| 11 Oct 25 | r13        | FC United of ManchesterFC United of | 1 - 0 | Hebburn TownHebburn Town |
| 11 Oct 25 | r13        | GuiseleyGuiseley | 1 - 4 | Ilkeston TownIlkeston Town |
| 11 Oct 25 | r13        | Bamber BridgeBamber Bridge | 4 - 0 | Rushall OlympicRushall Olymp |
| 11 Oct 25 | r13        | Ashton UnitedAshton United | 1 - 4 | Whitby TownWhitby Town |
| 11 Oct 25 | r13        | Warrington RylandsWarrington Ry | 4 - 3 | Morpeth TownMorpeth Town |
| 11 Oct 25 | r13        | Stocksbridge Park SteelsStocksbridge | 2 - 1 | Warrington TownWarrington To |
| 11 Oct 25 | r13        | Leek TownLeek Town | 0 - 1 | Lancaster CityLancaster Cit |
| 11 Oct 25 | r13        | Cleethorpes TownCleethorpes T | 1 - 1 | Prescot CablesPrescot Cable |
| 21 Oct 25 | r13        | WorkingtonWorkington | 1 - 2 | Gainsborough TrinityGainsborough |
| 11 Nov 25 | r13        | Hednesford TownHednesford To | 2 - 1 | Hyde UnitedHyde United |