| 17 Mar 21 | r16        | Akwa UnitedAkwa United | 1 - 0 | Ifeanyi UbahIfeanyi Ubah |
| 17 Mar 21 | r16        | Rivers UnitedRivers United | 2 - 0 | MFMMFM |
| 17 Mar 21 | r16        | Adamawa UnitedAdamawa Unite | 1 - 1 | Abia WarriorsAbia Warriors |
| 17 Mar 21 | r16        | Kano PillarsKano Pillars | 2 - 0 | Kwara UnitedKwara United |
| 17 Mar 21 | r16        | HeartlandHeartland | 2 - 0 | Wikki TouristsWikki Tourist |
| 17 Mar 21 | r16        | Warri WolvesWarri Wolves | 0 - 0 | Jigawa Golden StarsJigawa Golden |
| 18 Mar 21 | r16        | Plateau UnitedPlateau Unite | 3 - 1 | Sunshine StarsSunshine Star |
| 18 Mar 21 | r16        | Nasarawa UnitedNasarawa Unit | 3 - 0 | DakkadaDakkada |
| 18 Mar 21 | r16        | Katsina UnitedKatsina Unite | 2 - 0 | Rangers InternationalRangers Inter |
| 02 Jun 21 | r16        | EnyimbaEnyimba | 2 - 1 | Lobi StarsLobi Stars |