| 02 Sep 23 | r07        | Alfreton TownAlfreton Town | 2 - 2 | ScunthorpeScunthorpe |
| 02 Sep 23 | r07        | Boston United FCBoston United | 3 - 1 | Blyth SpartansBlyth Spartan |
| 02 Sep 23 | r07        | Brackley TownBrackley Town | 2 - 0 | Warrington TownWarrington To |
| 02 Sep 23 | r07        | Buxton F.C.Buxton F.C. | 5 - 0 | Darlington F.C.Darlington F. |
| 02 Sep 23 | r07        | ChorleyChorley | 2 - 1 | Bishops StortfordBishops Stort |
| 02 Sep 23 | r07        | CurzonCurzon | 0 - 1 | TamworthTamworth |
| 02 Sep 23 | r07        | Farsley CelticFarsley Celti | 0 - 0 | Kings Lynn TownKings Lynn To |
| 02 Sep 23 | r07        | HerefordHereford | 0 - 1 | SouthportSouthport |
| 02 Sep 23 | r07        | Rushall OlympicRushall Olymp | 1 - 0 | Peterborough SportsPeterborough |
| 02 Sep 23 | r07        | Scarborough AthleticScarborough A | 1 - 1 | Gloucester CityGloucester Ci |
| 02 Sep 23 | r07        | South ShieldsSouth Shields | 4 - 0 | BanburyBanbury |
| 02 Sep 23 | r07        | Spennymoor Town FCSpennymoor To | 1 - 1 | Chester CityChester City |