| 07 Jun 25 | r11        | Southern Soccer AcademySouthern Socc | 0 - 5 | Dothan UnitedDothan United |
| 08 Jun 25 | r11        | Hattiesburg FCHattiesburg F | 1 - 0 | Mississippi BrillaMississippi B |
| 08 Jun 25 | r11        | Louisiana KreweLouisiana Kre | 1 - 0 | Little Rock RangersLittle Rock R |
| 08 Jun 25 | r11        | Red RiverRed River | 1 - 1 | Denton DiablosDenton Diablo |
| 08 Jun 25 | r11        | Twin City ToucansTwin City Tou | 3 - 2 | Hill Country LobosHill Country |
| 08 Jun 25 | r11        | Long Island Rough RidersLong Island R | 1 - 3 | FC Motown IIFC Motown II |
| 08 Jun 25 | r11        | Cedar Stars RushCedar Stars R | 3 - 1 | Westchester FlamesWestchester F |
| 09 Jun 25 | r11        | Houston FCHouston FC | 0 - 0 | GFI AcademyGFI Academy |
| 09 Jun 25 | r11        | Brooke HouseBrooke House | 1 - 0 | Inter GainesvilleInter Gainesv |
| 10 Jun 25 | r11        | AC ConnecticutAC Connecticu | 1 - 2 | Western Mass PioneersWestern Mass |
| 10 Jun 25 | r11        | Black Rock FCBlack Rock FC | 0 - 2 | Vermont GreenVermont Green |
| 10 Jun 25 | r11        | Wake FCWake FC | 0 - 3 | SC United BantamsSC United Ban |
| 11 Jun 25 | r11        | Mississippi BrillaMississippi B | 2 - 2 | Red RiverRed River |
| 11 Jun 25 | r11        | West Chester United IIWest Chester | 1 - 4 | Ocean City Nor'eastersOcean City No |
| 11 Jun 25 | r11        | New Jersey CopaNew Jersey Co | 0 - 1 | Long Island Rough RidersLong Island R |
| 11 Jun 25 | r11        | Albany RushAlbany Rush | 1 - 2 | Seacoast United PhantomsSeacoast Unit |
| 11 Jun 25 | r11        | Kings Hammer Sun CityKings Hammer | 1 - 4 | St. Petersburg FCSt. Petersbur |
| 11 Jun 25 | r11        | Hudson Valley HammersHudson Valley | 4 - 0 | Manhattan SCManhattan SC |
| 11 Jun 25 | r11        | New England FCNew England F | 4 - 2 | Boston CityBoston City |
| 11 Jun 25 | r11        | Northern Virginia FCNorthern Virg | 4 - 1 | Patuxent Football AthletiPatuxent Foot |
| 11 Jun 25 | r11        | FC Miami CityFC Miami City | 4 - 2 | Sarasota ParadiseSarasota Para |
| 13 Jun 25 | r11        | City SCCity SC | 2 - 1 | FC TucsonFC Tucson |
| 13 Jun 25 | r11        | Davis LegacyDavis Legacy | 1 - 2 | Monterey Bay FC 2Monterey Bay |
| 13 Jun 25 | r11        | Utah UnitedUtah United | 1 - 2 | Colorado StormColorado Stor |
| 13 Jun 25 | r11        | Stars FCStars FC | 9 - 0 | Coachella SCCoachella SC |
| 14 Jun 25 | r11        | Portland BangersPortland Bang | 4 - 2 | West Seattle JunctionWest Seattle |
| 14 Jun 25 | r11        | Akron CityAkron City | 1 - 1 | Cleveland ForceCleveland For |
| 14 Jun 25 | r11        | Kalamazoo FCKalamazoo FC | 0 - 0 | AFC Ann ArborAFC Ann Arbor |
| 14 Jun 25 | r11        | Kings Hammer ColumbusKings Hammer | 1 - 2 | Dayton Dutch LionsDayton Dutch |
| 14 Jun 25 | r11        | Kings Hammer FCKings Hammer | 0 - 1 | Fort Wayne FCFort Wayne FC |
| 15 Jun 25 | r11        | Des Moines MenaceDes Moines Me | 3 - 1 | Peoria CityPeoria City |
| 15 Jun 25 | r11        | Santafé WanderersSantafé Wande | 4 - 3 | Springfield FCSpringfield F |
| 15 Jun 25 | r11        | Sunflower StateSunflower Sta | 3 - 2 | St. Louis AmbushSt. Louis Amb |
| 15 Jun 25 | r11        | San Francisco GlensSan Francisco | 1 - 1 | San Francisco City FCSan Francisco |
| 15 Jun 25 | r11        | Ballard FCBallard FC | 0 - 0 | Lane UnitedLane United |
| 15 Jun 25 | r11        | Flatirons RushFlatirons Rus | 0 - 0 | CISACISA |
| 15 Jun 25 | r11        | Almaden FCAlmaden FC | 1 - 2 | Project 51OProject 51O |
| 15 Jun 25 | r11        | Academica SCAcademica SC | 1 - 1 | Marin FC LegendsMarin FC Lege |
| 15 Jun 25 | r11        | Erie Sports CenterErie Sports C | 1 - 2 | Toledo VillaToledo Villa |
| 15 Jun 25 | r11        | Rochester FCRochester FC | 1 - 2 | Minneapolis CityMinneapolis C |
| 15 Jun 25 | r11        | RKC Third CoastRKC Third Coa | 5 - 0 | St. Croix SCSt. Croix SC |