| 12 Oct 19 | r14        | FC United of ManchesterFC United of | 7 - 0 | Buxton F.C.Buxton F.C. |
| 12 Oct 19 | r14        | GainsboroughGainsborough | 0 - 2 | Atherton CollieriesAtherton Coll |
| 12 Oct 19 | r14        | Grantham TownGrantham Town | 2 - 2 | Hyde UnitedHyde United |
| 12 Oct 19 | r14        | Lancaster CityLancaster Cit | 3 - 0 | AshtonAshton |
| 12 Oct 19 | r14        | MatlockMatlock | 3 - 1 | Mickleover SportsMickleover Sp |
| 12 Oct 19 | r14        | Morpeth TownMorpeth Town | 1 - 1 | Basford Utd.Basford Utd. |
| 12 Oct 19 | r14        | Nantwich TownNantwich Town | 0 - 1 | RadcliffeRadcliffe |
| 12 Oct 19 | r14        | Scarborough AthleticScarborough A | 1 - 1 | Stafford RangersStafford Rang |
| 12 Oct 19 | r14        | StalybridgeStalybridge | 3 - 2 | Bamber BridgeBamber Bridge |
| 12 Oct 19 | r14        | Whitby TownWhitby Town | 0 - 1 | Warrington TownWarrington To |
| 12 Oct 19 | r14        | WittonWitton | 3 - 1 | South ShieldsSouth Shields |