| 15 Jun 25 | r14        | Denton DiablosDenton Diablo | 3 - 1 | Red RiverRed River |
| 15 Jun 25 | r14        | Dothan UnitedDothan United | 4 - 1 | Birmingham Legion 2Birmingham Le |
| 15 Jun 25 | r14        | Laredo HeatLaredo Heat | 3 - 1 | Houston FCHouston FC |
| 15 Jun 25 | r14        | Annapolis BluesAnnapolis Blu | 1 - 2 | Northern Virginia FCNorthern Virg |
| 15 Jun 25 | r14        | FC Miami CityFC Miami City | 2 - 3 | St. Petersburg FCSt. Petersbur |
| 15 Jun 25 | r14        | Cedar Stars RushCedar Stars R | 2 - 0 | New Jersey CopaNew Jersey Co |
| 17 Jun 25 | r14        | Twin City ToucansTwin City Tou | 2 - 1 | Corpus Christi FCCorpus Christ |
| 17 Jun 25 | r14        | Vermont GreenVermont Green | 2 - 0 | Western Mass PioneersWestern Mass |
| 17 Jun 25 | r14        | Manhattan SCManhattan SC | 3 - 4 | FC Motown IIFC Motown II |
| 17 Jun 25 | r14        | East Atlanta Dutch LionsEast Atlanta | - | Athens UnitedAthens United |
| 18 Jun 25 | r14        | Red RiverRed River | 0 - 2 | Louisiana KreweLouisiana Kre |
| 18 Jun 25 | r14        | West Chester United IIWest Chester | 3 - 1 | Lehigh Valley UnitedLehigh Valley |
| 18 Jun 25 | r14        | Birmingham Legion 2Birmingham Le | 1 - 1 | Southern Soccer AcademySouthern Socc |
| 18 Jun 25 | r14        | Long Island Rough RidersLong Island R | 4 - 0 | New Jersey CopaNew Jersey Co |
| 18 Jun 25 | r14        | Boston CityBoston City | 3 - 3 | Black Rock FCBlack Rock FC |
| 18 Jun 25 | r14        | Seacoast United PhantomsSeacoast Unit | 1 - 2 | Boston BoltsBoston Bolts |
| 18 Jun 25 | r14        | Charlotte EaglesCharlotte Eag | 3 - 4 | Salem CitySalem City |
| 18 Jun 25 | r14        | Nona FCNona FC | 1 - 0 | Brooke HouseBrooke House |
| 18 Jun 25 | r14        | AC ConnecticutAC Connecticu | 0 - 1 | New England FCNew England F |
| 21 Jun 25 | r14        | FC Capo IIFC Capo II | 10 - 0 | Coachella SCCoachella SC |
| 22 Jun 25 | r14        | Snohomish UnitedSnohomish Uni | 0 - 1 | Portland BangersPortland Bang |
| 22 Jun 25 | r14        | FC OlympiaFC Olympia | 1 - 1 | Midlakes UnitedMidlakes Unit |
| 22 Jun 25 | r14        | Redlands FCRedlands FC | 2 - 0 | FC TucsonFC Tucson |
| 22 Jun 25 | r14        | Utah UnitedUtah United | 2 - 0 | CISACISA |
| 22 Jun 25 | r14        | Project 51OProject 51O | 2 - 2 | San Juan SCSan Juan SC |
| 22 Jun 25 | r14        | West Seattle JunctionWest Seattle | 0 - 0 | Tacoma StarsTacoma Stars |
| 22 Jun 25 | r14        | Kings Hammer ColumbusKings Hammer | 2 - 1 | Lexington SC IILexington SC |
| 22 Jun 25 | r14        | RKC Third CoastRKC Third Coa | 3 - 2 | Rochester FCRochester FC |
| 22 Jun 25 | r14        | San Francisco City FCSan Francisco | 2 - 0 | Davis LegacyDavis Legacy |
| 23 Jun 25 | r14        | Minneapolis CityMinneapolis C | 5 - 1 | River LightRiver Light |
| 24 Jun 25 | r14        | Southern California EagleSouthern Cali | 2 - 2 | FC TucsonFC Tucson |
| 24 Jun 25 | r14        | Portland BangersPortland Bang | 1 - 1 | Lane UnitedLane United |
| 24 Jun 25 | r14        | Lansing City FootballLansing City | 2 - 3 | AFC Ann ArborAFC Ann Arbor |
| 25 Jun 25 | r14        | St. Louis AmbushSt. Louis Amb | 1 - 3 | Peoria CityPeoria City |
| 25 Jun 25 | r14        | Dayton Dutch LionsDayton Dutch | 2 - 3 | West Virginia UnitedWest Virginia |
| 25 Jun 25 | r14        | Cleveland ForceCleveland For | 0 - 2 | Akron CityAkron City |
| 25 Jun 25 | r14        | FC BuffaloFC Buffalo | 1 - 4 | Steel CitySteel City |
| 25 Jun 25 | r14        | Kalamazoo FCKalamazoo FC | 2 - 1 | Northern IndianaNorthern Indi |
| 25 Jun 25 | r14        | Kings Hammer ColumbusKings Hammer | 2 - 1 | Fort Wayne FCFort Wayne FC |
| 05 Jul 25 | r14        | Brevard SCBrevard SC | 6 - 3 | Brave SCBrave SC |