| 04 Oct 25 | r10        | AFC Telford UnitedAFC Telford U | 1 - 3 | AFC FyldeAFC Fylde |
| 04 Oct 25 | r10        | BuxtonBuxton | 1 - 0 | DarlingtonDarlington |
| 04 Oct 25 | r10        | ChesterChester | 2 - 2 | Merthyr TownMerthyr Town |
| 04 Oct 25 | r10        | King's Lynn TownKing's Lynn T | 2 - 3 | Scarborough AthleticScarborough A |
| 04 Oct 25 | r10        | LeamingtonLeamington | 1 - 2 | HerefordHereford |
| 04 Oct 25 | r10        | MacclesfieldMacclesfield | 0 - 1 | MarineMarine |
| 04 Oct 25 | r10        | Oxford CityOxford City | 1 - 2 | Curzon AshtonCurzon Ashton |
| 04 Oct 25 | r10        | Peterborough SportsPeterborough | 2 - 1 | Kidderminster HarriersKidderminster |
| 04 Oct 25 | r10        | RadcliffeRadcliffe | 3 - 1 | Alfreton TownAlfreton Town |
| 04 Oct 25 | r10        | South ShieldsSouth Shields | 2 - 0 | ChorleyChorley |
| 04 Oct 25 | r10        | Spennymoor TownSpennymoor To | 2 - 0 | SouthportSouthport |
| 04 Oct 25 | r10        | Worksop TownWorksop Town | 0 - 2 | Bedford TownBedford Town |