| 01 Nov 24 | r01        | TamworthTamworth | 1 - 0 | Huddersfield TownHuddersfield |
| 01 Nov 24 | r01        | Notts CountyNotts County | 5 - 1 | Alfreton TownAlfreton Town |
| 02 Nov 24 | r01        | RotherhamRotherham | 1 - 3 | CheltenhamCheltenham |
| 02 Nov 24 | r01        | BarrowBarrow | 0 - 1 | DoncasterDoncaster |
| 02 Nov 24 | r01        | ExeterExeter | 5 - 3 | BarnetBarnet |
| 02 Nov 24 | r01        | CarlisleCarlisle | 0 - 2 | WiganWigan |
| 02 Nov 24 | r01        | WycombeWycombe | 3 - 2 | YorkYork |
| 02 Nov 24 | r01        | BradfordBradford | 3 - 1 | AldershotAldershot |
| 02 Nov 24 | r01        | WorthingWorthing | 0 - 2 | MorecambeMorecambe |
| 02 Nov 24 | r01        | BurtonBurton | 1 - 0 | Scarborough AthleticScarborough A |
| 02 Nov 24 | r01        | TranmereTranmere | 1 - 2 | OldhamOldham |
| 02 Nov 24 | r01        | RochdaleRochdale | 3 - 4 | BromleyBromley |
| 02 Nov 24 | r01        | WalsallWalsall | 2 - 1 | BoltonBolton |
| 02 Nov 24 | r01        | GrimsbyGrimsby | 0 - 1 | WealdstoneWealdstone |
| 02 Nov 24 | r01        | Bristol RoversBristol Rover | 3 - 1 | Weston-super-Mare FCWeston-super- |
| 02 Nov 24 | r01        | StockportStockport | 2 - 1 | Forest Green RoversForest Green |
| 02 Nov 24 | r01        | ReadingReading | 2 - 0 | Fleetwood TownFleetwood Tow |
| 02 Nov 24 | r01        | StevenageStevenage | 1 - 1 | GuiseleyGuiseley |
| 02 Nov 24 | r01        | SwindonSwindon | 2 - 1 | ColchesterColchester |
| 02 Nov 24 | r01        | Salford CitySalford City | 2 - 1 | ShrewsburyShrewsbury |
| 02 Nov 24 | r01        | CreweCrewe | 0 - 1 | Dagenham and RedbridgeDagenham and |
| 02 Nov 24 | r01        | Port ValePort Vale | 1 - 3 | BarnsleyBarnsley |
| 02 Nov 24 | r01        | ChesterfieldChesterfield | 3 - 1 | HorshamHorsham |
| 02 Nov 24 | r01        | SouthendSouthend | 3 - 4 | CharltonCharlton |
| 02 Nov 24 | r01        | WokingWoking | 0 - 1 | Cambridge UnitedCambridge Uni |
| 02 Nov 24 | r01        | GillinghamGillingham | 0 - 2 | BlackpoolBlackpool |
| 02 Nov 24 | r01        | Tonbridge AngelsTonbridge Ang | 1 - 4 | Harborough TownHarborough To |
| 02 Nov 24 | r01        | NewportNewport | 2 - 4 | PeterboroPeterboro |
| 02 Nov 24 | r01        | Brackley TownBrackley Town | 0 - 0 | Braintree TownBraintree Tow |
| 02 Nov 24 | r01        | Hednesford TownHednesford To | 4 - 4 | GainsboroughGainsborough |
| 02 Nov 24 | r01        | Solihull MoorsSolihull Moor | 3 - 0 | MaidstoneMaidstone |
| 02 Nov 24 | r01        | Rushall OlympicRushall Olymp | 0 - 2 | AccringtonAccrington |
| 02 Nov 24 | r01        | Maidenhead UnitedMaidenhead Un | 1 - 2 | CrawleyCrawley |
| 02 Nov 24 | r01        | NorthamptonNorthampton | 1 - 2 | Kettering TownKettering Tow |
| 03 Nov 24 | r01        | Milton Keynes DonsMilton Keynes | 0 - 2 | AFC WimbledonAFC Wimbledon |
| 03 Nov 24 | r01        | Sutton UnitedSutton United | 0 - 1 | BirminghamBirmingham |
| 03 Nov 24 | r01        | CurzonCurzon | 0 - 4 | MansfieldMansfield |
| 03 Nov 24 | r01        | Boreham WoodBoreham Wood | 2 - 2 | Leyton OrientLeyton Orient |
| 03 Nov 24 | r01        | Harrogate Town FCHarrogate Tow | 1 - 0 | WrexhamWrexham |
| 04 Nov 24 | r01        | Chesham UnitedChesham Unite | 0 - 4 | LincolnLincoln |