| 02 Oct 21 | r12        | Lancaster CityLancaster Cit | 0 - 2 | GainsboroughGainsborough |
| 02 Oct 21 | r12        | MatlockMatlock | 1 - 0 | FC United of ManchesterFC United of |
| 02 Oct 21 | r12        | Nantwich TownNantwich Town | 0 - 0 | South ShieldsSouth Shields |
| 02 Oct 21 | r12        | Scarborough AthleticScarborough A | 1 - 1 | Mickleover SportsMickleover Sp |
| 02 Oct 21 | r12        | StalybridgeStalybridge | 2 - 1 | Stafford RangersStafford Rang |
| 02 Oct 21 | r12        | WittonWitton | 1 - 4 | Grantham TownGrantham Town |
| 16 Oct 21 | r12        | Basford Utd.Basford Utd. | 2 - 0 | Hyde UnitedHyde United |
| 16 Oct 21 | r12        | Basford Utd.Basford Utd. | 2 - 0 | Hyde UnitedHyde United |
| 02 Nov 21 | r12        | Bamber BridgeBamber Bridge | 3 - 0 | Whitby TownWhitby Town |
| 09 Nov 21 | r12        | AshtonAshton | 3 - 1 | RadcliffeRadcliffe |
| 09 Nov 21 | r12        | Buxton F.C.Buxton F.C. | 2 - 0 | Atherton CollieriesAtherton Coll |
| 08 Mar 22 | r12        | Morpeth TownMorpeth Town | 2 - 0 | Warrington TownWarrington To |