| 06 Jan 24 | r27        | Bishops StortfordBishops Stort | 1 - 3 | Blyth SpartansBlyth Spartan |
| 06 Jan 24 | r27        | Alfreton TownAlfreton Town | 2 - 0 | ChorleyChorley |
| 06 Jan 24 | r27        | Chester CityChester City | 0 - 1 | Rushall OlympicRushall Olymp |
| 06 Jan 24 | r27        | CurzonCurzon | 0 - 0 | Farsley CelticFarsley Celti |
| 06 Jan 24 | r27        | Gloucester CityGloucester Ci | 2 - 2 | SouthportSouthport |
| 06 Jan 24 | r27        | Kings Lynn TownKings Lynn To | 0 - 1 | Darlington F.C.Darlington F. |
| 06 Jan 24 | r27        | Scarborough AthleticScarborough A | 3 - 0 | HerefordHereford |
| 06 Jan 24 | r27        | ScunthorpeScunthorpe | 2 - 2 | Boston United FCBoston United |
| 06 Jan 24 | r27        | South ShieldsSouth Shields | 1 - 1 | Warrington TownWarrington To |
| 06 Jan 24 | r27        | Spennymoor Town FCSpennymoor To | 0 - 1 | Brackley TownBrackley Town |
| 06 Jan 24 | r27        | TamworthTamworth | 2 - 0 | Buxton F.C.Buxton F.C. |
| 27 Feb 24 | r27        | BanburyBanbury | 0 - 3 | Peterborough SportsPeterborough |