| 20 Sep 25 | r11        | GuiseleyGuiseley | 3 - 1 | Morpeth TownMorpeth Town |
| 20 Sep 25 | r11        | Ashton UnitedAshton United | 1 - 3 | Lancaster CityLancaster Cit |
| 20 Sep 25 | r11        | Warrington RylandsWarrington Ry | 4 - 2 | Hyde UnitedHyde United |
| 20 Sep 25 | r11        | Hednesford TownHednesford To | 2 - 0 | Gainsborough TrinityGainsborough |
| 20 Sep 25 | r11        | Cleethorpes TownCleethorpes T | 1 - 2 | Stockton TownStockton Town |
| 20 Sep 25 | r11        | Leek TownLeek Town | 1 - 2 | Ilkeston TownIlkeston Town |
| 20 Sep 25 | r11        | WorkingtonWorkington | 1 - 1 | Prescot CablesPrescot Cable |
| 20 Sep 25 | r11        | Stocksbridge Park SteelsStocksbridge | 1 - 2 | Hebburn TownHebburn Town |
| 21 Oct 25 | r11        | FC United of ManchesterFC United of | 3 - 1 | Rushall OlympicRushall Olymp |
| 13 Jan 26 | r11        | Bamber BridgeBamber Bridge | 1 - 2 | Warrington TownWarrington To |