| 31 Aug 21 | r02        | Cambridge UnitedCambridge Uni | 4 - 1 | Oxford UtdOxford Utd |
| 31 Aug 21 | r02        | CharltonCharlton | 6 - 1 | CrawleyCrawley |
| 31 Aug 21 | r02        | Bristol RoversBristol Rover | 2 - 0 | CheltenhamCheltenham |
| 31 Aug 21 | r02        | Forest Green RoversForest Green | 1 - 1 | NorthamptonNorthampton |
| 31 Aug 21 | r02        | WycombeWycombe | 1 - 3 | Aston Villa U21Aston Villa U |
| 31 Aug 21 | r02        | Sheffield WedsSheffield Wed | 3 - 0 | Newcastle United U21Newcastle Uni |
| 31 Aug 21 | r02        | BurtonBurton | 1 - 2 | Milton Keynes DonsMilton Keynes |
| 31 Aug 21 | r02        | StevenageStevenage | 3 - 4 | Tottenham U21Tottenham U21 |
| 31 Aug 21 | r02        | Sutton UnitedSutton United | 3 - 0 | Crystal Palace U21Crystal Palac |
| 07 Sep 21 | r02        | SwindonSwindon | 2 - 1 | Arsenal U21Arsenal U21 |
| 30 Nov 21 | r02        | CharltonCharlton | 2 - 1 | Aston Villa U21Aston Villa U |