| 04 May 25 |
r01        | Coachella SCCoachella SC | 0 - 4 | Southern California EagleSouthern Cali |
| 05 May 25 |
r01        | Monterey Bay FC 2Monterey Bay | 0 - 1 | Project 51OProject 51O |
| 07 May 25 |
r01        | Midwest UnitedMidwest Unite | 0 - 2 | Union FC MacombUnion FC Maco |
| 10 May 25 |
r01        | Charlottesville BluesCharlottesvil | 0 - 0 | Northern Virginia FCNorthern Virg |
| 10 May 25 |
r01        | Virginia Beach UnitedVirginia Beac | 2 - 2 | Lionsbridge FCLionsbridge F |
| 10 May 25 |
r01        | AFC Ann ArborAFC Ann Arbor | 2 - 0 | Lansing City FootballLansing City |
| 11 May 25 |
r01        | Montgomery UnitedMontgomery Un | 1 - 0 | Dothan UnitedDothan United |
| 11 May 25 |
r01        | St. Louis AmbushSt. Louis Amb | 1 - 2 | Peoria CityPeoria City |
| 11 May 25 |
r01        | Apotheos FCApotheos FC | - | Athens UnitedAthens United |
| 11 May 25 |
r01        | Almaden FCAlmaden FC | 4 - 0 | Monterey Bay FC 2Monterey Bay |
| 11 May 25 |
r01        | AMSG FCAMSG FC | 2 - 2 | Southern California EagleSouthern Cali |
| 11 May 25 |
r01        | Union FC MacombUnion FC Maco | 2 - 1 | Kalamazoo FCKalamazoo FC |
| 12 May 25 |
r01        | Tacoma StarsTacoma Stars | 0 - 1 | Snohomish UnitedSnohomish Uni |
| 14 May 25 |
r01        | Birmingham Legion 2Birmingham Le | P - P | Dothan UnitedDothan United |
| 14 May 25 |
r01        | St. Petersburg FCSt. Petersbur | 4 - 1 | Kings Hammer Sun CityKings Hammer |
| 14 May 25 |
r01        | Midwest UnitedMidwest Unite | 1 - 2 | AFC Ann ArborAFC Ann Arbor |
| 15 May 25 |
r01        | San Francisco City FCSan Francisco | 3 - 2 | San Juan SCSan Juan SC |
| 15 May 25 |
r01        | Academica SCAcademica SC | 2 - 0 | Monterey Bay FC 2Monterey Bay |
| 15 May 25 |
r01        | Erie Sports CenterErie Sports C | 1 - 3 | Cleveland ForceCleveland For |
| 16 May 25 |
r01        | Boston CityBoston City | 0 - 9 | Western Mass PioneersWestern Mass |
| 16 May 25 |
r01        | Lehigh Valley UnitedLehigh Valley | 2 - 5 | West Chester United IIWest Chester |
| 17 May 25 |
r01        | Sunflower StateSunflower Sta | 2 - 3 | Springfield FCSpringfield F |
| 17 May 25 |
r01        | Kings Hammer ColumbusKings Hammer | 1 - 2 | Lexington SC IILexington SC |
| 17 May 25 |
r01        | River LightRiver Light | 5 - 3 | Chicago City Dutch LionsChicago City |
| 17 May 25 |
r01        | Christos FCChristos FC | 0 - 1 | Charlottesville BluesCharlottesvil |
| 17 May 25 |
r01        | Salem CitySalem City | 2 - 1 | Charlotte EaglesCharlotte Eag |
| 17 May 25 |
r01        | Southern Soccer AcademySouthern Socc | 0 - 2 | Birmingham Legion 2Birmingham Le |
| 18 May 25 |
r01        | Westchester FlamesWestchester F | 1 - 1 | Morris EliteMorris Elite |
| 18 May 25 |
r01        | Columbus UnitedColumbus Unit | 1 - 1 | Tennessee SCTennessee SC |
| 12 Jun 25 |
r01        | Virginia MaraudersVirginia Mara | 0 - 3 | Annapolis BluesAnnapolis Blu |