| 08 Oct 22 | r12        | Bath CityBath City | 2 - 3 | Hungerford TownHungerford To |
| 08 Oct 22 | r12        | ChelmsfordChelmsford | 2 - 0 | CheshuntCheshunt |
| 08 Oct 22 | r12        | DoverDover | 3 - 1 | Braintree TownBraintree Tow |
| 08 Oct 22 | r12        | Dulwich HamletDulwich Hamle | 1 - 2 | DartfordDartford |
| 08 Oct 22 | r12        | Eastbourne BoroughEastbourne Bo | 5 - 1 | WeymouthWeymouth |
| 08 Oct 22 | r12        | Ebbsfleet UnitedEbbsfleet Uni | 5 - 0 | Taunton TownTaunton Town |
| 08 Oct 22 | r12        | Slough TownSlough Town | 0 - 2 | Hemel Hempstead Town FCHemel Hempste |
| 08 Oct 22 | r12        | St Albans CitySt Albans Cit | 1 - 1 | Chippenham Town FCChippenham To |
| 08 Oct 22 | r12        | Tonbridge AngelsTonbridge Ang | 2 - 2 | Oxford CityOxford City |
| 08 Oct 22 | r12        | WorthingWorthing | 1 - 2 | FarnboroughFarnborough |
| 08 Oct 22 | r12        | Hampton and RichmondHampton and | 1 - 1 | Welling UnitedWelling Unite |
| 08 Oct 22 | r12        | Havant and WaterloovilleHavant and W | 2 - 0 | Concord RangersConcord Range |