| 07 Jun 25 | r10        | Morris EliteMorris Elite | 2 - 1 | Staten Island AthleticStaten Island |
| 07 Jun 25 | r10        | Weston FCWeston FC | 1 - 6 | St. Petersburg FCSt. Petersbur |
| 07 Jun 25 | r10        | Kings Hammer Sun CityKings Hammer | 2 - 2 | FC Miami CityFC Miami City |
| 07 Jun 25 | r10        | Black Rock FCBlack Rock FC | 1 - 0 | AC ConnecticutAC Connecticu |
| 07 Jun 25 | r10        | Vermont GreenVermont Green | 1 - 1 | Boston BoltsBoston Bolts |
| 07 Jun 25 | r10        | Charlotte Independence IICharlotte Ind | 2 - 1 | North Carolina FC U23North Carolin |
| 07 Jun 25 | r10        | Salem CitySalem City | 1 - 1 | SC United BantamsSC United Ban |
| 07 Jun 25 | r10        | Real Central New JerseyReal Central | 1 - 1 | West Chester United IIWest Chester |
| 07 Jun 25 | r10        | Delaware FCDelaware FC | 1 - 1 | Lehigh Valley UnitedLehigh Valley |
| 07 Jun 25 | r10        | Brave SCBrave SC | 1 - 0 | Nona FCNona FC |
| 07 Jun 25 | r10        | Sporting JAXSporting JAX | 1 - 0 | Inter GainesvilleInter Gainesv |
| 07 Jun 25 | r10        | Ironbound SCIronbound SC | 2 - 2 | New Jersey CopaNew Jersey Co |
| 07 Jun 25 | r10        | Annapolis BluesAnnapolis Blu | 0 - 2 | Lionsbridge FCLionsbridge F |
| 07 Jun 25 | r10        | Asheville City SCAsheville Cit | 5 - 0 | Montgomery UnitedMontgomery Un |
| 07 Jun 25 | r10        | Columbus UnitedColumbus Unit | 1 - 2 | Birmingham Legion 2Birmingham Le |
| 07 Jun 25 | r10        | Sarasota ParadiseSarasota Para | 2 - 0 | Miami ACMiami AC |
| 07 Jun 25 | r10        | Athens UnitedAthens United | - | Apotheos FCApotheos FC |
| 08 Jun 25 | r10        | AC Houston SurAC Houston Su | 0 - 2 | Laredo HeatLaredo Heat |
| 08 Jun 25 | r10        | Corpus Christi FCCorpus Christ | 2 - 0 | AHFC RoyalsAHFC Royals |
| 08 Jun 25 | r10        | New England FCNew England F | 2 - 0 | Albany RushAlbany Rush |
| 09 Jun 25 | r10        | Southern California EagleSouthern Cali | 2 - 6 | FC Capo IIFC Capo II |
| 10 Jun 25 | r10        | Marin FC LegendsMarin FC Lege | 3 - 2 | Davis LegacyDavis Legacy |
| 10 Jun 25 | r10        | Stars FCStars FC | 1 - 3 | AMSG FCAMSG FC |
| 11 Jun 25 | r10        | Kalamazoo FCKalamazoo FC | 0 - 5 | Midwest UnitedMidwest Unite |
| 11 Jun 25 | r10        | Kings Hammer ColumbusKings Hammer | 0 - 2 | Kings Hammer FCKings Hammer |
| 11 Jun 25 | r10        | FC Capo IIFC Capo II | 1 - 0 | Ventura County FusionVentura Count |
| 12 Jun 25 | r10        | Santafé WanderersSantafé Wande | 2 - 4 | Sunflower StateSunflower Sta |
| 12 Jun 25 | r10        | Albion SC ColoradoAlbion SC Col | 0 - 1 | Flatirons RushFlatirons Rus |
| 12 Jun 25 | r10        | St. Louis AmbushSt. Louis Amb | 3 - 1 | Springfield FCSpringfield F |
| 12 Jun 25 | r10        | Snohomish UnitedSnohomish Uni | 1 - 1 | Ballard FCBallard FC |
| 12 Jun 25 | r10        | Project 51OProject 51O | 1 - 1 | San Francisco GlensSan Francisco |
| 12 Jun 25 | r10        | Southern California EagleSouthern Cali | 2 - 3 | Redlands FCRedlands FC |
| 12 Jun 25 | r10        | Midlakes UnitedMidlakes Unit | 1 - 1 | West Seattle JunctionWest Seattle |
| 12 Jun 25 | r10        | Almaden FCAlmaden FC | 1 - 2 | San Francisco City FCSan Francisco |
| 13 Jun 25 | r10        | St. Croix SCSt. Croix SC | 2 - 3 | Rochester FCRochester FC |
| 13 Jun 25 | r10        | RKC Third CoastRKC Third Coa | 2 - 1 | River LightRiver Light |
| 13 Jun 25 | r10        | Oakland CountyOakland Count | 3 - 0 | Lansing City FootballLansing City |
| 14 Jun 25 | r10        | Erie Sports CenterErie Sports C | 0 - 1 | FC BuffaloFC Buffalo |
| 14 Jun 25 | r10        | Flint City BucksFlint City Bu | 2 - 0 | Northern IndianaNorthern Indi |
| 14 Jun 25 | r10        | Union FC MacombUnion FC Maco | 1 - 3 | Midwest UnitedMidwest Unite |