| 11 Jun 25 | r12        | Delaware FCDelaware FC | 1 - 1 | Reading UnitedReading Unite |
| 11 Jun 25 | r12        | Tobacco RoadTobacco Road | 2 - 3 | Charlotte EaglesCharlotte Eag |
| 11 Jun 25 | r12        | Virginia Beach UnitedVirginia Beac | 3 - 0 | Christos FCChristos FC |
| 11 Jun 25 | r12        | Sporting JAXSporting JAX | 6 - 2 | Brevard SCBrevard SC |
| 11 Jun 25 | r12        | Ironbound SCIronbound SC | 0 - 2 | Westchester FlamesWestchester F |
| 11 Jun 25 | r12        | Columbus UnitedColumbus Unit | 4 - 0 | East Atlanta Dutch LionsEast Atlanta |
| 12 Jun 25 | r12        | Fort Lauderdale UnitedFort Lauderda | 3 - 0 | Miami ACMiami AC |
| 12 Jun 25 | r12        | AC Houston SurAC Houston Su | 0 - 5 | GFI AcademyGFI Academy |
| 12 Jun 25 | r12        | Little Rock RangersLittle Rock R | 2 - 0 | Denton DiablosDenton Diablo |
| 12 Jun 25 | r12        | Birmingham Legion 2Birmingham Le | 0 - 7 | Asheville City SCAsheville Cit |
| 12 Jun 25 | r12        | Montgomery UnitedMontgomery Un | 1 - 2 | Tennessee SCTennessee SC |
| 12 Jun 25 | r12        | Lonestar SCLonestar SC | 2 - 0 | Corpus Christi FCCorpus Christ |
| 13 Jun 25 | r12        | Twin City ToucansTwin City Tou | 2 - 0 | AHFC RoyalsAHFC Royals |
| 13 Jun 25 | r12        | FC Motown IIFC Motown II | 5 - 2 | Staten Island AthleticStaten Island |
| 13 Jun 25 | r12        | Western Mass PioneersWestern Mass | 1 - 1 | Black Rock FCBlack Rock FC |
| 14 Jun 25 | r12        | Boston BoltsBoston Bolts | 2 - 2 | Albany RushAlbany Rush |
| 14 Jun 25 | r12        | New England FCNew England F | 1 - 3 | AC ConnecticutAC Connecticu |
| 15 Jun 25 | r12        | AMSG FCAMSG FC | 2 - 2 | Redlands FCRedlands FC |
| 15 Jun 25 | r12        | Coachella SCCoachella SC | 0 - 16 | FC TucsonFC Tucson |
| 15 Jun 25 | r12        | Tacoma StarsTacoma Stars | 2 - 3 | FC OlympiaFC Olympia |
| 15 Jun 25 | r12        | Utah UnitedUtah United | 1 - 0 | Colorado StormColorado Stor |
| 15 Jun 25 | r12        | Bigfoot FCBigfoot FC | 3 - 3 | Midlakes UnitedMidlakes Unit |
| 17 Jun 25 | r12        | Sueño FCSueño FC | 2 - 1 | River LightRiver Light |
| 17 Jun 25 | r12        | Portland BangersPortland Bang | 2 - 1 | Tacoma StarsTacoma Stars |
| 17 Jun 25 | r12        | Akron CityAkron City | 3 - 0 | FC BuffaloFC Buffalo |
| 17 Jun 25 | r12        | AFC Ann ArborAFC Ann Arbor | 2 - 1 | Midwest UnitedMidwest Unite |
| 17 Jun 25 | r12        | Lansing City FootballLansing City | 1 - 3 | Kalamazoo FCKalamazoo FC |
| 18 Jun 25 | r12        | Snohomish UnitedSnohomish Uni | 4 - 4 | Bigfoot FCBigfoot FC |
| 18 Jun 25 | r12        | Lane UnitedLane United | 4 - 1 | FC OlympiaFC Olympia |
| 18 Jun 25 | r12        | San Francisco GlensSan Francisco | 0 - 2 | Davis LegacyDavis Legacy |
| 18 Jun 25 | r12        | West Virginia UnitedWest Virginia | 1 - 2 | Kings Hammer ColumbusKings Hammer |
| 18 Jun 25 | r12        | Fort Wayne FCFort Wayne FC | 2 - 0 | Lexington SC IILexington SC |
| 19 Jun 25 | r12        | Virginia MaraudersVirginia Mara | 2 - 3 | Christos FCChristos FC |
| 19 Jun 25 | r12        | Springfield FCSpringfield F | 1 - 3 | Des Moines MenaceDes Moines Me |
| 19 Jun 25 | r12        | Minneapolis CityMinneapolis C | 3 - 1 | St. Croix SCSt. Croix SC |
| 19 Jun 25 | r12        | Colorado StormColorado Stor | 2 - 2 | Flatirons RushFlatirons Rus |
| 19 Jun 25 | r12        | Toledo VillaToledo Villa | 2 - 3 | Steel CitySteel City |
| 21 Jun 25 | r12        | Brooke HouseBrooke House | 1 - 2 | Nona FCNona FC |
| 23 Jun 25 | r12        | Kings Hammer FCKings Hammer | 2 - 2 | Dayton Dutch LionsDayton Dutch |