| 21 Mar 23 |
r01        | West Chester United SCWest Chester | 1 - 3 | Ocean City NoreastersOcean City No |
| 22 Mar 23 |
r01        | El FarolitoEl Farolito | 3 - 0 | Inter San FranciscoInter San Fra |
| 22 Mar 23 |
r01        | AppalachianAppalachian | 2 - 2 | North Carolina Fusion U23North Carolin |
| 23 Mar 23 |
r01        | Tulsa AthleticsTulsa Athleti | 1 - 0 | Brazos Valley Cavalry FCBrazos Valley |
| 23 Mar 23 |
r01        | Chicago House ACChicago House | 1 - 0 | Bavarian UnitedBavarian Unit |
| 23 Mar 23 |
r01        | Beaman UnitedBeaman United | 0 - 3 | Des Moines MenaceDes Moines Me |
| 23 Mar 23 |
r01        | UDA Soccer NMSUUDA Soccer NM | 1 - 0 | Park City Red Wolves SCPark City Red |
| 23 Mar 23 |
r01        | Project 51OProject 51O | 0 - 3 | Crossfire RedmondCrossfire Red |
| 23 Mar 23 |
r01        | Ventura County FusionVentura Count | 1 - 4 | Capo FCCapo FC |
| 23 Mar 23 |
r01        | Cleveland SCCleveland SC | 1 - 0 | Lionsbridge FCLionsbridge F |
| 23 Mar 23 |
r01        | Jacksonville Armada U-23Jacksonville | 1 - 1 | Miami UnitedMiami United |
| 23 Mar 23 |
r01        | Hartford CityHartford City | 1 - 2 | Lansdowne BhoysLansdowne Bho |