| 01 Jun 25 |
r08        | Tennessee SCTennessee SC | 3 - 3 | Montgomery UnitedMontgomery Un |
| 01 Jun 25 |
r08        | Laredo HeatLaredo Heat | 4 - 1 | Corpus Christi FCCorpus Christ |
| 01 Jun 25 |
r08        | East Atlanta Dutch LionsEast Atlanta | 0 - 5 | Asheville City SCAsheville Cit |
| 01 Jun 25 |
r08        | Inter GainesvilleInter Gainesv | 1 - 0 | Sporting JAXSporting JAX |
| 01 Jun 25 |
r08        | Weston FCWeston FC | 2 - 2 | FC Miami CityFC Miami City |
| 01 Jun 25 |
r08        | Manhattan SCManhattan SC | 0 - 1 | Cedar Stars RushCedar Stars R |
| 02 Jun 25 |
r08        | Ventura County FusionVentura Count | 3 - 0 | AMSG FCAMSG FC |
| 03 Jun 25 |
r08        | Boston BoltsBoston Bolts | 0 - 3 | Western Mass PioneersWestern Mass |
| 03 Jun 25 |
r08        | Seacoast United PhantomsSeacoast Unit | 0 - 1 | Black Rock FCBlack Rock FC |
| 04 Jun 25 |
r08        | Red RiverRed River | 2 - 4 | McKinney ChupacabrasMcKinney Chup |
| 04 Jun 25 |
r08        | West Chester United IIWest Chester | 4 - 1 | Reading UnitedReading Unite |
| 04 Jun 25 |
r08        | Virginia Beach UnitedVirginia Beac | 1 - 3 | Annapolis BluesAnnapolis Blu |
| 04 Jun 25 |
r08        | Virginia MaraudersVirginia Mara | 2 - 11 | Lionsbridge FCLionsbridge F |
| 04 Jun 25 |
r08        | Patuxent Football AthletiPatuxent Foot | 0 - 4 | Christos FCChristos FC |
| 04 Jun 25 |
r08        | Inter GainesvilleInter Gainesv | 2 - 3 | Brooke HouseBrooke House |
| 04 Jun 25 |
r08        | Weston FCWeston FC | 0 - 3 | Kings Hammer Sun CityKings Hammer |
| 04 Jun 25 |
r08        | Hudson Valley HammersHudson Valley | 1 - 0 | Cedar Stars RushCedar Stars R |
| 04 Jun 25 |
r08        | Albany RushAlbany Rush | 1 - 4 | Vermont GreenVermont Green |
| 05 Jun 25 |
r08        | Northern Virginia FCNorthern Virg | 3 - 1 | Charlottesville BluesCharlottesvil |
| 05 Jun 25 |
r08        | Almaden FCAlmaden FC | 1 - 3 | San Francisco GlensSan Francisco |
| 06 Jun 25 |
r08        | Flatirons RushFlatirons Rus | 2 - 1 | Utah UnitedUtah United |
| 06 Jun 25 |
r08        | Lane UnitedLane United | 2 - 0 | West Seattle JunctionWest Seattle |
| 06 Jun 25 |
r08        | Sueño FCSueño FC | 3 - 2 | Minneapolis CityMinneapolis C |
| 06 Jun 25 |
r08        | Midwest UnitedMidwest Unite | 2 - 1 | Oakland CountyOakland Count |
| 07 Jun 25 |
r08        | Ballard FCBallard FC | 1 - 0 | Portland BangersPortland Bang |
| 07 Jun 25 |
r08        | Midlakes UnitedMidlakes Unit | 3 - 2 | FC OlympiaFC Olympia |
| 07 Jun 25 |
r08        | Steel CitySteel City | 4 - 0 | Erie Sports CenterErie Sports C |
| 07 Jun 25 |
r08        | Sunflower StateSunflower Sta | 2 - 1 | Peoria CityPeoria City |
| 07 Jun 25 |
r08        | Lexington SC IILexington SC | 2 - 2 | Kings Hammer ColumbusKings Hammer |
| 07 Jun 25 |
r08        | St. Louis AmbushSt. Louis Amb | 0 - 2 | Santafé WanderersSantafé Wande |
| 07 Jun 25 |
r08        | West Virginia UnitedWest Virginia | 2 - 2 | Kings Hammer FCKings Hammer |
| 07 Jun 25 |
r08        | AFC Ann ArborAFC Ann Arbor | 3 - 2 | Flint City BucksFlint City Bu |
| 07 Jun 25 |
r08        | Fort Wayne FCFort Wayne FC | 4 - 2 | Dayton Dutch LionsDayton Dutch |