| 09 Aug 25 | r01        | FC United of ManchesterFC United of | 2 - 0 | Leek TownLeek Town |
| 09 Aug 25 | r01        | Gainsborough TrinityGainsborough | 0 - 3 | Ashton UnitedAshton United |
| 09 Aug 25 | r01        | Bamber BridgeBamber Bridge | 2 - 0 | GuiseleyGuiseley |
| 09 Aug 25 | r01        | Rushall OlympicRushall Olymp | 1 - 1 | Warrington RylandsWarrington Ry |
| 09 Aug 25 | r01        | Warrington TownWarrington To | 0 - 0 | Cleethorpes TownCleethorpes T |
| 09 Aug 25 | r01        | Hyde UnitedHyde United | 1 - 0 | Lancaster CityLancaster Cit |
| 09 Aug 25 | r01        | Morpeth TownMorpeth Town | 1 - 2 | Ilkeston TownIlkeston Town |
| 09 Aug 25 | r01        | Prescot CablesPrescot Cable | 0 - 0 | Stockton TownStockton Town |
| 09 Aug 25 | r01        | WorkingtonWorkington | 1 - 2 | Whitby TownWhitby Town |
| 09 Aug 25 | r01        | Hebburn TownHebburn Town | 0 - 1 | Hednesford TownHednesford To |