| 28 Jun 25 |
r20        | Charlottesville BluesCharlottesvil | 7 - 0 | Virginia MaraudersVirginia Mara |
| 28 Jun 25 |
r20        | Lionsbridge FCLionsbridge F | 5 - 0 | Christos FCChristos FC |
| 28 Jun 25 |
r20        | SC United BantamsSC United Ban | 3 - 2 | Wake FCWake FC |
| 28 Jun 25 |
r20        | Annapolis BluesAnnapolis Blu | 2 - 0 | Patuxent Football AthletiPatuxent Foot |
| 29 Jun 25 |
r20        | Western Mass PioneersWestern Mass | 3 - 0 | Boston CityBoston City |
| 29 Jun 25 |
r20        | McKinney ChupacabrasMcKinney Chup | 2 - 0 | Red RiverRed River |
| 29 Jun 25 |
r20        | Dothan UnitedDothan United | 2 - 0 | Columbus UnitedColumbus Unit |
| 29 Jun 25 |
r20        | Long Island Rough RidersLong Island R | 4 - 2 | Hudson Valley HammersHudson Valley |
| 30 Jun 25 |
r20        | Albany RushAlbany Rush | 0 - 4 | AC ConnecticutAC Connecticu |
| 30 Jun 25 |
r20        | Black Rock FCBlack Rock FC | 1 - 1 | Seacoast United PhantomsSeacoast Unit |
| 01 Jul 25 |
r20        | Boston BoltsBoston Bolts | 0 - 0 | New England FCNew England F |
| 01 Jul 25 |
r20        | East Atlanta Dutch LionsEast Atlanta | 0 - 3 | Dothan UnitedDothan United |
| 02 Jul 25 |
r20        | Birmingham Legion 2Birmingham Le | 2 - 1 | Montgomery UnitedMontgomery Un |
| 02 Jul 25 |
r20        | Kings Hammer Sun CityKings Hammer | 1 - 0 | Weston FCWeston FC |
| 02 Jul 25 |
r20        | FC Miami CityFC Miami City | 5 - 0 | Miami ACMiami AC |
| 02 Jul 25 |
r20        | St. Petersburg FCSt. Petersbur | 1 - 1 | Sarasota ParadiseSarasota Para |
| 02 Jul 25 |
r20        | Northern Virginia FCNorthern Virg | 1 - 2 | Christos FCChristos FC |
| 07 Jul 25 |
r20        | Southern Soccer AcademySouthern Socc | 2 - 2 | Montgomery UnitedMontgomery Un |
| 10 Jul 25 |
r20        | Albion SC ColoradoAlbion SC Col | 6 - 1 | CISACISA |
| 10 Jul 25 |
r20        | Marin FC LegendsMarin FC Lege | 2 - 4 | San Francisco GlensSan Francisco |
| 10 Jul 25 |
r20        | Ventura County FusionVentura Count | 3 - 2 | Redlands FCRedlands FC |
| 10 Jul 25 |
r20        | San Francisco City FCSan Francisco | 1 - 2 | Project 51OProject 51O |
| 11 Jul 25 |
r20        | Flatirons RushFlatirons Rus | 1 - 2 | Colorado StormColorado Stor |
| 12 Jul 25 |
r20        | FC OlympiaFC Olympia | 1 - 2 | West Seattle JunctionWest Seattle |
| 12 Jul 25 |
r20        | Midlakes UnitedMidlakes Unit | 1 - 1 | Bigfoot FCBigfoot FC |
| 12 Jul 25 |
r20        | Asheville City SCAsheville Cit | 0 - 0 | Tennessee SCTennessee SC |
| 12 Jul 25 |
r20        | Fort Wayne FCFort Wayne FC | 4 - 0 | Kings Hammer FCKings Hammer |
| 13 Jul 25 |
r20        | Des Moines MenaceDes Moines Me | 4 - 3 | St. Louis AmbushSt. Louis Amb |
| 13 Jul 25 |
r20        | Springfield FCSpringfield F | 0 - 4 | Santafé WanderersSantafé Wande |
| 13 Jul 25 |
r20        | Peoria CityPeoria City | 3 - 1 | Sunflower StateSunflower Sta |
| 13 Jul 25 |
r20        | Cleveland ForceCleveland For | 1 - 3 | Steel CitySteel City |
| 13 Jul 25 |
r20        | Chicago City Dutch LionsChicago City | 0 - 2 | Rochester FCRochester FC |
| 13 Jul 25 |
r20        | St. Croix SCSt. Croix SC | 1 - 3 | RKC Third CoastRKC Third Coa |