TownNantwich Town | 3 - 1 | TraffordTrafford |
| 13 Sep 25 | r02        | Royston TownRoyston Town | 1 - 0 | Brentwood TownBrentwood Tow |
| 13 Sep 25 | r02        | Newcastle Blue StarNewcastle Blu | 0 - 2 | MarineMarine |
| 13 Sep 25 | r02        | Gosport BoroughGosport Borou | 0 - 3 | Poole TownPoole Town |
| 13 Sep 25 | r02        | LeistonLeiston | 4 - 1 | Hackney WickHackney Wick |
| 13 Sep 25 | r02        | Whitstable TownWhitstable To | 1 - 1 | Chichester CityChichester Ci |
| 13 Sep 25 | r02        | Hungerford TownHungerford To | 3 - 0 | Swindon SupermarineSwindon Super |
| 13 Sep 25 | r02        | Steyning TownSteyning Town | 2 - 2 | Tonbridge AngelsTonbridge Ang |
| 13 Sep 25 | r02        | Welling UnitedWelling Unite | 1 - 1 | Slough TownSlough Town |
| 13 Sep 25 | r02        | Sutton Coldfield TownSutton Coldfi | 3 - 1 | StourbridgeStourbridge |
| 13 Sep 25 | r02        | AFC FyldeAFC Fylde | 4 - 1 | Bamber BridgeBamber Bridge |
| 13 Sep 25 | r02        | Ashford UnitedAshford Unite | 0 - 3 | Chatham TownChatham Town |
| 13 Sep 25 | r02        | Jersey BullsJersey Bulls | 2 - 2 | WorthingWorthing |
| 13 Sep 25 | r02        | Congleton TownCongleton Tow | 0 - 1 | ChorleyChorley |
| 13 Sep 25 | r02        | Gloucester CityGloucester Ci | 1 - 2 | Chippenham TownChippenham To |
| 13 Sep 25 | r02        | South ShieldsSouth Shields | 2 - 1 | GuiseleyGuiseley |
| 13 Sep 25 | r02        | Dorking WanderersDorking Wande | 7 - 2 | Wingate and FinchleyWingate and F |
| 13 Sep 25 | r02        | Racing Club WarwickRacing Club W | 3 - 3 | Evesham UnitedEvesham Unite |
| 13 Sep 25 | r02        | FarnboroughFarnborough | 4 - 1 | Dover AthleticDover Athleti |
| 13 Sep 25 | r02        | Spalding UnitedSpalding Unit | 3 - 0 | Alfreton TownAlfreton Town |
| 13 Sep 25 | r02        | Burgess Hill TownBurgess Hill | 1 - 3 | Farnham TownFarnham Town |
| 13 Sep 25 | r02        | Deal TownDeal Town | 2 - 1 | Egham TownEgham Town |
| 13 Sep 25 | r02        | Coleshill TownColeshill Tow | 0 - 7 | Hednesford TownHednesford To |
| 13 Sep 25 | r02        | Harborough TownHarborough To | 3 - 2 | Worksop TownWorksop Town |
| 13 Sep 25 | r02        | Grimsby BoroughGrimsby Borou | 1 - 1 | Halesowen TownHalesowen Tow |
| 13 Sep 25 | r02        | BootleBootle | 1 - 3 | DarlingtonDarlington |
| 13 Sep 25 | r02        | BrixhamBrixham | 1 - 3 | Dorchester TownDorchester To |
| 13 Sep 25 | r02        | Dunston UTSDunston UTS | 1 - 0 | Stocksbridge Park SteelsStocksbridge |
| 13 Sep 25 | r02        | AFC TottonAFC Totton | 2 - 0 | Torquay UnitedTorquay Unite |
| 13 Sep 25 | r02        | Bedford TownBedford Town | 1 - 1 | Dagenham and RedbridgeDagenham and |
| 13 Sep 25 | r02        | Peterborough SportsPeterborough | 2 - 1 | HornchurchHornchurch |
| 13 Sep 25 | r02        | BuxtonBuxton | 3 - 0 | Redditch UnitedRedditch Unit |
| 13 Sep 25 | r02        | ChasetownChasetown | 0 - 0 | Banbury UnitedBanbury Unite |
| 13 Sep 25 | r02        | Soul Tower HamletsSoul Tower Ha | 0 - 2 | Flackwell HeathFlackwell Hea |
| 13 Sep 25 | r02        | Fareham TownFareham Town | 0 - 3 | SholingSholing |
| 13 Sep 25 | r02        | Wimborne TownWimborne Town | 2 - 1 | Bath CityBath City |
| 13 Sep 25 | r02        | Maidenhead UnitedMaidenhead Un | 0 - 1 | Faversham TownFaversham Tow |
| 13 Sep 25 | r02        | AFC Telford UnitedAFC Telford U | 3 - 1 | Kidderminster HarriersKidderminster |
| 13 Sep 25 | r02        | Billericay TownBillericay To | 3 - 0 | BerkhamstedBerkhamsted |
| 13 Sep 25 | r02        | Chertsey TownChertsey Town | 2 - 3 | Cray Valley Paper MillsCray Valley P |
| 13 Sep 25 | r02        | Ebbsfleet UnitedEbbsfleet Uni | 5 - 0 | Ashford Town MiddlesexAshford Town |
| 13 Sep 25 | r02        | Eastbourne BoroughEastbourne Bo | 4 - 0 | Epsom and EwellEpsom and Ewe |
| 13 Sep 25 | r02        | FC United of ManchesterFC United of | 0 - 1 | ChaddertonChadderton |
| 13 Sep 25 | r02        | QuornQuorn | 2 - 1 | Kettering TownKettering Tow |
| 13 Sep 25 | r02        | ShaftesburyShaftesbury | 1 - 1 | Frome TownFrome Town |
| 13 Sep 25 | r02        | Taunton TownTaunton Town | 1 - 1 | Weston-super-MareWeston-super- |
| 13 Sep 25 | r02        | Westbury UnitedWestbury Unit | 3 - 2 | Oxford CityOxford City |
| 13 Sep 25 | r02        | MacclesfieldMacclesfield | 3 - 0 | Atherton Laburnum RoversAtherton Labu |
| 13 Sep 25 | r02        | Merthyr TownMerthyr Town | 4 - 0 | Torpoint AthleticTorpoint Athl |
| 13 Sep 25 | r02        | AFC SudburyAFC Sudbury | 1 - 2 | AveleyAveley |
| 13 Sep 25 | r02        | Mulbarton WanderersMulbarton Wan | 0 - 0 | Witham TownWitham Town |
| 13 Sep 25 | r02        | RadcliffeRadcliffe | 1 - 1 | SouthportSouthport |
| 13 Sep 25 | r02        | Waltham AbbeyWaltham Abbey | 2 - 0 | GorlestonGorleston |
| 16 Sep 25 | r02        | SouthportSouthport | 3 - 2 | RadcliffeRadcliffe |
| 16 Sep 25 | r02        | Dagenham and RedbridgeDagenham and | 1 - 0 | Bedford TownBedford Town |
| 16 Sep 25 | r02        | Weston-super-MareWeston-super- | 3 - 0 | Taunton TownTaunton Town |
| 16 Sep 25 | r02        | WorthingWorthing | 5 - 0 | Jersey BullsJersey Bulls |
| 16 Sep 25 | r02        | Slough TownSlough Town | 1 - 0 | Welling UnitedWelling Unite |
| 16 Sep 25 | r02        | HorshamHorsham | 6 - 0 | Westfield SurreyWestfield Sur |
| 16 Sep 25 | r02        | Tonbridge AngelsTonbridge Ang | 6 - 1 | Steyning TownSteyning Town |
| 16 Sep 25 | r02        | Banbury UnitedBanbury Unite | 1 - 1 | ChasetownChasetown |
| 16 Sep 25 | r02        | Halesowen TownHalesowen Tow | 6 - 1 | Grimsby BoroughGrimsby Borou |
| 16 Sep 25 | r02        | Chichester CityChichester Ci | 0 - 1 | Whitstable TownWhitstable To |
| 16 Sep 25 | r02        | Evesham UnitedEvesham Unite | 1 - 0 | Racing Club WarwickRacing Club W |
| 16 Sep 25 | r02        | Witham TownWitham Town | 2 - 0 | Mulbarton WanderersMulbarton Wan |
| 17 Sep 25 | r02        | Frome TownFrome Town | 1 - 1 | ShaftesburyShaftesbury |
| 17 Sep 25 | r02        | Woodford TownWoodford Town | 1 - 0 | Bury TownBury Town |
| 23 Sep 25 | r02        | Sporting KhalsaSporting Khal | 2 - 3 | HerefordHereford |