| 14 Jun 25 | r16        | Loudoun UnitedLoudoun Unite | 3 - 2 | Detroit City FCDetroit City |
| 14 Jun 25 | r16        | Hartford AthleticHartford Athl | 1 - 2 | Charleston BatteryCharleston Ba |
| 14 Jun 25 | r16        | Indy ElevenIndy Eleven | 1 - 0 | Pittsburgh RiverhoundsPittsburgh Ri |
| 14 Jun 25 | r16        | Lexington SCLexington SC | 2 - 1 | New Mexico UnitedNew Mexico Un |
| 14 Jun 25 | r16        | Rhode Island FCRhode Island | 2 - 1 | North Carolina FCNorth Carolin |
| 14 Jun 25 | r16        | Tampa Bay RowdiesTampa Bay Row | 2 - 1 | Miami FCMiami FC |
| 15 Jun 25 | r16        | Louisville CityLouisville Ci | 4 - 2 | Birmingham LegionBirmingham Le |
| 15 Jun 25 | r16        | FC TulsaFC Tulsa | 1 - 1 | Phoenix RisingPhoenix Risin |
| 15 Jun 25 | r16        | Las Vegas LightsLas Vegas Lig | 2 - 0 | Monterey Bay FCMonterey Bay |
| 15 Jun 25 | r16        | Sacramento RepublicSacramento Re | 0 - 0 | San AntonioSan Antonio |
| 01 Nov 25 | r16        | Pittsburgh RiverhoundsPittsburgh Ri | 0 - 0 | Hartford AthleticHartford Athl |
| 01 Nov 25 | r16        | Louisville CityLouisville Ci | 0 - 1 | Detroit City FCDetroit City |
| 01 Nov 25 | r16        | North Carolina FCNorth Carolin | 1 - 0 | Loudoun UnitedLoudoun Unite |
| 02 Nov 25 | r16        | FC TulsaFC Tulsa | 1 - 0 | Colorado Springs SwitchbaColorado Spri |
| 02 Nov 25 | r16        | New Mexico UnitedNew Mexico Un | 2 - 0 | San AntonioSan Antonio |
| 02 Nov 25 | r16        | El Paso LocomotiveEl Paso Locom | 0 - 1 | Phoenix RisingPhoenix Risin |
| 02 Nov 25 | r16        | Charleston BatteryCharleston Ba | 0 - 0 | Rhode Island FCRhode Island |
| 03 Nov 25 | r16        | Sacramento RepublicSacramento Re | 0 - 0 | Orange County SCOrange County |