| 04 Oct 25 | r11        | Milton Keynes DonsMilton Keynes | 3 - 2 | GillinghamGillingham |
| 04 Oct 25 | r11        | Newport CountyNewport Count | 0 - 1 | Swindon TownSwindon Town |
| 04 Oct 25 | r11        | BarnetBarnet | 2 - 0 | Accrington StanleyAccrington St |
| 04 Oct 25 | r11        | BarrowBarrow | 0 - 0 | Shrewsbury TownShrewsbury To |
| 04 Oct 25 | r11        | BromleyBromley | 3 - 3 | Tranmere RoversTranmere Rove |
| 04 Oct 25 | r11        | Cambridge UnitedCambridge Uni | 3 - 1 | Crawley TownCrawley Town |
| 04 Oct 25 | r11        | Cheltenham TownCheltenham To | 2 - 0 | Fleetwood TownFleetwood Tow |
| 04 Oct 25 | r11        | Colchester UnitedColchester Un | 6 - 2 | ChesterfieldChesterfield |
| 04 Oct 25 | r11        | Notts CountyNotts County | 3 - 1 | Oldham AthleticOldham Athlet |
| 04 Oct 25 | r11        | Salford CitySalford City | 0 - 2 | Grimsby TownGrimsby Town |
| 04 Oct 25 | r11        | WalsallWalsall | 2 - 1 | Bristol RoversBristol Rover |
| 06 Oct 25 | r11        | Harrogate TownHarrogate Tow | 1 - 2 | Crewe AlexandraCrewe Alexand |