| 17 Aug 19 | r01  | Nantwich TownNantwich Town | 2 - 1 | GainsboroughGainsborough |
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| 20 Aug 19 | r02 | GainsboroughGainsborough | 2 - 0 | Whitby TownWhitby Town |
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| 24 Aug 19 | r03 | GainsboroughGainsborough | 0 - 0 | Warrington TownWarrington To |
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| 26 Aug 19 | r04 | MatlockMatlock | 2 - 1 | GainsboroughGainsborough |
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| 31 Aug 19 | r05 | GainsboroughGainsborough | 2 - 4 | Lancaster CityLancaster Cit |
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| 14 Sep 19 | r08 | WittonWitton | 1 - 0 | GainsboroughGainsborough |
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| 24 Sep 19 | r09 | South ShieldsSouth Shields | 2 - 0 | GainsboroughGainsborough |
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| 28 Sep 19 | r10 | GainsboroughGainsborough | 2 - 2 | Mickleover SportsMickleover Sp |
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| 01 Oct 19 | r11 | GainsboroughGainsborough | 4 - 1 | Bamber BridgeBamber Bridge |
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| 05 Oct 19 | r13 | Basford Utd.Basford Utd. | 4 - 3 | GainsboroughGainsborough |
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| 12 Oct 19 | r14 | GainsboroughGainsborough | 0 - 2 | Atherton CollieriesAtherton Coll |
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| 15 Oct 19 | r15 | GainsboroughGainsborough | 0 - 0 | Buxton F.C.Buxton F.C. |
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| 19 Oct 19 | r16 | AshtonAshton | 3 - 1 | GainsboroughGainsborough |
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| 02 Nov 19 | r17 | FC United of ManchesterFC United of | 2 - 2 | GainsboroughGainsborough |
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| 16 Nov 19 | r18 | GainsboroughGainsborough | 0 - 1 | Morpeth TownMorpeth Town |
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| 07 Dec 19 | r21 | GainsboroughGainsborough | 2 - 3 | Scarborough AthleticScarborough A |
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| 14 Dec 19 | r22 | Hyde UnitedHyde United | 1 - 1 | GainsboroughGainsborough |
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| 21 Dec 19 | r23 | GainsboroughGainsborough | 0 - 0 | Nantwich TownNantwich Town |
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| 26 Dec 19 | r24 | Grantham TownGrantham Town | 1 - 5 | GainsboroughGainsborough |
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| 01 Jan 20 | r25 | GainsboroughGainsborough | 2 - 1 | MatlockMatlock |
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| 04 Jan 20 | r26 | Whitby TownWhitby Town | 2 - 2 | GainsboroughGainsborough |
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| 11 Jan 20 | r27 | GainsboroughGainsborough | 3 - 1 | WittonWitton |
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| 18 Jan 20 | r28 | RadcliffeRadcliffe | 0 - 2 | GainsboroughGainsborough |
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| 25 Jan 20 | r29 | Atherton CollieriesAtherton Coll | 1 - 3 | GainsboroughGainsborough |
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| 01 Feb 20 | r30 | GainsboroughGainsborough | 1 - 0 | Basford Utd.Basford Utd. |
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| 04 Feb 20 | r06 | StalybridgeStalybridge | 1 - 2 | GainsboroughGainsborough |
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| 08 Feb 20 | r31 | Buxton F.C.Buxton F.C. | 3 - 3 | GainsboroughGainsborough |
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| 15 Feb 20 | r20 | GainsboroughGainsborough | 1 - 3 | StalybridgeStalybridge |
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| 10 Mar 20 | r36 | Stafford RangersStafford Rang | 2 - 2 | GainsboroughGainsborough |
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| 14 Mar 20 | r35 | Bamber BridgeBamber Bridge | - | GainsboroughGainsborough |
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| 21 Mar 20 | r12 | GainsboroughGainsborough | - | Stafford RangersStafford Rang |
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| 24 Mar 20 | r32 | GainsboroughGainsborough | - | AshtonAshton |
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| 28 Mar 20 | r37 | Scarborough AthleticScarborough A | - | GainsboroughGainsborough |
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| 04 Apr 20 | r38 | GainsboroughGainsborough | - | Hyde UnitedHyde United |
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| 07 Apr 20 | r33 | Mickleover SportsMickleover Sp | - | GainsboroughGainsborough |
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| 11 Apr 20 | r39 | Warrington TownWarrington To | - | GainsboroughGainsborough |
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| 13 Apr 20 | r40 | GainsboroughGainsborough | - | Grantham TownGrantham Town |
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| 18 Apr 20 | r41 | GainsboroughGainsborough | - | FC United of ManchesterFC United of |
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| 25 Apr 20 | r42 | Morpeth TownMorpeth Town | - | GainsboroughGainsborough |
Team 2020-2021 Schedules
Alfreton Town
Ashton United
Avro
Bamber Bridge
Bury
Cleethorpes Town
Curzon Ashton
Emley
FC United of Manchester
Gainsborough Trinity
Guiseley
Hyde United
Ilkeston Town
Lancaster City
Leek Town
Quorn
Redcar Athletic
Stockton Town
Warrington Rylands
Warrington Town
Whitby Town
Workington
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