| 07 Jan 23 | r27        | AFC FyldeAFC Fylde | 5 - 0 | BanburyBanbury |
| 07 Jan 23 | r27        | AFC Telford UnitedAFC Telford U | 1 - 1 | Spennymoor Town FCSpennymoor To |
| 07 Jan 23 | r27        | Alfreton TownAlfreton Town | 1 - 0 | Farsley CelticFarsley Celti |
| 07 Jan 23 | r27        | Blyth SpartansBlyth Spartan | 0 - 0 | Kings Lynn TownKings Lynn To |
| 07 Jan 23 | r27        | Boston United FCBoston United | 2 - 2 | Gloucester CityGloucester Ci |
| 07 Jan 23 | r27        | Brackley TownBrackley Town | 1 - 1 | Chester CityChester City |
| 07 Jan 23 | r27        | Bradford Park AvenueBradford Park | 1 - 1 | Kettering TownKettering Tow |
| 07 Jan 23 | r27        | CurzonCurzon | 2 - 1 | Buxton F.C.Buxton F.C. |
| 07 Jan 23 | r27        | Darlington F.C.Darlington F. | 0 - 3 | ChorleyChorley |
| 07 Jan 23 | r27        | HerefordHereford | 2 - 0 | Scarborough AthleticScarborough A |
| 07 Jan 23 | r27        | LeamingtonLeamington | 0 - 0 | Kidderminster HarriersKidderminster |
| 07 Jan 23 | r27        | Peterborough SportsPeterborough | 2 - 1 | SouthportSouthport |