| 13 Dec 25 | r22        | Newmarket TownNewmarket Tow | 4 - 1 | Lowestoft TownLowestoft Tow |
| 13 Dec 25 | r22        | Downham TownDownham Town | 0 - 0 | Concord RangersConcord Range |
| 13 Dec 25 | r22        | Cambridge CityCambridge Cit | 0 - 0 | Brightlingsea RegentBrightlingsea |
| 13 Dec 25 | r22        | Waltham AbbeyWaltham Abbey | 3 - 2 | Bowers and PitseaBowers and Pi |
| 13 Dec 25 | r22        | Felixstowe and Walton UniFelixstowe an | 2 - 0 | WroxhamWroxham |
| 13 Dec 25 | r22        | WalthamstowWalthamstow | 2 - 0 | TakeleyTakeley |
| 13 Dec 25 | r22        | Maldon and TiptreeMaldon and Ti | 3 - 1 | GraysGrays |
| 13 Dec 25 | r22        | Witham TownWitham Town | 2 - 2 | RedbridgeRedbridge |
| 13 Dec 25 | r22        | TilburyTilbury | 2 - 0 | Heybridge SwiftsHeybridge Swi |
| 13 Dec 25 | r22        | GorlestonGorleston | 1 - 4 | Stanway RoversStanway Rover |
| 13 Dec 25 | r22        | Brantham AthleticBrantham Athl | 2 - 3 | Mildenhall TownMildenhall To |