| 19 Mar 21 | r04        | Melbourne KnightsMelbourne Kni | 3 - 0 | Hume City FCHume City FC |
| 19 Mar 21 | r04        | Port Melbourne SCPort Melbourn | 0 - 0 | South Melbourne FCSouth Melbour |
| 19 Mar 21 | r04        | Green Gully SCGreen Gully S | 1 - 2 | Heidelberg United FCHeidelberg Un |
| 19 Mar 21 | r04        | Bentleigh Greens SCBentleigh Gre | 0 - 0 | St Albans Saints SCSt Albans Sai |
| 19 Mar 21 | r04        | Dandenong City SCDandenong Cit | 1 - 4 | Avondale FCAvondale FC |
| 20 Mar 21 | r04        | Altona Magic SCAltona Magic | 0 - 1 | Oakleigh Cannons FCOakleigh Cann |
| 21 Mar 21 | r04        | Eastern Lions SCEastern Lions | 1 - 2 | Dandenong Thunder SCDandenong Thu |