| 26 Aug 25 | r01        | Oldham AthleticOldham Athlet | 1 - 5 | Manchester City U21Manchester Ci |
| 26 Aug 25 | r01        | Bristol RoversBristol Rover | 4 - 4 | Tottenham U21Tottenham U21 |
| 02 Sep 25 | r01        | Wigan AthleticWigan Athleti | 0 - 2 | Salford CitySalford City |
| 02 Sep 25 | r01        | Wycombe WanderersWycombe Wande | 1 - 2 | Colchester UnitedColchester Un |
| 02 Sep 25 | r01        | GillinghamGillingham | 4 - 1 | Fulham U21Fulham U21 |
| 02 Sep 25 | r01        | Peterborough UnitedPeterborough | 1 - 3 | Leyton OrientLeyton Orient |
| 02 Sep 25 | r01        | AFC WimbledonAFC Wimbledon | 1 - 5 | StevenageStevenage |
| 02 Sep 25 | r01        | Swindon TownSwindon Town | 3 - 2 | ReadingReading |
| 02 Sep 25 | r01        | Shrewsbury TownShrewsbury To | 1 - 3 | WalsallWalsall |
| 02 Sep 25 | r01        | Plymouth ArgylePlymouth Argy | 2 - 0 | Cheltenham TownCheltenham To |
| 02 Sep 25 | r01        | Stockport CountyStockport Cou | 5 - 3 | Wolverhampton Wanderers UWolverhampton |
| 02 Sep 25 | r01        | Doncaster RoversDoncaster Rov | 2 - 1 | Everton U21Everton U21 |
| 02 Sep 25 | r01        | Lincoln CityLincoln City | 3 - 0 | Notts CountyNotts County |
| 02 Sep 25 | r01        | Bolton WanderersBolton Wander | 1 - 0 | Rotherham UnitedRotherham Uni |
| 02 Sep 25 | r01        | Fleetwood TownFleetwood Tow | 2 - 2 | Accrington StanleyAccrington St |
| 02 Sep 25 | r01        | Port ValePort Vale | 4 - 1 | Leeds United U21Leeds United |
| 02 Sep 25 | r01        | Crewe AlexandraCrewe Alexand | 7 - 1 | ChesterfieldChesterfield |
| 02 Sep 25 | r01        | Burton AlbionBurton Albion | 2 - 0 | Liverpool U21Liverpool U21 |
| 02 Sep 25 | r01        | Luton TownLuton Town | 4 - 1 | BarnetBarnet |
| 02 Sep 25 | r01        | Bradford CityBradford City | 2 - 1 | Grimsby TownGrimsby Town |
| 02 Sep 25 | r01        | Huddersfield TownHuddersfield | 6 - 2 | Newcastle United U21Newcastle Uni |
| 02 Sep 25 | r01        | BromleyBromley | 3 - 3 | Crystal Palace U21Crystal Palac |
| 09 Sep 25 | r01        | Tranmere RoversTranmere Rove | 2 - 2 | Nottingham Forest U21Nottingham Fo |
| 09 Sep 25 | r01        | Mansfield TownMansfield Tow | 0 - 1 | Harrogate TownHarrogate Tow |
| 16 Sep 25 | r01        | BlackpoolBlackpool | 5 - 0 | BarrowBarrow |
| 16 Sep 25 | r01        | Exeter CityExeter City | 0 - 1 | Cardiff CityCardiff City |
| 16 Sep 25 | r01        | Milton Keynes DonsMilton Keynes | 1 - 5 | West Ham United U21West Ham Unit |
| 16 Sep 25 | r01        | Crawley TownCrawley Town | 4 - 2 | Aston Villa U21Aston Villa U |
| 23 Sep 25 | r01        | Newport CountyNewport Count | 1 - 2 | Arsenal U21Arsenal U21 |
| 23 Sep 25 | r01        | Northampton TownNorthampton T | 3 - 0 | Chelsea U21Chelsea U21 |
| 21 Oct 25 | r01        | BarnsleyBarnsley | 5 - 2 | Manchester United U21Manchester Un |
| 05 Nov 25 | r01        | Cambridge UnitedCambridge Uni | 1 - 0 | Brighton and Hove Albion Brighton and |