| 26 Aug 25 | r02        | Shrewsbury TownShrewsbury To | 3 - 1 | Chelsea U21Chelsea U21 |
| 16 Sep 25 | r02        | AFC WimbledonAFC Wimbledon | 3 - 1 | Crystal Palace U21Crystal Palac |
| 23 Sep 25 | r02        | Bolton WanderersBolton Wander | 3 - 0 | Manchester City U21Manchester Ci |
| 23 Sep 25 | r02        | Plymouth ArgylePlymouth Argy | 6 - 2 | Tottenham U21Tottenham U21 |
| 30 Sep 25 | r02        | Lincoln CityLincoln City | 3 - 0 | Manchester United U21Manchester Un |
| 30 Sep 25 | r02        | Notts CountyNotts County | 2 - 1 | BarnsleyBarnsley |
| 30 Sep 25 | r02        | Peterborough UnitedPeterborough | 4 - 2 | Aston Villa U21Aston Villa U |
| 30 Sep 25 | r02        | Harrogate TownHarrogate Tow | 1 - 0 | Huddersfield TownHuddersfield |
| 07 Oct 25 | r02        | ChesterfieldChesterfield | 1 - 0 | Burton AlbionBurton Albion |
| 07 Oct 25 | r02        | Wigan AthleticWigan Athleti | 1 - 2 | Wolverhampton Wanderers UWolverhampton |
| 07 Oct 25 | r02        | Salford CitySalford City | 3 - 1 | Stockport CountyStockport Cou |
| 07 Oct 25 | r02        | Grimsby TownGrimsby Town | 0 - 3 | Doncaster RoversDoncaster Rov |
| 07 Oct 25 | r02        | Rotherham UnitedRotherham Uni | 3 - 2 | Oldham AthleticOldham Athlet |
| 07 Oct 25 | r02        | Fleetwood TownFleetwood Tow | 4 - 0 | Leeds United U21Leeds United |
| 07 Oct 25 | r02        | Wycombe WanderersWycombe Wande | 3 - 1 | Fulham U21Fulham U21 |
| 07 Oct 25 | r02        | Leyton OrientLeyton Orient | 2 - 1 | Crawley TownCrawley Town |
| 07 Oct 25 | r02        | StevenageStevenage | 2 - 1 | BromleyBromley |
| 07 Oct 25 | r02        | ReadingReading | 1 - 0 | Milton Keynes DonsMilton Keynes |
| 07 Oct 25 | r02        | WalsallWalsall | 0 - 1 | Northampton TownNorthampton T |
| 07 Oct 25 | r02        | Cheltenham TownCheltenham To | 0 - 1 | Bristol RoversBristol Rover |
| 07 Oct 25 | r02        | BarrowBarrow | 1 - 2 | Tranmere RoversTranmere Rove |
| 07 Oct 25 | r02        | Colchester UnitedColchester Un | 2 - 1 | GillinghamGillingham |
| 07 Oct 25 | r02        | Cardiff CityCardiff City | 0 - 1 | Newport CountyNewport Count |
| 07 Oct 25 | r02        | Accrington StanleyAccrington St | 0 - 2 | Port ValePort Vale |
| 14 Oct 25 | r02        | BlackpoolBlackpool | 2 - 1 | Nottingham Forest U21Nottingham Fo |
| 14 Oct 25 | r02        | Crewe AlexandraCrewe Alexand | 2 - 0 | Liverpool U21Liverpool U21 |
| 14 Oct 25 | r02        | Bradford CityBradford City | 5 - 1 | Everton U21Everton U21 |
| 15 Oct 25 | r02        | Mansfield TownMansfield Tow | 2 - 2 | Newcastle United U21Newcastle Uni |
| 21 Oct 25 | r02        | BarnetBarnet | 3 - 2 | Cambridge UnitedCambridge Uni |
| 28 Oct 25 | r02        | Swindon TownSwindon Town | 2 - 3 | West Ham United U21West Ham Unit |
| 28 Oct 25 | r02        | Luton TownLuton Town | 3 - 1 | Brighton and Hove Albion Brighton and |
| 28 Oct 25 | r02        | Exeter CityExeter City | 4 - 3 | Arsenal U21Arsenal U21 |