| 09 Aug 25 | r01        | Hertford TownHertford Town | 2 - 0 | Thame UnitedThame United |
| 09 Aug 25 | r01        | Aylesbury UnitedAylesbury Uni | 0 - 0 | Leverstock GreenLeverstock Gr |
| 09 Aug 25 | r01        | AFC DunstableAFC Dunstable | 2 - 2 | NorthwoodNorthwood |
| 09 Aug 25 | r01        | Milton Keynes IrishMilton Keynes | 2 - 4 | Welwyn Garden CityWelwyn Garden |
| 09 Aug 25 | r01        | Leighton TownLeighton Town | 2 - 1 | WareWare |
| 09 Aug 25 | r01        | StotfoldStotfold | 0 - 3 | Beaconsfield TownBeaconsfield |
| 09 Aug 25 | r01        | Rayners LaneRayners Lane | 0 - 0 | BiggleswadeBiggleswade |
| 09 Aug 25 | r01        | Barton RoversBarton Rovers | 2 - 0 | MarlowMarlow |
| 09 Aug 25 | r01        | HadleyHadley | 0 - 2 | Hitchin TownHitchin Town |
| 09 Aug 25 | r01        | Biggleswade TownBiggleswade T | 2 - 1 | EnfieldEnfield |
| 09 Aug 25 | r01        | Flackwell HeathFlackwell Hea | 4 - 0 | London LionsLondon Lions |