| 04 Aug 23 | Qualifier  | Skelmersdale UnitedSkelmersdale | 3 - 1 | Northwich VictoriaNorthwich Vic |
| 04 Aug 23 | Qualifier | Edgware TownEdgware Town | 1 - 1 | Waltham AbbeyWaltham Abbey |
| 05 Aug 23 | Qualifier | Kendal TownKendal Town | 1 - 4 | ConsettConsett |
| 05 Aug 23 | Qualifier | Ossett UnitedOssett United | 2 - 0 | 1874 Northwich1874 Northwic |
| 05 Aug 23 | Qualifier | Glossop North EndGlossop North | 1 - 2 | ColneColne |
| 05 Aug 23 | Qualifier | Cleethorpes TownCleethorpes T | 1 - 1 | Loughborough DynamoLoughborough |
| 05 Aug 23 | Qualifier | YaxleyYaxley | 0 - 7 | WroxhamWroxham |
| 05 Aug 23 | Qualifier | FC RomaniaFC Romania | 5 - 0 | TilburyTilbury |
| 05 Aug 23 | Qualifier | Ardley UnitedArdley United | 1 - 1 | Ascot UnitedAscot United |
| 05 Aug 23 | Qualifier | Thame UnitedThame United | 2 - 3 | Bristol Manor FarmBristol Manor |
| 05 Aug 23 | Qualifier | Sutton Common RoversSutton Common | 1 - 1 | Burgess Hill TownBurgess Hill |
| 05 Aug 23 | Qualifier | Haywards Heath TownHaywards Heat | 0 - 5 | Whitstable TownWhitstable To |
| 05 Aug 23 | Qualifier | CorinthianCorinthian | 3 - 4 | Bedfont SportsBedfont Sport |
| 05 Aug 23 | Qualifier | SouthallSouthall | 1 - 0 | Chichester CityChichester Ci |
| 05 Aug 23 | Qualifier | Paulton RoversPaulton Rover | 5 - 1 | Thatcham TownThatcham Town |
| 05 Aug 23 | Qualifier | Ashington AFCAshington AFC | 4 - 0 | North ShieldsNorth Shields |
| 05 Aug 23 | Qualifier | Rugby TownRugby Town | 5 - 5 | Malvern TownMalvern Town |
| 05 Aug 23 | Qualifier | Kidsgrove AthleticKidsgrove Ath | 0 - 0 | Lye TownLye Town |
| 05 Aug 23 | Qualifier | QuornQuorn | 1 - 1 | Carlton TownCarlton Town |
| 05 Aug 23 | Qualifier | Boston United FCBoston United | 0 - 0 | Anstey NomadsAnstey Nomads |
| 05 Aug 23 | Qualifier | RedbridgeRedbridge | 2 - 2 | New SalamisNew Salamis |
| 05 Aug 23 | Qualifier | Leighton TownLeighton Town | 3 - 0 | Basildon UnitedBasildon Unit |
| 05 Aug 23 | Qualifier | MersthamMerstham | 2 - 1 | Raynes Park ValeRaynes Park V |
| 06 Aug 23 | Qualifier | Enfield FCEnfield FC | 2 - 0 | Coggeshall TownCoggeshall To |
| 06 Aug 23 | Qualifier | Littlehampton TownLittlehampton | 0 - 6 | Erith and BelvedereErith and Bel |
| 08 Aug 23 | Qualifier | Burgess Hill TownBurgess Hill | 2 - 1 | Sutton Common RoversSutton Common |
| 08 Aug 23 | Qualifier | Ipswich WanderersIpswich Wande | 3 - 0 | HistonHiston |
| 08 Aug 23 | Qualifier | Waltham AbbeyWaltham Abbey | 2 - 1 | Edgware TownEdgware Town |
| 08 Aug 23 | Qualifier | Malvern TownMalvern Town | 1 - 2 | Rugby TownRugby Town |
| 08 Aug 23 | Qualifier | Lye TownLye Town | 1 - 2 | Kidsgrove AthleticKidsgrove Ath |
| 08 Aug 23 | Qualifier | Carlton TownCarlton Town | 2 - 0 | QuornQuorn |
| 08 Aug 23 | Qualifier | Loughborough DynamoLoughborough | 0 - 2 | Cleethorpes TownCleethorpes T |
| 08 Aug 23 | Qualifier | Anstey NomadsAnstey Nomads | 2 - 2 | Boston United FCBoston United |
| 08 Aug 23 | Qualifier | Ascot UnitedAscot United | 2 - 1 | Ardley UnitedArdley United |
| 09 Aug 23 | Qualifier | New SalamisNew Salamis | 3 - 1 | RedbridgeRedbridge |
| 18 Aug 23 | Qualifier | Skelmersdale UnitedSkelmersdale | 0 - 6 | Nantwich TownNantwich Town |
| 18 Aug 23 | Qualifier | Ipswich WanderersIpswich Wande | 0 - 0 | Dereham TownDereham Town |
| 19 Aug 23 | Qualifier | Runcorn LinnetsRuncorn Linne | 3 - 2 | StalybridgeStalybridge |
| 19 Aug 23 | Qualifier | Belper TownBelper Town | 2 - 5 | Leek TownLeek Town |
| 19 Aug 23 | Qualifier | Corby TownCorby Town | 0 - 4 | Lowestoft TownLowestoft Tow |
| 19 Aug 23 | Qualifier | Brentwood TownBrentwood Tow | 2 - 1 | Kings LangleyKings Langley |
| 19 Aug 23 | Qualifier | South ParkSouth Park | 1 - 1 | LeatherheadLeatherhead |
| 19 Aug 23 | Qualifier | Stockton TownStockton Town | 2 - 1 | Ashington AFCAshington AFC |
| 19 Aug 23 | Qualifier | LiversedgeLiversedge | 0 - 1 | North Ferriby FCNorth Ferriby |
| 19 Aug 23 | Qualifier | MossleyMossley | 1 - 0 | Ramsbottom UnitedRamsbottom Un |
| 19 Aug 23 | Qualifier | ColneColne | 1 - 3 | Brighouse TownBrighouse Tow |
| 19 Aug 23 | Qualifier | Boldmere St MichaelsBoldmere St M | 1 - 0 | ChasetownChasetown |
| 19 Aug 23 | Qualifier | AtherstoneAtherstone | 2 - 0 | Gresley RoversGresley Rover |
| 19 Aug 23 | Qualifier | Wisbech TownWisbech Town | 1 - 5 | Biggleswade TownBiggleswade T |
| 19 Aug 23 | Qualifier | AFC Kempston RoversAFC Kempston | 1 - 3 | BiggleswadeBiggleswade |
| 19 Aug 23 | Qualifier | Bury TownBury Town | 1 - 2 | WroxhamWroxham |
| 19 Aug 23 | Qualifier | East Thurrock UnitedEast Thurrock | 1 - 1 | Barton RoversBarton Rovers |
| 19 Aug 23 | Qualifier | WareWare | 2 - 1 | AFC DunstableAFC Dunstable |
| 19 Aug 23 | Qualifier | RomfordRomford | 1 - 3 | Welwyn Garden CityWelwyn Garden |
| 19 Aug 23 | Qualifier | Heybridge SwiftsHeybridge Swi | 2 - 0 | BarkingBarking |
| 19 Aug 23 | Qualifier | Faversham TownFaversham Tow | 2 - 1 | MersthamMerstham |
| 19 Aug 23 | Qualifier | Bedfont SportsBedfont Sport | 1 - 1 | Herne BayHerne Bay |
| 19 Aug 23 | Qualifier | VCD AthleticVCD Athletic | 0 - 2 | Whitstable TownWhitstable To |
| 19 Aug 23 | Qualifier | Corinthian-CasualsCorinthian-Ca | 2 - 2 | UxbridgeUxbridge |
| 19 Aug 23 | Qualifier | Badshot LeaBadshot Lea | 2 - 3 | LancingLancing |
| 19 Aug 23 | Qualifier | Ashford UnitedAshford Unite | 2 - 3 | Beckenham TownBeckenham Tow |
| 19 Aug 23 | Qualifier | East Grinstead TownEast Grinstea | 1 - 3 | Hythe TownHythe Town |
| 19 Aug 23 | Qualifier | Wimborne TownWimborne Town | 0 - 0 | Paulton RoversPaulton Rover |
| 19 Aug 23 | Qualifier | Lymington TownLymington Tow | - | Bemerton Heath HarlequinsBemerton Heat |
| 19 Aug 23 | Qualifier | TavistockTavistock | 3 - 5 | Barnstaple TownBarnstaple To |
| 19 Aug 23 | Qualifier | Leighton TownLeighton Town | 1 - 0 | Enfield FCEnfield FC |
| 19 Aug 23 | Qualifier | Colney HeathColney Heath | 2 - 5 | FC RomaniaFC Romania |
| 19 Aug 23 | Qualifier | WidnesWidnes | 2 - 1 | TraffordTrafford |
| 19 Aug 23 | Qualifier | Kidsgrove AthleticKidsgrove Ath | 2 - 0 | Sutton Coldfield TownSutton Coldfi |
| 19 Aug 23 | Qualifier | WestfieldsWestfields | 1 - 2 | WorcesterWorcester |
| 19 Aug 23 | Qualifier | Rugby TownRugby Town | 5 - 1 | Newcastle TownNewcastle Tow |
| 19 Aug 23 | Qualifier | Spalding UnitedSpalding Unit | 1 - 1 | Shepshed DynamoShepshed Dyna |
| 19 Aug 23 | Qualifier | Stocksbridge Park SteelsStocksbridge | 0 - 1 | Cleethorpes TownCleethorpes T |
| 19 Aug 23 | Qualifier | Hullbridge SportsHullbridge Sp | 3 - 1 | Waltham AbbeyWaltham Abbey |
| 19 Aug 23 | Qualifier | GraysGrays | 1 - 1 | New SalamisNew Salamis |
| 19 Aug 23 | Qualifier | North LeighNorth Leigh | 1 - 2 | Bishops CleeveBishops Cleev |
| 19 Aug 23 | Qualifier | Ascot UnitedAscot United | 5 - 1 | Mangotsfield UnitedMangotsfield |
| 19 Aug 23 | Qualifier | RedhillRedhill | 1 - 0 | Ashford Town MiddlesexAshford Town |
| 22 Aug 23 | Qualifier | LeatherheadLeatherhead | 2 - 1 | South ParkSouth Park |
| 22 Aug 23 | Qualifier | Shepshed DynamoShepshed Dyna | 2 - 3 | Spalding UnitedSpalding Unit |
| 22 Aug 23 | Qualifier | Paulton RoversPaulton Rover | 0 - 2 | Wimborne TownWimborne Town |
| 22 Aug 23 | Qualifier | Dereham TownDereham Town | 2 - 1 | Ipswich WanderersIpswich Wande |
| 22 Aug 23 | Qualifier | Herne BayHerne Bay | 3 - 0 | Bedfont SportsBedfont Sport |
| 23 Aug 23 | Qualifier | New SalamisNew Salamis | 3 - 1 | GraysGrays |
| 23 Aug 23 | Qualifier | Barton RoversBarton Rovers | 3 - 2 | East Thurrock UnitedEast Thurrock |
| 23 Aug 23 | Qualifier | UxbridgeUxbridge | 5 - 1 | Corinthian-CasualsCorinthian-Ca |
| 02 Sep 23 | Qualifier | AshtonAshton | 3 - 1 | Dunston UTSDunston UTS |
| 02 Sep 23 | Qualifier | Prescot CablesPrescot Cable | 4 - 2 | North Ferriby FCNorth Ferriby |
| 02 Sep 23 | Qualifier | ClitheroeClitheroe | 1 - 2 | Hyde UnitedHyde United |
| 02 Sep 23 | Qualifier | ConsettConsett | 0 - 2 | Bradford Park AvenueBradford Park |
| 02 Sep 23 | Qualifier | Ossett UnitedOssett United | 0 - 3 | Marske UnitedMarske United |
| 02 Sep 23 | Qualifier | Warrington RylandsWarrington Ry | 1 - 0 | Stockton TownStockton Town |
| 02 Sep 23 | Qualifier | Bamber BridgeBamber Bridge | 0 - 4 | MossleyMossley |
| 02 Sep 23 | Qualifier | GuiseleyGuiseley | 0 - 0 | MarineMarine |
| 02 Sep 23 | Qualifier | Morpeth TownMorpeth Town | 3 - 2 | RadcliffeRadcliffe |
| 02 Sep 23 | Qualifier | Lancaster CityLancaster Cit | 2 - 1 | WittonWitton |
| 02 Sep 23 | Qualifier | RedditchRedditch | 5 - 0 | Boldmere St MichaelsBoldmere St M |
| 02 Sep 23 | Qualifier | Cleethorpes TownCleethorpes T | 2 - 1 | Hinckley Leicester RoadHinckley Leic |
| 02 Sep 23 | Qualifier | Lincoln UnitedLincoln Unite | 2 - 0 | Kidsgrove AthleticKidsgrove Ath |
| 02 Sep 23 | Qualifier | Rugby TownRugby Town | 0 - 2 | Barwell FCBarwell FC |
Abbey Hulton United
Albion Sports
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City of Liverpool
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Cornard United
Coton Green
Coventry Sphinx
Dereham Town
Downham Town
Droitwich Spa
East Grinstead Town
Eccleshill United
Ely City
Fareham Town
Frenford
Frickley Athletic
Golcar United
Great Wakering Rovers
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Hackney Wick
Halstead Town
Handsworth
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Haverhill Rovers
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Heybridge Swifts
Horbury Town
Hullbridge Sports
Ilford
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March Town United
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Parkgate
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