| 31 Oct 25 | r01        | Luton TownLuton Town | 4 - 3 | Forest Green RoversForest Green |
| 01 Nov 25 | r01        | Chelmsford CityChelmsford Ci | 4 - 1 | Braintree TownBraintree Tow |
| 01 Nov 25 | r01        | AFC WimbledonAFC Wimbledon | 0 - 2 | GatesheadGateshead |
| 01 Nov 25 | r01        | BarnsleyBarnsley | 3 - 2 | York CityYork City |
| 01 Nov 25 | r01        | BlackpoolBlackpool | 1 - 0 | Scunthorpe UnitedScunthorpe Un |
| 01 Nov 25 | r01        | Bolton WanderersBolton Wander | 2 - 1 | Huddersfield TownHuddersfield |
| 01 Nov 25 | r01        | Boreham WoodBoreham Wood | 3 - 0 | Crawley TownCrawley Town |
| 01 Nov 25 | r01        | BromleyBromley | 1 - 2 | Bristol RoversBristol Rover |
| 01 Nov 25 | r01        | BuxtonBuxton | 2 - 1 | Chatham TownChatham Town |
| 01 Nov 25 | r01        | Cheltenham TownCheltenham To | 1 - 0 | Bradford CityBradford City |
| 01 Nov 25 | r01        | Colchester UnitedColchester Un | 2 - 3 | Milton Keynes DonsMilton Keynes |
| 01 Nov 25 | r01        | Crewe AlexandraCrewe Alexand | 1 - 2 | Doncaster RoversDoncaster Rov |
| 01 Nov 25 | r01        | Halifax TownHalifax Town | 0 - 2 | Exeter CityExeter City |
| 01 Nov 25 | r01        | Fleetwood TownFleetwood Tow | 2 - 1 | BarnetBarnet |
| 01 Nov 25 | r01        | Grimsby TownGrimsby Town | 3 - 1 | Ebbsfleet UnitedEbbsfleet Uni |
| 01 Nov 25 | r01        | Mansfield TownMansfield Tow | 3 - 2 | Harrogate TownHarrogate Tow |
| 01 Nov 25 | r01        | Newport CountyNewport Count | 1 - 1 | GillinghamGillingham |
| 01 Nov 25 | r01        | Oldham AthleticOldham Athlet | 3 - 1 | Northampton TownNorthampton T |
| 01 Nov 25 | r01        | Peterborough UnitedPeterborough | 1 - 0 | Cardiff CityCardiff City |
| 01 Nov 25 | r01        | ReadingReading | 2 - 3 | Carlisle UnitedCarlisle Unit |
| 01 Nov 25 | r01        | Rotherham UnitedRotherham Uni | 1 - 2 | Swindon TownSwindon Town |
| 01 Nov 25 | r01        | Salford CitySalford City | 1 - 1 | Lincoln CityLincoln City |
| 01 Nov 25 | r01        | Spennymoor TownSpennymoor To | 0 - 2 | BarrowBarrow |
| 01 Nov 25 | r01        | StevenageStevenage | 0 - 1 | ChesterfieldChesterfield |
| 01 Nov 25 | r01        | Tranmere RoversTranmere Rove | 1 - 3 | Stockport CountyStockport Cou |
| 01 Nov 25 | r01        | WealdstoneWealdstone | 1 - 0 | Southend UnitedSouthend Unit |
| 01 Nov 25 | r01        | Weston-super-MareWeston-super- | 2 - 1 | Aldershot TownAldershot Tow |
| 01 Nov 25 | r01        | Wigan AthleticWigan Athleti | 1 - 1 | Hemel Hempstead TownHemel Hempste |
| 01 Nov 25 | r01        | Wycombe WanderersWycombe Wande | 2 - 0 | Plymouth ArgylePlymouth Argy |
| 01 Nov 25 | r01        | Burton AlbionBurton Albion | 6 - 0 | St Albans CitySt Albans Cit |
| 01 Nov 25 | r01        | Cambridge UnitedCambridge Uni | 3 - 0 | ChesterChester |
| 01 Nov 25 | r01        | MacclesfieldMacclesfield | 6 - 3 | AFC TottonAFC Totton |
| 01 Nov 25 | r01        | Slough TownSlough Town | 2 - 1 | AltrinchamAltrincham |
| 01 Nov 25 | r01        | Sutton UnitedSutton United | 2 - 1 | AFC Telford UnitedAFC Telford U |
| 01 Nov 25 | r01        | Brackley TownBrackley Town | 2 - 2 | Notts CountyNotts County |
| 02 Nov 25 | r01        | South ShieldsSouth Shields | 1 - 3 | Shrewsbury TownShrewsbury To |
| 02 Nov 25 | r01        | EastleighEastleigh | 0 - 3 | WalsallWalsall |
| 02 Nov 25 | r01        | Port ValePort Vale | 5 - 1 | Maldon and TiptreeMaldon and Ti |
| 02 Nov 25 | r01        | Gainsborough TrinityGainsborough | 1 - 2 | Accrington StanleyAccrington St |
| 03 Nov 25 | r01        | TamworthTamworth | 0 - 1 | Leyton OrientLeyton Orient |