| 05 Oct 24 | r37        | Charleston BatteryCharleston Ba | 2 - 2 | Detroit City FCDetroit City |
| 06 Oct 24 | r37        | Memphis 901Memphis 901 | 1 - 1 | Oakland RootsOakland Roots |
| 06 Oct 24 | r37        | San AntonioSan Antonio | 2 - 1 | Sacramento RepublicSacramento Re |
| 06 Oct 24 | r37        | Tulsa RoughnecksTulsa Roughne | 0 - 1 | El Paso LocomotiveEl Paso Locom |
| 06 Oct 24 | r37        | Las Vegas LightsLas Vegas Lig | 1 - 1 | Orange County SCOrange County |
| 06 Oct 24 | r37        | Birmingham LegionBirmingham Le | 2 - 3 | North Carolina FCNorth Carolin |
| 08 Oct 24 | r37        | Loudoun UnitedLoudoun Unite | 0 - 1 | Indy ElevenIndy Eleven |
| 09 Oct 24 | r37        | North Carolina FCNorth Carolin | 2 - 0 | Tulsa RoughnecksTulsa Roughne |
| 10 Oct 24 | r37        | El Paso LocomotiveEl Paso Locom | 2 - 2 | San AntonioSan Antonio |
| 10 Oct 24 | r37        | Sacramento RepublicSacramento Re | 2 - 2 | Monterey Bay FCMonterey Bay |